寻常型白癜风血清T细胞亚群、可溶性白介素-2受体、肿瘤坏死因子-α与中医辨证的分析
发布时间：2018-10-16 19:51
【摘要】：研究目的： 1.以正常人为对照,观测寻常型进展期白癜风患者外周血中CD3、CD4、CD8、CD4/CD8及sIL-2R、TNF-α水平,探讨白癜风发病的细胞免疫机制。 2.分析白癜风中医辨证分型与外周血T细胞亚群及sIL-2R、TNF-α水平的相关性,探讨其中的规律性,有利于指导白癜风的临床分型诊治。 研究对象： 2011年6月至2012年3月期间至江苏省中医院皮肤科就诊的白癜风初诊患者,年龄在18-65周岁之间,共收集患者35例。诊断与分型分期依据《白癜风临床分型及疗效标准(2010年修订稿)》。这些患者近3个月内未系统服用过糖皮质激素、免疫抑制剂及免疫调节剂,之前未确诊过其他自身免疫性疾病。 方法： 1.试验组：35例(男性20例,女性15例)年龄从18到58周岁(33.63±10.09岁)的寻常型进展期白癜风患者。对照组：20例(男性10例,女性10例)年龄从23到64周岁(35.26±11.12岁)的健康个体,没有任何的自身免疫性疾病史。 2.采用统一设计的成人白癜风临床调查表并对患者本人询问,内容包括姓名、性别、年龄、病程、症状、体征、临床分型、分期、舌苔、脉象等。 3.检测方法：CD3、CD4、CD8采用流式细胞术检测,sIL-2R及TNF-α采用酶联免疫吸附法检测。 4.统计学方法：Excel软件建立数据库,SPSS19.O软件进行统计分析。统计采用独立样本t检验、秩和检验和X2检验,0.01P0.05为差异具有显著的统计学意义,P0.01为差异具有非常显著的统计学意义。 结果： 1.寻常型进展期白癜风患者的年龄构成在18-30岁,30-40岁,40-65岁之间未见差异,性别构成也无差异(P0.05)。 2.中医证型的分布与寻常型进展期白癜风不同亚型、年龄组及性别的构成无明显相关(P均0.05)。 3.与正常对照组相比较,寻常型进展期白癜风患者外周血CD3水平降低,有显著差异(0.01P0.05)；外周血CD4水平降低,有非常显著的差异(P0.01)；血CD8及CD4/CD8水平没有明显的统计学差异(P均0.05)。与正常对照相比,散在型进展期白癜风患者外周血CD3降低,有显著差异(0.01P0.05)；CD4降低,有非常显著的差异(P0.01)；CD8及CD4/CD8水平没有显著的统计学差异(P均0.05)。局限型进展期白癜风患者外周血CD3、CD4、CD8及CD4/CD8水平与正常对照组相比均没有显著的统计学差异(P均0.05)。 4.与正常对照组相比较,寻常型进展期白癜风患者组外周血sIL-2R水平升高,有非常显著的统计学差异(P0.01)；外周血TNF-α水平升高,有显著的统计学差异(0.01P0.05)。散在型患者组外周血sIL-2R及TNF-α水平与正常对照组比较均有非常显著的统计学差异(P均0.01)。局限型患者外周血sIL-2R、TNF-α水平与对照组相比,均没有显著的统计学差异(P均0.05)。 5.肝肾阴虚证、气滞血瘀证、湿热蕴结证患者组之间CD3、CD4、CD8、CD4/CD8、sIL-2R、及TNF-α水平均无显著统计学差异(P均0.05)。 结论： 1.本组寻常型进展期白癜风患者细胞免疫功能异常,主要表现为外周血CD3、CD4水平较正常对照组明显降低,而CD8及CD4/CD8水平与对照组相比却没有统计学差异,外周血sIL-2R、TNF-α水平较正常对照组明显增高。与正常对照组相比,散在型进展期白癜风患者外周血CD3、CD4水平明显降低,sIL-2R、TNF-α水平明显增高。而局限型与对照组相比却没有统计学差异。 2.寻常型进展期白癜风患者细胞免疫功能异常,提示了其发病与自身免疫有关,也进一步支持了白癜风的自身免疫发病学说。 3.白癜风各中医证型间外周血CD3、CD4、CD8、CD4/CD8、sIL-2R及TNF-α水平均没有明显统计学差异,提示了中医治疗白癜风的辨证分型物质基础是多方面的,与细胞免疫之间的关系需进一步探讨。
[Abstract]:Purpose of the study: 1. To observe the levels of CD3, CD4, CD8, CD4/ CD8 and sIL-2R, TNF-and CD4/ CD8 and sIL-2R, TNF-and in peripheral blood of patients with vitiligo in normal control and observe the cellular immunity of vitiligo. 2. To analyze the correlation between TCM syndrome differentiation typing and peripheral blood T cell subpopulations and sIL-2R, TNF levels in patients with vitiligo. diagnosis and treatment of bed type Study object: From June 2011 to March 2012, the patients with vitiligo in the dermatological department of Chinese Hospital of Jiangsu Province, aged 18-65 years old A total of 35 patients were collected. The diagnosis and classification stage was based on the clinical classification of vitiligo and the standard of efficacy. (Revised 2010)>. None of these patients received corticosteroids, immunosuppressive agents and immunomodulators for nearly 3 months before Have a visit to it Method: 1. Test group: 35 cases (male 20 cases, female 15 cases) from 18 to 58 years old (33. 63) 10 Control group: 20 patients (10 males and 10 females) with age from 23 to 64 years old (35. 26 vs. 11. 12 years old) Kang individual, without any autoimmune disease history. 2. Adopt the unified design adult vitiligo clinical investigation questionnaire and ask the patient oneself, the content includes name, sex, age, course of course, Symptoms, signs, clinical classification, stage, tongue coating, tongue coating, etc. 3. Detection methods: CD3, CD4, CD8 were detected by flow cytometry. sIL-2R and TNF-1R were detected by enzyme-linked immunosorbent assay. 4. Statistical methods: Exc The statistical analysis of the database and SPSS19. O software was carried out by the el software. The independent sample t test, the rank sum test and the X2 test were adopted, and the difference between 0. 01P0. 05 was significant. Statistics The significance, P0. 01 were significant statistical significance for the difference. Results: 1. The age of patients with vitiligo in the normal progression period was 18-30 years, 30-There was no difference between 40 and 65 years of age, and there was no difference in gender composition (P0.05). There was no significant correlation between the different subtypes, age groups and sex (P <0.05). 3. Compared with the control group, the level of CD3 in peripheral blood of patients with vitiligo vulgaris was decreased significantly (P0.05), and the level of CD4 in peripheral blood was decreased, and there were very significant differences (P There was no significant difference in the levels of CD8 and CD4/ CD8 in patients with vitiligo (P <0.05). Compared with normal controls, the CD3 decreased in peripheral blood of patients with vitiligo, and there were significant differences (0. 01P0. 05); CD4 decreased, and there was very significant difference (P <0.05). There was no significant statistical difference between CD8 and CD4/ CD8 levels (P 0.05). CD3, CD4, CD in peripheral blood of patients with vitiligo Compared with the control group, the levels of sIL-2R in peripheral blood of patients with vitiligo vulgaris were significantly higher than those of normal control group (P <0.05). (P 0.01), the level of TNF-VEP in peripheral blood increased, and there were significant statistical differences (0. 01P0. 05). The levels of IL-2R and TNF-IL were significantly different from those of normal control group (P 0.01). Compared with the control group, there was no significant difference in serum sIL-2R and TNF level (P 0.05). CD8 There was no significant difference in the levels of CD4/ CD8, sIL-2R and TNF-tau (P <0.05). Conclusion: 1. The abnormal cellular immune function of patients with vitiligo vulgaris in this group is mainly characterized by peripheral blood CD3, CD4 water. Compared with the normal control group, the level of CD8 and C was significantly lower than that in the control group. Compared with the control group, the levels of sIL-2R and TNF levels in peripheral blood were significantly higher than those in the control group. Compared with the group, the peripheral blood C of patients with vitiligo in the stage of progressive vitiligo The levels of CD3 and CD4 decreased significantly, and the levels of sIL-2R and TNF-2R were significantly higher, but there was no statistical difference compared with the control group. The abnormal cellular immune function of vitiligo patients with ordinary progression suggested that the pathogenesis of vitiligo is related to autoimmune diseases, and it also supports the theory of autoimmune disease of vitiligo. The peripheral blood CD3, CD4, CD8, CD4/ CD8, sIL-2R and TNF in each TCM syndrome type of vitiligo.
【学位授予单位】：南京中医药大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R758.41
本文编号：2275460
[Abstract]:Purpose of the study: 1. To observe the levels of CD3, CD4, CD8, CD4/ CD8 and sIL-2R, TNF-and CD4/ CD8 and sIL-2R, TNF-and in peripheral blood of patients with vitiligo in normal control and observe the cellular immunity of vitiligo. 2. To analyze the correlation between TCM syndrome differentiation typing and peripheral blood T cell subpopulations and sIL-2R, TNF levels in patients with vitiligo. diagnosis and treatment of bed type Study object: From June 2011 to March 2012, the patients with vitiligo in the dermatological department of Chinese Hospital of Jiangsu Province, aged 18-65 years old A total of 35 patients were collected. The diagnosis and classification stage was based on the clinical classification of vitiligo and the standard of efficacy. (Revised 2010)>. None of these patients received corticosteroids, immunosuppressive agents and immunomodulators for nearly 3 months before Have a visit to it Method: 1. Test group: 35 cases (male 20 cases, female 15 cases) from 18 to 58 years old (33. 63) 10 Control group: 20 patients (10 males and 10 females) with age from 23 to 64 years old (35. 26 vs. 11. 12 years old) Kang individual, without any autoimmune disease history. 2. Adopt the unified design adult vitiligo clinical investigation questionnaire and ask the patient oneself, the content includes name, sex, age, course of course, Symptoms, signs, clinical classification, stage, tongue coating, tongue coating, etc. 3. Detection methods: CD3, CD4, CD8 were detected by flow cytometry. sIL-2R and TNF-1R were detected by enzyme-linked immunosorbent assay. 4. Statistical methods: Exc The statistical analysis of the database and SPSS19. O software was carried out by the el software. The independent sample t test, the rank sum test and the X2 test were adopted, and the difference between 0. 01P0. 05 was significant. Statistics The significance, P0. 01 were significant statistical significance for the difference. Results: 1. The age of patients with vitiligo in the normal progression period was 18-30 years, 30-There was no difference between 40 and 65 years of age, and there was no difference in gender composition (P0.05). There was no significant correlation between the different subtypes, age groups and sex (P <0.05). 3. Compared with the control group, the level of CD3 in peripheral blood of patients with vitiligo vulgaris was decreased significantly (P0.05), and the level of CD4 in peripheral blood was decreased, and there were very significant differences (P There was no significant difference in the levels of CD8 and CD4/ CD8 in patients with vitiligo (P <0.05). Compared with normal controls, the CD3 decreased in peripheral blood of patients with vitiligo, and there were significant differences (0. 01P0. 05); CD4 decreased, and there was very significant difference (P <0.05). There was no significant statistical difference between CD8 and CD4/ CD8 levels (P 0.05). CD3, CD4, CD in peripheral blood of patients with vitiligo Compared with the control group, the levels of sIL-2R in peripheral blood of patients with vitiligo vulgaris were significantly higher than those of normal control group (P <0.05). (P 0.01), the level of TNF-VEP in peripheral blood increased, and there were significant statistical differences (0. 01P0. 05). The levels of IL-2R and TNF-IL were significantly different from those of normal control group (P 0.01). Compared with the control group, there was no significant difference in serum sIL-2R and TNF level (P 0.05). CD8 There was no significant difference in the levels of CD4/ CD8, sIL-2R and TNF-tau (P <0.05). Conclusion: 1. The abnormal cellular immune function of patients with vitiligo vulgaris in this group is mainly characterized by peripheral blood CD3, CD4 water. Compared with the normal control group, the level of CD8 and C was significantly lower than that in the control group. Compared with the control group, the levels of sIL-2R and TNF levels in peripheral blood were significantly higher than those in the control group. Compared with the group, the peripheral blood C of patients with vitiligo in the stage of progressive vitiligo The levels of CD3 and CD4 decreased significantly, and the levels of sIL-2R and TNF-2R were significantly higher, but there was no statistical difference compared with the control group. The abnormal cellular immune function of vitiligo patients with ordinary progression suggested that the pathogenesis of vitiligo is related to autoimmune diseases, and it also supports the theory of autoimmune disease of vitiligo. The peripheral blood CD3, CD4, CD8, CD4/ CD8, sIL-2R and TNF in each TCM syndrome type of vitiligo.
【学位授予单位】：南京中医药大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R758.41
【参考文献】
相关期刊论文 前10条
1 解春桃;;白癜风患者治疗前后血清IL-2、SIL-2R、TNF-α和外周血B细胞及T淋巴细胞亚群检测的临床意义[J];放射免疫学杂志;2007年05期
2 汪京峡;段爱霞;张大军;;白癜风患者血清中白介素及肿瘤坏死因子水平变化的研究[J];皮肤病与性病;2006年01期
3 张学玲;毛东军;刘影;葛邦友;沈景刚;沈奕;周彦;;白癜风患者心理健康水平的评估及治疗与细胞免疫功能的相关性研究[J];齐齐哈尔医学院学报;2010年14期
4 高天文,李强,李春英,李志强,王秋枫;白癜风2008例临床分析[J];中国皮肤性病学杂志;2002年05期
5 缪泽群,刘少金;寻常型白癜风患者外周血儿茶酚胺类物质水平的研究[J];中国皮肤性病学杂志;2004年03期
6 李东宁;裴洪菊;周和清;葛明康;董淑凤;张韵莉;孙健;杨素华;;白癜风患者外周血T细胞亚群的检测[J];中国皮肤性病学杂志;2006年07期
7 姜昱;雷立清;叶楠;周颖;张艳彩;;白癜风患者外周血CD_4,CD_8检测及免疫调节剂对其影响[J];中国皮肤性病学杂志;2009年05期
8 季素珍,施惠娟,沈丽玉;白癜风患者血清中可溶性白介素2受体的检测[J];中国皮肤性病学杂志;1999年01期
9 顾劲松,涂彩霞,谭雪晶,谷玲,林熙然;白癜风患者血清白介素、肿瘤坏死因子α及粒细胞-巨噬细胞集落刺激因子检测[J];中华皮肤科杂志;2003年02期
10 杨静,盛晚香;细胞间黏附分子1和白介素8与白癜风的相关性研究[J];中华皮肤科杂志;2003年11期
相关会议论文 前1条
1 卢良君;许爱娥;陈梅花;;白癜风分型分期与中医证型的关系[A];浙江省中西医结合学会皮肤科专业委员会第九次学术年会暨省级继教班资料汇编[C];2005年
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