白细胞介素-18和33 mRNA在变应性接触性皮炎患者外周血中的表达及治疗后的变化

发布时间：2018-09-17 08:24

【摘要】：目的:探讨白细胞介素(IL)-18和IL-33 mRNA在变应性接触性皮炎患者与健康人外周血中的表达差异及其与湿疹皮损面积及严重度指数(eczema area and severity index,EASI)、部分实验室指标如白细胞、嗜酸性粒细胞及淋巴细胞计数的相关性;探讨应用白芍总苷治疗变应性接触性皮炎2周及4周上述细胞因子的变化。方法:采用实时荧光定量PCR方法,检测80例变应性接触性皮炎患者和40例健康对照者外周血IL-18、IL-33 mRNA的表达量;对患者应用白芍总苷(total glucosides of paenoy,TGP)治疗2周和4周后外周血IL-18 mRNA和IL-33 mRNA表达水平进行检测。结果:变应性接触性皮炎患者外周血IL-18和IL-33 mRNA的表达量明显高于正常对照组。急性期组、亚急性期组和慢性期组IL-18 mRNA和IL-33mRNA表达量均高于对照组,且上述3组中IL-18 mRNA和IL-33 mRNA表达量从高到低依次为:急性期组亚急性期组慢性期组。经白芍总苷治疗2周后,变应性接触性皮炎患者外周血IL-18和IL-33mRNA表达均较治疗前明显降低。白芍总苷治疗4周后变应性接触性皮炎患者外周血中IL-18和IL-33比治疗2周后明显降低。IL-18、IL-33ΔCT值与嗜酸性粒细胞数目呈负相关(r值分别为-0.56,-0.59,P均0.01)。有家族过敏史的变应性接触性皮炎患者组较无家族过敏史患者组IL-18和IL-33 mRNA表达量高。结论:IL-18和IL-33可能参与了变应性接触性皮炎的发病过程并与疾病分型和严重程度有关。白芍总苷可能通过调节变应性接触性皮炎患者IL-18和IL-33 mRNA表达量对变应性接触性皮炎起到治疗作用。
[Abstract]:Objective: to investigate the expression of interleukin (IL) 18 and IL-33 mRNA in peripheral blood of patients with allergic contact dermatitis and healthy subjects, and to investigate the differences with the area of eczema and severity index (eczema area and severity index,EASI), and some laboratory indexes such as white blood cells (WBC). The correlation between eosinophil and lymphocyte count and the changes of cytokines in allergic contact dermatitis treated with total glucosides of paeony were studied. Methods: the expression of IL-18,IL-33 mRNA in peripheral blood of 80 patients with allergic contact dermatitis and 40 healthy controls was detected by real-time fluorescence quantitative PCR. The expression of IL-18 mRNA and IL-33 mRNA in peripheral blood of patients treated with total glucoside of paeony (total glucosides of paenoy,TGP) for 2 weeks and 4 weeks were detected. Results: the expression of IL-18 and IL-33 mRNA in peripheral blood of patients with allergic contact dermatitis was significantly higher than that of normal controls. The expressions of IL-18 mRNA and IL-33mRNA in acute, subacute and chronic phase groups were higher than those in control group. The order of IL-18 mRNA and IL-33 mRNA expression in the above three groups was as follows: acute stage group, subacute phase group, chronic phase group. The expression of IL-18 and IL-33mRNA in peripheral blood of patients with allergic contact dermatitis was significantly lower than that before treatment after 2 weeks of treatment with total glucosides of paeony. The levels of IL-18 and IL-33 in peripheral blood of patients with allergic contact dermatitis after 4 weeks of total paeony glucoside treatment were significantly lower than those after 2 weeks treatment. The values of IL-18 and IL-33 螖 CT were negatively correlated with the number of eosinophilic granulocytes (r = -0.56 卤-0.59, P < 0.01, respectively). The expression of IL-18 and IL-33 mRNA in allergic contact dermatitis with family history was higher than that without family allergic history. ConclusionTwo-one-IL-18 and IL-33 may be involved in the pathogenesis of allergic contact dermatitis and may be related to the type and severity of the disease. Total glucosides of paeony may play a therapeutic role in allergic contact dermatitis by regulating the expression of IL-18 and IL-33 mRNA in patients with allergic contact dermatitis.
【作者单位】：天津市人民医院皮肤科;天津市第五中心医院皮肤科;天津市中医药研究院附属医院中医呼吸科;天津医科大学总医院检验科;
【基金】：天津市卫生行业重点攻关项目基金(12KG131)资助项目
【分类号】：R758.2

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