角蛋白17在增殖性皮肤病中的表达

发布时间：2018-10-22 13:19

【摘要】：目的：研究角蛋白17(K17)在银屑病、脂溢性角化症、基底细胞癌、鳞状细胞癌、恶性黑色素瘤等增殖性皮肤病中的不同表达特点、病理意义和诊断,鉴别诊断中的应用价值。方法：通过免疫组化或免疫荧光法检测K17在正常皮肤、银屑病、脂溢性角化症、基底细胞癌、鳞状细胞癌、恶性黑色素瘤中的表达状况。结果：在正常皮肤,基底层、棘层、颗粒层的胞浆K17呈阴性表达,然而,在银屑病K17强表达,明显高于正常皮肤,表达于整个棘层,在基底层呈弱阳性或阴性表达,角化不全细胞不表达。在脂溢性角化症皮肤,K17表达在基底层和基底上层细胞,棘细胞上层及角质层不表达。在基癌组织中,瘤细胞不表达K17,瘤细胞旁的棘细胞层、颗粒层呈阳性表达。在鳞癌组织中,K17集中表达在高分化的鳞癌细胞中,在低分化的鳞癌细胞中不表达。在黑色素瘤中,K17强烈表达于表皮全层,但是,在瘤细胞和黑色素细胞中K17不表达。结论： K17的表达状况可为增殖性皮肤病诊断提供临床病理学方面的依据。
[Abstract]:Objective: to study the expression of keratin 17 (K17) in psoriasis, seborrheic keratosis, basal cell carcinoma, squamous cell carcinoma, malignant melanoma and other proliferative dermatosis. Application value of differential diagnosis. Methods: the expression of K17 in normal skin, psoriasis, seborrheic keratosis, basal cell carcinoma, squamous cell carcinoma and malignant melanoma was detected by immunohistochemistry or immunofluorescence. Results: cytoplasmic K17 expression was negative in normal skin, basal layer, spinous layer and granular layer. However, strong expression of K17 in psoriasis was significantly higher than that in normal skin, which was expressed in the whole spinous layer, and was weakly positive or negative in basal layer. Keratosis is not expressed in whole cells. In seborrheic keratosis skin, K17 was expressed in basal layer and basal layer, but not in spinous cell and keratinocyte. In basal carcinoma, K17 was not expressed in the tumor cells, but in the spinous cell layer next to the tumor cell, and positive expression was found in the granular layer. K17 was expressed mainly in highly differentiated squamous cell carcinoma cells and not in poorly differentiated squamous cell carcinoma cells. In melanoma, K17 was strongly expressed in the whole epidermis, but not in tumor cells and melanocytes. Conclusion: the expression of K 17 may provide clinicopathological basis for the diagnosis of proliferative dermatosis.
【学位授予单位】：浙江大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R751

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