河北省手足口病伴脱甲症病原分子特征分析

发布时间：2018-09-19 17:33

【摘要】：目的了解河北省手足口病伴脱甲症病原的分子流行病学特征,为手足口病伴脱甲症的监测和预防控制提供科学依据。方法采用随机抽样方法选取张家口、承德、保定、石家庄和邢台市5个监测点,对监测点2014年1-9月发生的手足口病实验室诊断病例进行脱甲症回顾性调查。进行病毒分离,对分离株VP1区进行PCR扩增及序列测定,比较核苷酸同源性;构建系统进化树,进行基因变异分析。结果 1 741名手足口病患者中45例出现指(趾)甲脱落症状,脱甲率为2.58%。其他肠道病毒感染者脱甲罹患率为7.80%,HEV71和CoxA16感染者脱甲罹患率分别为0.12%和0.26%,差异有统计学意义(χ2=87.33,P0.05)。45例脱甲手足口病患者中,其他肠道病毒感染病例43例,占95.56%,HEV71感染率占2.22%,CoxA16感染率占2.22%,差异有统计学意义(χ2值分别为78.44和78.44,均P0.05)。CoxA6脱甲毒株与未脱甲分离株同源性为94.5%~98.8%,与近年来我国各地的分离株同源性高达94.6%~98.7%,与芬兰等地手足口病伴脱甲症分离株同源性为92.7%~98.6%。系统进化树显示,CoxA6分离株与国际标准株Gdula及山东1996年之前的分离株亲缘关系较远,而与近年来的分离株亲缘关系较近。2株ECHO30核苷酸同源性为99.1%,与G亚型分离株同源性高于其他亚型,为G亚型。ECHO30分离株HBTJS010、HBTJS055在同一分支,均属于G亚型,与我国各地2008年之前的分离株亲缘关系较远,而与2008年后的分离株亲缘关系较近。结论河北省引起手足口病伴脱甲症的主要病原是CoxA6。
[Abstract]:Objective to investigate the molecular epidemiological characteristics of hand, foot and mouth disease with dethyroidism in Hebei Province, and to provide scientific basis for surveillance and prevention and control of hand foot mouth disease with dethyroidism. Methods A retrospective survey of dethyroidism in laboratory diagnosis of hand-foot-mouth disease occurred from January to September 2014 was carried out in five monitoring sites of Zhangjiakou Chengde Baoding Shijiazhuang and Xingtai by random sampling. The virus was isolated, the VP1 region was amplified by PCR and sequenced, nucleotide homology was compared, and phylogenetic tree was constructed for gene mutation analysis. Results among the 1 741 patients with hand, foot and mouth disease, 45 cases had the symptoms of nail loss, and the dethyroidism rate was 2.58%. The incidence of de-armour in other enterovirus infections was 7.80% and 0.26% in HEV71 and CoxA16 infection, respectively. The difference was statistically significant (蠂 2 87.33 P 0.05) .There were 43 cases of other enterovirus infection in 45 patients with dethyroifying hand, foot and mouth disease. The infection rate of CoxA71 was 95.566.The infection rate of CoxA71 was 2.22%, and the difference was statistically significant (蠂 ~ 2 = 78.44 and 78.44, respectively, P0.05). The homology between CoxA6 de-armour virus strain and unde-armored isolate strain was 94.5- 98.80.The homology of CoxA6 virus strain with other isolates in China in recent years was 94.60.77.It was 94.67.7m with Finland and so on. The homology of the isolated strains of foot mouth disease with dethyroidism was 92.70.98. 6%. Phylogenetic tree showed that CoxA6 isolates were closely related to the international standard Gdula and Shandong isolates before 1996. The nucleotide homology of ECHO30 was 99.1, and the nucleotide homology with G subtype was higher than that of other subtypes, indicating that G subtype. ECHO30 isolate HBTJS010,HBTJS055 belonged to G subtype in the same branch. The phylogenetic relationship between the isolates before 2008 and after 2008 was far away, and close to that of the isolates after 2008. Conclusion CoxA6. is the main pathogen of hand, foot and mouth disease with dethyroidism in Hebei Province.
【作者单位】：河北省疾病预防控制中心;
【基金】：河北省重大医学科研资助项目(No.zd2013068) 病毒性传染病病原谱流行规律及变异研究项目(No 2013ZX10004202) 河北省自然科学基金项目(No.C2010001553)
【分类号】：R725.1;R758.72

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