慢性光化性皮炎患者外周血T细胞亚群和血清IL-17、IL-22、Fas、Fasl检测
发布时间：2018-07-16 23:28
【摘要】：目的：检测慢性光化性皮炎外周血T淋巴细胞亚群及血清IL-17、IL-22、sFas、sFasL的表达,探讨慢性光化性皮炎的发病机制。方法：1.研究对象的纳入：根据Noriis Hawk标准及1992年上海华山医院的临床诊断标准,收集24例符合以上标准的慢性光化性皮炎患者及随机抽取14名健康体检者。2.血液制品的采集及制备：a.T细胞亚群的标本收集：分别自肘静脉采集慢性光化性皮炎患者和健康体检者外周静脉血5ml,肝素抗凝并立即送检。b. IL-17、IL-22、Fas、FasL的标本收集：分别自肘静脉采集慢性光化性皮炎患者和健康体检者外周静脉血5ml,室温静置2小时后离心lOmin (3500r/min),收集上层血清1m1,于-20℃冰箱保存。3.血液制品的检测：a.T细胞亚群的检测：根据试剂盒说明书用免疫荧光单克隆抗体(CD4-FITC/CD8-PE/CD3-PE-Cy5)充分标记收集的抗凝血,采用Beckman-Coulter FC-500流式细胞仪分别检测慢性光化性皮炎患者及健康体检者外周血T淋巴细胞亚群。b. IL-17、IL-22、Fas、FasL的检测：根据试剂说明书准备试剂,配制标准品液,通过加待测血清、洗板、水浴、洗板、酶标、孵育、洗板、暗反应等,最后用酶标仪在450nm处测吸光值。分别检测慢性光化性皮炎患者及健康体检者外周血IL-17和IL-22、外周血凋亡蛋白sFas、sFasL水平。对24例符合纳入标准的慢性光化性皮炎患者及14名健康体检者分别用流式细胞术和ABC-ELISA双抗体夹心法检测慢性光化性皮炎患者外周血T淋巴细胞亚群、Th17相关因子、IL-17和IL-22、外周血凋亡蛋白sFas、sFasL水平,采用SPSS13.0软件对数据统计分析。结果：1.外周血T淋巴细胞亚群：慢性光化性皮炎患者组CD3+CD4+明显低于健康体检者组CD3+CD4+,经统计学分析(P0.05)有统计学差异。慢性光化性皮炎患者组CD3+CD8+明显高于健康体检者组CD3+CD8~+,经统计学分析(P0.05)有统计学差异。慢性光化性皮炎患者组CD3+CD4+/CD3+CD8+明显低于健康体检者组CD3+CD4+/CD3+CD8+,经统计学分析(P0.05)有统计学差异。慢性光化性皮炎患者组CD3+与健康体检者组CD3+相比,经统计学分析(P0.05)无统计学差异。2.血清IL-17、IL-22、Fas、FasL:慢性光化性皮炎患者组IL-22明显高于健康体检者组IL-22,经统计学分析(P0.05)有统计学差异。慢性光化性皮炎患者组sFas明显高于健康体检者组sFas,经统计学分析(P0.05)有统计学差异。慢性光化性皮炎患者组sFasl明显高于健康体检者组sFasl,经统计学分析(P0.05)有统计学差异。慢性光化性皮炎患者组IL-17与健康体检者组IL-17相比,经统计学分析(P0.05)无统计学差异。结论：1.慢性光化性皮炎患者T淋巴细胞表达失衡,免疫系统紊乱,CD8~+T淋巴细胞参与了慢性光化性皮炎的迟发型超敏反应。2.IL-22参与了慢性光化性皮炎的慢性炎症反应,促进了慢性光化性皮炎的发生发展。IL-17在陈旧性慢性光化性皮炎中并没有起到抗炎及致敏作用。3.细胞凋亡的异常在慢性光化性皮炎的发生发展中起到一定的作用。
[Abstract]:Objective : To investigate the expression of serum IL - 17 , IL - 22 , sFas and sFasL in peripheral blood of chronic actinic dermatitis and to explore the pathogenesis of chronic actinic dermatitis . The peripheral venous blood of IL - 17 , IL - 22 , Fas and FasL was collected from the peripheral venous blood of patients with chronic actinic dermatitis and healthy physical examination respectively from the elbow vein . After standing for 2 hours at room temperature , the supernatant was centrifuged for 30min ( 3500 r / min ) . The upper layer of serum 1m1 was collected and stored in the refrigerator at -20 鈩，
本文编号：2128032
[Abstract]:Objective : To investigate the expression of serum IL - 17 , IL - 22 , sFas and sFasL in peripheral blood of chronic actinic dermatitis and to explore the pathogenesis of chronic actinic dermatitis . The peripheral venous blood of IL - 17 , IL - 22 , Fas and FasL was collected from the peripheral venous blood of patients with chronic actinic dermatitis and healthy physical examination respectively from the elbow vein . After standing for 2 hours at room temperature , the supernatant was centrifuged for 30min ( 3500 r / min ) . The upper layer of serum 1m1 was collected and stored in the refrigerator at -20 鈩，
本文编号：2128032
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