miR-199a抑制低氧诱导因子在银屑病患者皮损中的表达

发布时间：2018-10-10 14:39

【摘要】：目的探索miR-199a,HIF-1α在银屑病中的表达及其对表皮细胞增殖的影响。方法首先检测了27例寻常性银屑病患者皮损区和19例健康志愿者皮肤的miR-199a,HIF-1α的表达差异,分析二者之间的相关性;然后在Ha Ca T细胞中转染miR-199a mimics,用Western blot检测HIF-1α的蛋白表达水平;用双荧光素酶报告基因实验验证miR-199a与HIF-1α的靶向结合关系;最后用MTT法检测miR-199a,HIF-1α对Ha Ca T细胞增殖的影响。结果银屑病组织中miR-199a的表达下调,HIF-1α的表达上调,且两者存在负相关性;在Ha Ca T细胞中转染miR-199a mimics可下调HIF-1α的表达;HIF-1α可回复miR-199a对Ha Ca T细胞增殖的抑制作用。结论 miR-199a靶向HIF-1α调控表皮细胞的增殖从而参与银屑病的发病机制。
[Abstract]:Objective to investigate the expression of miR-199a,HIF-1 伪 in psoriasis and its effect on the proliferation of epidermal cells. Methods the expression of miR-199a,HIF-1 伪 in the skin of 27 patients with psoriasis vulgaris and 19 healthy volunteers was detected firstly, and the correlation between them was analyzed, then the expression of HIF-1 伪 was detected by Western blot in Ha Ca T cells transfected with miR-199a mimics, and the expression of HIF-1 伪 was detected by Western blot in the skin of 27 patients with psoriasis vulgaris and 19 healthy volunteers. Double luciferase reporter gene experiment was used to verify the targeting binding relationship between miR-199a and HIF-1 伪. Finally, the effect of miR-199a,HIF-1 伪 on the proliferation of Ha Ca T cells was detected by MTT assay. Results the expression of miR-199a was down-regulated and the expression of HIF-1 伪 was up-regulated in psoriatic tissues. The expression of HIF-1 伪 was down-regulated by transfection of miR-199a mimics in Ha Ca T cells, and the inhibitory effect of miR-199a on the proliferation of Ha Ca T cells was reversed by HIF-1 伪. Conclusion miR-199a targeting HIF-1 伪 regulates the proliferation of epidermal cells and participates in the pathogenesis of psoriasis.
【作者单位】：湖南中医药大学第二附属医院皮肤科;
【基金】：国家自然科学基金课题(81573985;81273769) 湖南省自然科学基金课题(No.2015JJ6081) 湖南省中医药科研计划(No.201467)
【分类号】：R758.63

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