尖锐湿疣组织中p-STAT3和COX-2的表达及意义
发布时间：2018-09-17 08:15
【摘要】：背景和目的 尖锐湿疣(condylomata acuminatum,CA)是性传播疾病(sexually transmitted disease,STD)的一种,由人类乳头瘤病毒(human papillomavirus,HPV)感染生殖器、会阴和肛门等部位引起的一种常见的性传播疾病。HPV是双链DNA病毒,在鳞状上皮复制,通过与受损的皮肤接触进行传播。近年来,其发生率迅速增加,据我国性病检测报道,生殖器HPV检测率为30.69/10万,占我国性传播疾病的第二位；在国外,其发病率仅次于淋病,研究其发病机制对控制CA具有十分重要的意义。本实验通过对P-STAT3和COX-2在CA组织中的表达及其相关性的研究探讨尖锐湿疣的发病机制,为其预防和治疗提供理论依据。 尖锐湿疣具有增生快、易复发的特点,少数患者过度增生成为巨大型尖锐湿疣,部分可发生肿瘤。近年来研究发现在人体鳞状上皮细胞感染HPV后存在细胞凋亡和增殖异常,关于其机制的研究较多,但P-STAT3和COX-2在尖锐湿疣及生殖器癌的发病机制研究较少,本实验我们用免疫组织化学SP法分别检测43例CA组织和32例正常人包皮组织中P-STAT3和COX-2的表达,并分析CA组织中P-STAT3和COX-2表达的相关性。 材料和方法 1材料 43例CA患者均来自郑州大学第一附属医院皮肤性病科门诊,具有典型皮损,并经组织病理证实,其中男31例,女12例；年龄17-55(31.42±7.28)岁；病程20-108(54.7±13.63)天；以32例由泌尿外科手术切除的正常男性包皮作为对照。 2方法 应用免疫组化SP法分别检测43例CA组织和32例正常人包皮组织中p-STAT3和COX-2的表达,操作步骤均按试剂盒说明进行。P-STAT3阳性显色为细胞核内出现淡黄色至棕褐色颗粒；COX-2阳性显色为细胞膜和胞膜附近的胞质出现淡黄色至棕褐色颗粒。 3统计学处理 采用SPSS17.0进行分析,应用两独立样本秩和检验比较两组组织中P-STAT3和COX-2蛋白表达水平的差异,应用x2检验比较两组阳性表达率差异,应用Pearson列联系数方法分析CA组织中两种蛋白表达的关联性,检验水准a=0.05. 结果 1CA组织和正常上皮组织中P-STAT3蛋白的表达 P-STAT3阳性显色为细胞核内出现淡黄色至棕褐色颗粒。43例CA组织中有28例P-STAT3阳性表达,阳性表达率65.12%(28/43),表达强度多在++~+++,P-STAT3阳性细胞主要位于棘细胞层和颗粒层角质形成细胞的胞核中,正常上皮组织中无表达。两组阳性表达率差异有统计学意义(x2=30.53,P0.05),两组阳性表达强度差异有统计学意义(Z=5.575,P0.001)。 2CA组织和正常上皮组织中COX-2蛋白的表达 COX-2阳性显色为细胞膜和胞膜附近的胞质出现淡黄色至棕褐色颗粒。43例CA组织中有32例COX-2阳性表达,阳性表达率74.42%(32/43),表达强度多在++～+++,阳性细胞主要位于棘层的中上部和颗粒层。正常上皮组织中COX-2仅有少量表达,阳性表达率6.25%(2/32),表达强度在-~++,两组阳性表达率差异有统计学意义(x2=31.71,P0.05),两组阳性表达强度差异有统计学意义(Z=5.713,P0.001)。 3CA组织中p-STAT3和COX-2表达的关联性分析 在43例CA组织中p-STAT3表达阳性的有28例,15例阴性；有32例COX-2表达阳性,11例阴性。经Pearson相关分析后发现,43例CA组织中p-STAT3与COX-2表达呈正相关(r=0.577，P0.001)。 结论 1.CA组织中存在p-STAT3的过表达,p-STAT3的过表达可能与CA组织细胞的过度增殖有关； 2.CA组织中存在COX-2的过表达,提示COX-2对CA的发生发展起重要作用； 3.在CA组织中p-STAT3与COX-2的表达呈正相关,二者的共同作用促进CA的发生和发展。
[Abstract]:Background and purpose
Condylomata acuminatum (CA) is a sexually transmitted disease (STD), caused by human papillomavirus (HPV) infection of genitals, perineum and anus and other parts of a common sexually transmitted disease. Contact transmission. In recent years, the incidence of STD has increased rapidly. According to the reports of STD detection in China, the detection rate of HPV in genitals is 3069/100,000, accounting for the second place of STD in China. In foreign countries, the incidence of HPV is second only to gonorrhea, so it is very important to study its pathogenesis for controlling CA. To explore the pathogenesis of condyloma acuminatum and provide theoretical basis for its prevention and treatment.
Condyloma acuminatum (CA) has the characteristics of rapid proliferation and easy recurrence. Excessive hyperplasia in a few patients has become a giant CA, and some of them can develop tumors. In recent years, studies have found that there are abnormal apoptosis and proliferation in human squamous epithelial cells infected with HPV. There are many studies on its mechanism, but P-STAT3 and COX-2 in CA and genital cancer. In this study, we detected the expression of P-STAT3 and COX-2 in 43 CA tissues and 32 normal prepuce tissues by immunohistochemical SP method, and analyzed the correlation between the expression of P-STAT3 and COX-2 in CA tissues.
Materials and methods
1 material
43 patients with CA were all from the dermatology and venereal disease clinic of the First Affiliated Hospital of Zhengzhou University. The typical lesions were confirmed by histopathology, including 31 males and 12 females, aged 17-55 (31.42 (+ 7.28) years, 20-108 (54.7 (+ 13.63) days, and 32 normal male prepuce excised by urological surgery as control.
2 method
The expression of p-STAT3 and COX-2 in 43 CA tissues and 32 normal prepuce tissues were detected by immunohistochemical SP method. The operation procedure was carried out according to the kit instructions. Grain.
3 statistical processing
SPSS17.0 was used to analyze the expression of P-STAT3 and COX-2 protein in CA tissues. The expression of P-STAT3 and COX-2 protein in CA tissues was compared by rank sum test of two independent samples. The positive expression rate of P-STAT3 and COX-2 protein in CA tissues was compared by x2 test.
Result
Expression of P-STAT3 protein in 1CA and normal epithelial tissue
The positive staining of P-STAT3 was pale yellow to brown granules in the nucleus. 28 of 43 CA tissues were positive for P-STAT3. The positive expression rate was 65.12%(28/43). The expression intensity of P-STAT3 was mostly +++. The P-STAT3 positive cells were mainly located in the nuclei of keratinocytes in the spinous cell layer and granular layer, but not in the normal epithelium. The positive expression intensity of the two groups was significantly different (Z = 5.575, P 0.001).
Expression of COX-2 protein in 2CA and normal epithelial tissue
The positive staining of COX-2 was pale yellow to brown granules in the cytoplasm near the cell membrane and membrane. In 43 CA tissues, 32 cases were positive for COX-2, the positive expression rate was 74.42%(32/43). The expression intensity of COX-2 was mostly +++, and the positive cells were mainly located in the middle and upper part of the spinous layer and granular layer. The positive rate was 6.25% (2/32) and the expression intensity was -++. The difference was statistically significant between the two groups (x2 = 31.71, P 0.05). The difference was statistically significant between the two groups (Z = 5.713, P 0.001).
Correlation analysis of p-STAT3 and COX-2 expression in 3CA tissues
There were 28 positive and 15 negative cases of p-STAT3 expression in 43 CA tissues, 32 positive and 11 negative cases of COX-2 expression. Pearson correlation analysis showed that p-STAT3 expression was positively correlated with COX-2 expression in 43 CA tissues (r=0.577, P 0.001).
conclusion
1. The overexpression of p-STAT3 in CA tissues may be related to the overproliferation of CA cells.
The over expression of COX-2 in 2.CA tissues suggests that COX-2 plays an important role in the development of CA.
3. The expression of p-STAT3 and COX-2 was positively correlated in CA tissues. The co-action of p-STAT3 and COX-2 promoted the occurrence and development of CA.
【学位授予单位】：郑州大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R752.53
本文编号：2245272
[Abstract]:Background and purpose
Condylomata acuminatum (CA) is a sexually transmitted disease (STD), caused by human papillomavirus (HPV) infection of genitals, perineum and anus and other parts of a common sexually transmitted disease. Contact transmission. In recent years, the incidence of STD has increased rapidly. According to the reports of STD detection in China, the detection rate of HPV in genitals is 3069/100,000, accounting for the second place of STD in China. In foreign countries, the incidence of HPV is second only to gonorrhea, so it is very important to study its pathogenesis for controlling CA. To explore the pathogenesis of condyloma acuminatum and provide theoretical basis for its prevention and treatment.
Condyloma acuminatum (CA) has the characteristics of rapid proliferation and easy recurrence. Excessive hyperplasia in a few patients has become a giant CA, and some of them can develop tumors. In recent years, studies have found that there are abnormal apoptosis and proliferation in human squamous epithelial cells infected with HPV. There are many studies on its mechanism, but P-STAT3 and COX-2 in CA and genital cancer. In this study, we detected the expression of P-STAT3 and COX-2 in 43 CA tissues and 32 normal prepuce tissues by immunohistochemical SP method, and analyzed the correlation between the expression of P-STAT3 and COX-2 in CA tissues.
Materials and methods
1 material
43 patients with CA were all from the dermatology and venereal disease clinic of the First Affiliated Hospital of Zhengzhou University. The typical lesions were confirmed by histopathology, including 31 males and 12 females, aged 17-55 (31.42 (+ 7.28) years, 20-108 (54.7 (+ 13.63) days, and 32 normal male prepuce excised by urological surgery as control.
2 method
The expression of p-STAT3 and COX-2 in 43 CA tissues and 32 normal prepuce tissues were detected by immunohistochemical SP method. The operation procedure was carried out according to the kit instructions. Grain.
3 statistical processing
SPSS17.0 was used to analyze the expression of P-STAT3 and COX-2 protein in CA tissues. The expression of P-STAT3 and COX-2 protein in CA tissues was compared by rank sum test of two independent samples. The positive expression rate of P-STAT3 and COX-2 protein in CA tissues was compared by x2 test.
Result
Expression of P-STAT3 protein in 1CA and normal epithelial tissue
The positive staining of P-STAT3 was pale yellow to brown granules in the nucleus. 28 of 43 CA tissues were positive for P-STAT3. The positive expression rate was 65.12%(28/43). The expression intensity of P-STAT3 was mostly +++. The P-STAT3 positive cells were mainly located in the nuclei of keratinocytes in the spinous cell layer and granular layer, but not in the normal epithelium. The positive expression intensity of the two groups was significantly different (Z = 5.575, P 0.001).
Expression of COX-2 protein in 2CA and normal epithelial tissue
The positive staining of COX-2 was pale yellow to brown granules in the cytoplasm near the cell membrane and membrane. In 43 CA tissues, 32 cases were positive for COX-2, the positive expression rate was 74.42%(32/43). The expression intensity of COX-2 was mostly +++, and the positive cells were mainly located in the middle and upper part of the spinous layer and granular layer. The positive rate was 6.25% (2/32) and the expression intensity was -++. The difference was statistically significant between the two groups (x2 = 31.71, P 0.05). The difference was statistically significant between the two groups (Z = 5.713, P 0.001).
Correlation analysis of p-STAT3 and COX-2 expression in 3CA tissues
There were 28 positive and 15 negative cases of p-STAT3 expression in 43 CA tissues, 32 positive and 11 negative cases of COX-2 expression. Pearson correlation analysis showed that p-STAT3 expression was positively correlated with COX-2 expression in 43 CA tissues (r=0.577, P 0.001).
conclusion
1. The overexpression of p-STAT3 in CA tissues may be related to the overproliferation of CA cells.
The over expression of COX-2 in 2.CA tissues suggests that COX-2 plays an important role in the development of CA.
3. The expression of p-STAT3 and COX-2 was positively correlated in CA tissues. The co-action of p-STAT3 and COX-2 promoted the occurrence and development of CA.
【学位授予单位】：郑州大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R752.53
【参考文献】
相关期刊论文 前10条
1 刘俊茹;王原;左连富;李凤林;王莹;刘嘉琳;;COX-2、p-Stat3及p-Stat5在食管癌组织中的表达及其意义[J];癌症;2007年05期
2 王芳;王翠莲;郭丽丽;王志萍;;COX-2 Survivin在大肠癌组织中的表达及意义[J];长治医学院学报;2010年01期
3 李艳丽;杨丽丽;郑红兵;;COX-2在宫颈病变中的表达及其与高危型HPV感染的相关性[J];当代医学;2010年33期
4 郭华阳，周来新，刘斌，钟白玉，叶庆佾，谭骏，刘昕;复发尖锐湿疣中人乳头瘤病毒基因的原位杂交法分型观察[J];第三军医大学学报;1996年01期
5 刘彤;张洪辉;;尖锐湿疣患者病损中VEGF、COX-2及MVD的作用探讨[J];四川大学学报(医学版);2009年02期
6 佘晓玲;刘保安;;Stat3与肿瘤的侵袭转移[J];现代生物医学进展;2007年02期
7 郭颖;李海;陈殷;陈芳;张阳;;环氧化酶-2和血管内皮生长因子在尖锐湿疣组织中的表达及临床病理意义[J];实用医技杂志;2006年21期
8 王海清;王海琳;;磷酸化转录信号转导子及激活子3和细胞周期素D1的表达与宫颈癌临床病理特点的关系[J];实用医学杂志;2008年09期
9 冉凤明;魏万里;胡红艳;王小芳;;STAT3、p-STAT3、HIF-1α、VEGF蛋白表达与肺癌侵袭和转移的相关性研究[J];实用医学杂志;2011年14期
10 冯松涛;张志刚;;人乳头瘤病毒致癌机制的研究进展[J];国际病理科学与临床杂志;2010年01期
相关硕士学位论文 前1条
1 刘新;TRX与STAT-3在肝细胞肝癌高表达及相互作用研究[D];浙江大学;2007年
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