白癜风患者血清及疱液中IL-22、肝及干细胞生长因子水平测定
发布时间：2018-08-06 10:49
【摘要】： 目的：检测白癜风患者血清及疱液中白细胞介素-22(Interleukin-22, IL-22)、肝细胞生长因子(Hepatocyte Growth Factor,HGF)及干细胞生长因子(Stem Cell Factor,SCF)水平,并分析处于不同发病阶段及经过治疗后不同疗效的白癜风患者上述因子水平变化情况,探讨上述因子在白癜风发病过程中的可能作用。 方法：收集2008年6月至2009年9月间于青岛大学医学院附属医院皮肤科就诊的白癜风患者为实验组,并以我院健康体检中心的健康人群为对照组。采用双抗体夹心酶联免疫吸附试验法(ELISA法)对56例患者及20例健康志愿者血清及其中15例接受表皮移植的患者皮损疱液中IL-22、HGF、SCF因子水平进行测定。同时采用综合疗法对全程接受治疗的18例患者血清中三种因子水平进行监测,观察三种因子的水平变化情况,数据采用SPSS3.0软件进行统计分析。 结果：进展期患者组、稳定期患者组、对照组血清中IL-22水平依次降低,且两两组间差异均有统计学意义(P均0.05),而患者组与进展期患者组及患者组与稳定期患者组间血清中IL-22水平差异未见有统计学意义(P0.05)。进展期患者组血清中HGF水平低于对照组,差异有统计学意义(P0.05),其余各组间HGF水平比较差异均未见有统计学意义(P均0.05)。患者组、进展期患者组、稳定期患者组血清中SCF水平差异均未见统计学意义(P均0.05)。接受表皮移植患者皮损疱液中IL-22含量显著高于供皮区水平,差异有统计学意义(P0.05)。接受表皮移植患者皮损疱液中HGF水平低于供皮区水平,差异有统计学意义(P0.05)。接受表皮移植患者皮损区疱液SCF水平显著高于供皮区,差异有统计学意义(P0.05)。18例患者经系统治疗后,疗效较好患者血清中IL-22水平与治疗前比较下降明显,差异有统计学意义(P0.05)。而疗效较差患者血清中IL-22水平差异未见统计学意义(P0.05),不同疗效患者血清中HGF、SCF水平差异均未见统计学意义(P均0.05)。 结论：IL-22可能在白癜风发病及加重过程中起重要作用,IL-22水平变化有可能成为预测白癜风患者病情进展及评价治疗疗效的指标之一。白癜风患者血清及皮损疱液中IL-22、HGF及SCF因子水平异常可能参与了白癜风的发病过程,白癜风患者体内HGF及SCF通路可能与白癜风的发病有关,同时两条通路可能与IL-22有一定联系。
[Abstract]:Objective: to detect the levels of interleukin-22 (IL-22), hepatocyte growth factor (Hepatocyte Growth) and stem cell growth factor (Stem Cell) in serum and bullous fluid of patients with vitiligo. The changes of these factors in patients with vitiligo at different stages and after treatment were analyzed, and the possible role of these factors in the pathogenesis of vitiligo was discussed. Methods: the patients with vitiligo were collected from June 2008 to September 2009 in the Department of Dermatology affiliated Hospital of Qingdao University Medical College as the experimental group and the healthy people in the health examination center of our hospital as the control group. Double antibody sandwich enzyme linked immunosorbent assay (ELISA) was used to determine the level of IL-22 HGF factor in the bullous fluid of 56 patients and 20 healthy volunteers and 15 of them received epidermal transplantation. At the same time, the serum levels of three factors in 18 patients were monitored by comprehensive therapy, and the changes of the three factors were observed. The data were analyzed by SPSS3.0 software. Results: the serum levels of IL-22 in advanced patients, stable patients and control groups decreased in turn. The difference between the two groups was statistically significant (P 0.05), but there was no significant difference in serum IL-22 levels between the patients group and the advanced group and between the patient group and the stable group (P0.05). The serum HGF level in the advanced group was lower than that in the control group (P0.05), but there was no significant difference in the HGF level among the other groups (P0.05). There was no significant difference in serum SCF levels among patients, advanced patients and stable patients (P < 0. 05). The content of IL-22 in epidermal graft patients with blister fluid was significantly higher than that in donor skin area, the difference was statistically significant (P0.05). The level of HGF in epidermis grafts was lower than that in donor area (P0.05). The SCF level of bullous fluid in epidermal transplant patients was significantly higher than that in donor skin area (P0.05). After systemic treatment, the serum IL-22 level of 18 patients with better curative effect was significantly lower than that of before treatment (P0.05). There was no significant difference in serum IL-22 levels in patients with poor efficacy (P0.05), and no significant difference in serum IL-22 levels in patients with different therapeutic effects (P0.05). Conclusion the level of IL-22 may play an important role in the pathogenesis and aggravation of vitiligo. The changes of IL-22 level may be one of the indexes to predict the progression of vitiligo and evaluate the therapeutic effect. The abnormal levels of IL-22 HGF and SCF in serum and bullous fluid may be involved in the pathogenesis of vitiligo. The HGF and SCF pathways in vitiligo patients may be related to the pathogenesis of vitiligo, and the two pathways may be related to IL-22.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R758.41
本文编号：2167500
[Abstract]:Objective: to detect the levels of interleukin-22 (IL-22), hepatocyte growth factor (Hepatocyte Growth) and stem cell growth factor (Stem Cell) in serum and bullous fluid of patients with vitiligo. The changes of these factors in patients with vitiligo at different stages and after treatment were analyzed, and the possible role of these factors in the pathogenesis of vitiligo was discussed. Methods: the patients with vitiligo were collected from June 2008 to September 2009 in the Department of Dermatology affiliated Hospital of Qingdao University Medical College as the experimental group and the healthy people in the health examination center of our hospital as the control group. Double antibody sandwich enzyme linked immunosorbent assay (ELISA) was used to determine the level of IL-22 HGF factor in the bullous fluid of 56 patients and 20 healthy volunteers and 15 of them received epidermal transplantation. At the same time, the serum levels of three factors in 18 patients were monitored by comprehensive therapy, and the changes of the three factors were observed. The data were analyzed by SPSS3.0 software. Results: the serum levels of IL-22 in advanced patients, stable patients and control groups decreased in turn. The difference between the two groups was statistically significant (P 0.05), but there was no significant difference in serum IL-22 levels between the patients group and the advanced group and between the patient group and the stable group (P0.05). The serum HGF level in the advanced group was lower than that in the control group (P0.05), but there was no significant difference in the HGF level among the other groups (P0.05). There was no significant difference in serum SCF levels among patients, advanced patients and stable patients (P < 0. 05). The content of IL-22 in epidermal graft patients with blister fluid was significantly higher than that in donor skin area, the difference was statistically significant (P0.05). The level of HGF in epidermis grafts was lower than that in donor area (P0.05). The SCF level of bullous fluid in epidermal transplant patients was significantly higher than that in donor skin area (P0.05). After systemic treatment, the serum IL-22 level of 18 patients with better curative effect was significantly lower than that of before treatment (P0.05). There was no significant difference in serum IL-22 levels in patients with poor efficacy (P0.05), and no significant difference in serum IL-22 levels in patients with different therapeutic effects (P0.05). Conclusion the level of IL-22 may play an important role in the pathogenesis and aggravation of vitiligo. The changes of IL-22 level may be one of the indexes to predict the progression of vitiligo and evaluate the therapeutic effect. The abnormal levels of IL-22 HGF and SCF in serum and bullous fluid may be involved in the pathogenesis of vitiligo. The HGF and SCF pathways in vitiligo patients may be related to the pathogenesis of vitiligo, and the two pathways may be related to IL-22.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R758.41
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