Toll样受体2、4、9mRNA在带状疱疹患者外周血单个核细胞中的表达及意义
发布时间：2018-09-10 21:20
【摘要】： 目的研究带状疱疹患者外周血单个核细胞中Toll样受体(TLR) TLR2、4、9mRNA的表达情况,并探讨其与带状疱疹的发病关系。方法采用SYBER Green I实时定量PCR方法检测30例带状疱疹患者和20例正常人的外周血单一核细胞中TLR2、TLR4、TLR9mRNA的表达水平。结果带状疱疹患者与正常人对照,其外周血单一核细胞中TLR2、TLR4、TLR9mRNA的表达水平均明显升高,其中TLR2(t=2.23,P=0.031),TLR4(t=2.32,P=0.028),TLR9(t=2.27,P=0.031),具有统计学差异(P0.05)。结论带状疱疹患者外周血单一核细胞中TLR2、TLR4、TLR9mRNA的表达水平都明显升高,推测：在带状疱疹患者的发病过程中,TLR2、TLR4、TLR9mRNA可能参与了水痘-带状疱疹病毒的识别及抗病毒免疫。
[Abstract]:Objective to investigate the expression of Toll like receptor (TLR) TLR2,4,9mRNA in peripheral blood mononuclear cells of patients with herpes zoster and its relationship with herpes zoster. Methods the expression of TLR2,TLR4,TLR9mRNA in peripheral blood mononuclear cells (PBMC) of 30 herpes zoster patients and 20 normal controls was detected by SYBER Green I real time quantitative PCR. Results the expression of TLR2,TLR4,TLR9mRNA in peripheral blood mononuclear cells in patients with herpes zoster was significantly higher than that in normal controls. The expression of TLR4 (TLR4) was significantly higher in patients with herpes zoster than that in normal controls, and there was statistical difference (P0.05) in the expression of TLR9 (TLR9) in peripheral blood mononuclear cells of patients with herpes zoster and normal controls (P 0.05). Conclusion the expression of TLR2,TLR4,TLR9mRNA in peripheral blood mononuclear cells of patients with herpes zoster is significantly increased. It is speculated that TLR2TLR4 and TLR9 mRNA may be involved in the recognition of varicella-zoster virus and antiviral immunity in patients with herpes zoster.
【学位授予单位】：遵义医学院
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R752.12
本文编号：2235643
[Abstract]:Objective to investigate the expression of Toll like receptor (TLR) TLR2,4,9mRNA in peripheral blood mononuclear cells of patients with herpes zoster and its relationship with herpes zoster. Methods the expression of TLR2,TLR4,TLR9mRNA in peripheral blood mononuclear cells (PBMC) of 30 herpes zoster patients and 20 normal controls was detected by SYBER Green I real time quantitative PCR. Results the expression of TLR2,TLR4,TLR9mRNA in peripheral blood mononuclear cells in patients with herpes zoster was significantly higher than that in normal controls. The expression of TLR4 (TLR4) was significantly higher in patients with herpes zoster than that in normal controls, and there was statistical difference (P0.05) in the expression of TLR9 (TLR9) in peripheral blood mononuclear cells of patients with herpes zoster and normal controls (P 0.05). Conclusion the expression of TLR2,TLR4,TLR9mRNA in peripheral blood mononuclear cells of patients with herpes zoster is significantly increased. It is speculated that TLR2TLR4 and TLR9 mRNA may be involved in the recognition of varicella-zoster virus and antiviral immunity in patients with herpes zoster.
【学位授予单位】：遵义医学院
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R752.12
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