抑制性受体ILT3、ILT4在麻风病的免疫缺陷中的作用机制

发布时间：2018-08-26 17:51

【摘要】：目的麻风病是由麻风分枝杆菌引起的一种慢性传染病。目前研究示与麻风病人同等情况接触下,有且仅有少部分人发病,这是因为麻风病人体内的自身细胞免疫缺陷程度各不相同,可是其自身细胞免疫缺陷的确切机制尚不明确。所以麻风病是研究宿主天然免疫功能与病原体相互作用的理想模型。目前有不少研究表明ILTs既参与先天性免疫,也参与适应性免疫,在免疫应答中既有负性调节作用,亦有正性调节作用。也有研究表明高表达或强制性表达的ILT3、ILT4可诱导DC耐受,从而使机体产生免疫耐受。故我们推测,麻风患者外周血中CD14+单核细胞表面ILT3、ILT4是否高表达,其高表达与麻风病发生、发展及各型别之间的转化是否有联系?现今麻风病同ILTs之间的联系国内外罕见报告,为了研究上述问题,本实验研究通过检测各型麻风患者外周血CD14+单核细胞表面ILT3、ILT4双阳性表达百分比,以及正常人的外周血单核细胞分别与多茵型(MB)、少菌型(PB)两类麻风患者的血清以及正常对照者血清培养,从而揭示ILT3、ILT4和麻风病之间的关系,为预防及治疗麻风病提供理论依据、麻风疫苗的开发及研制提供新思路。方法标本来源于昆明医科大学院第二附属医院皮肤科及检验科。其中TT组的麻风患者4例,BT组的麻风患者11例,BB组的麻风患者3例,BL组麻风患者12例,LL组麻风患者10例,正常对照组40例。运用流式细胞术分别检测上述6个实验组外周血CD14+单核细胞上ILT3、ILT4双阳性的百分比。另外,再取正常人的外周血单核细胞分别与多菌型(MB)14例、少菌型(PB)6例两类共20例麻风患者的血清以及20例正常对照者血清培养,并采用流式细胞技术测定外周血CD14+单核细胞上ILT3、ILT4双阳性的百分比。而后用SPSS19.0软件包对资料进行统计分析。结果1.五组麻风患者的外周血CD14+单核细胞上的ILT3、ILT4的双阳性表达高于正常对照组,且表达百分比高低为(正常对照组ILT3、ILT4双阳性百分比)(TT组ILT3、ILT4双阳性百分比)(BT组ILT3、ILT4双阳性百分比)(BB组ILT3、ILT4双阳性百分比)(BL组ILT3、ILT4双阳性百分比)(LL组ILT3、ILT4双阳性百分比)。2.CD14+单核细胞表面的ILT3、ILT4在各组培养后的表达百分比率从高到低的顺序为多菌型麻风(MB)血清+正常人CD14+单核细胞少菌型(PB)血清+正常人CD14+单核细胞正常人血清+正常人CD14+单核细胞。结论1.麻风患者外周血中CD14+单核细胞表面ILT3、ILT4双阳性表达部分比率远高于正常人。2.ILT3和ILT4在五组麻风患者外周血CD14+单核细胞上的表达水平为TT组BT组BB组BL组LL组。3.为探明麻风患者血清中的可溶性因子对ILT3和ILT4在外周血CD14+单核细胞表达有上调作用,将其分别进行培养。ILT3、ILT4在培养后的表达百分比率从高到低的顺序为[多菌型麻风(MB)血清+正常人CD14+单核细胞][少菌型(PB)血清+正常人CD14+单核细胞][正常人血清+正常人CD14+单核细胞],故此可说明麻风患者血清中的可溶性因子的确存在上调CD14+单核细胞表面ILT3、ILT4表达的作用。4.CD14+单核细胞表面ILT3、ILT4的表达百分比率MB组PB组,并由此说明ILT3、ILT4表达水平较高时,麻风菌含量较高,自身免疫水平较低下。
[Abstract]:Objective Leprosy is a chronic infectious disease caused by Mycobacterium leprae. Current studies have shown that leprosy occurs in only a few people under the same conditions as leprosy patients. This is because the degree of cellular immune deficiency in leprosy patients varies, but the exact mechanism of cellular immune deficiency is still unclear. Wind sickness is an ideal model for studying the interaction between host innate immune function and pathogen.Many studies have shown that ILTs are involved in both innate and adaptive immunity.They have both negative and positive effects on immune response.Some studies have shown that high or compulsory expression of ILT3 and ILT4 can induce DC tolerance. Therefore, we speculate that the high expression of CD14 + monocyte surface ILT3 and ILT4 in the peripheral blood of leprosy patients is related to the occurrence, development and transformation of leprosy among different types. To study the relationship between ILT3, ILT4 and leprosy by detecting the expression of ILT3 and ILT4 on the surface of CD14 + monocytes in peripheral blood of leprosy patients, and the serum culture of normal persons, leprosy patients and normal controls. Methods The specimens were collected from the Department of Dermatology and Laboratory of the Second Affiliated Hospital of Kunming Medical University. There were 4 cases of leprosy in TT group, 11 cases of leprosy in BT group, 3 cases of leprosy in BB group, 12 cases of leprosy in BL group, 10 cases of leprosy in LL group. 40 cases in control group were examined by flow cytometry for the percentage of double positive of ILT3 and ILT4 on CD14 + monocytes in peripheral blood of the above six experimental groups. The percentage of double positive ILT3 and ILT4 on CD14+ monocytes in peripheral blood was measured by flow cytometry, and the data were analyzed by SPSS19.0 software package. Results 1. The double positive expression of ILT3 and ILT4 on CD14+ monocytes in peripheral blood of the five groups of leprosy patients was higher than that in the normal control group, and the percentage of expression was high or low (ILT3, ILT in the normal control group). Four double positive percentages (double positive percentages of ILT3 and ILT4 in TT group) (double positive percentages of ILT3 and ILT4 in BT group) (double positive percentages of ILT3 and ILT4 in BB group) (double positive percentages of ILT3 and ILT4 in BL group) (double positive percentages of ILT3 and ILT4 in LL group) (double positive percentages of ILT3 and ILT4 in LL group). 2. The expression percentages of ILT3 and ILT4 on the surface of CD14 + monocytes from high to low were polybacterial in the order of culture. CD14+ monocytes in the peripheral blood of leprosy patients were much higher than those of normal persons. 2. The ratio of CD14+ monocytes in the peripheral blood of leprosy patients was much higher than that of normal persons. The expression of ILT3 and ILT4 in peripheral blood CD14 + monocytes was up-regulated by serum soluble factors in TT group BT group BB group BL group LL group 3. The order of expression percentage of ILT3 and ILT4 in cultured peripheral blood CD14 + monocytes from high to low was [MB] serum + normal human CD14. [PB] serum + normal CD14 + monocytes] [normal human serum + normal human CD14 + monocytes] [normal human serum + normal human CD14 + monocytes], so it can be explained that the soluble factors in leprosy patients serum do have the effect of up-regulating the expression of CD14 + monocyte surface ILT3, ILT4. 4. CD14 + monocyte surface ILT3, ILT4 expression percentage of MB group PB, and by This indicated that ILT3 and ILT4 had higher levels of leprosy and higher level of autoimmunity.
【学位授予单位】：昆明医科大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R755

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