Toll样受体4、9和C型凝集素受体在尖锐湿疣患者皮损的表达及其临床病理意义

发布时间：2018-10-22 13:37

【摘要】：目的探讨初发和复发尖锐湿疣(CA)患者皮损中TLR4、TLR9和DC-SIGN的表达及其临床病理意义。方法收集正常人皮肤（包皮）20例、初发CA组织30例、复发CA组织30例，，采用免疫组织化学链霉抗生物素蛋白－过氧化物酶法（SP法）检测以上组织中TLR4、TLR9和DC-SIGN的表达及分布。参照Fromowitz评分标准得出每个标本结果，应用SPSS17．0统计软件以t检验法比较TLR4、TLR9和DC-SIGN在初发CA皮损与复发CA皮损中的差异，Spearman等级相关分析TLR4、TLR9和DC-SIGN在初发CA皮损与复发CA皮损中的的相关性。结果TLR4、TLR9和DC-SIGN在初发CA皮损和复发CA皮损中的阳性率均为100%，在正常包皮组织中阳性率分别为5%、10%、5%。初发CA皮损和复发CA皮损中TLR4、TLR9和DC-SIGN的表达水平均显著高于正常包皮组织（P0.001），且阳性细胞集中分布于基底层和棘层。TLR4、TLR9和DC-SIGN三者在初发CA皮损和复发CA皮损中的表达差异均无统计学意义（三者均P0.05）。TLR4、TLR9与DC-SIGN在初发CA皮损和复发CA皮损中的表达均呈正相关性关系(rs1=0.489，P0.05;rs2=0.475，P0.05)。结论TLR4、TLR9和DC-SIGN在初发CA皮损和复发CA皮损中的表达明显增强可能在CA的发病和复发过程中起到一定作用。
[Abstract]:Objective to investigate the expression and clinicopathological significance of TLR4,TLR9 and DC-SIGN in the lesions of patients with primary and recurrent condyloma acuminatum (CA). Methods 20 cases of normal skin (prepuce), 30 cases of primary CA and 30 cases of recurrent CA were collected. The expression and distribution of TLR4,TLR9 and DC-SIGN in the tissues were detected by immunohistochemical streptavidin-peroxidase (SP) method. The results of each specimen were obtained by reference to the Fromowitz score, SPSS17.0 statistical software was used to compare the difference between TLR4,TLR9 and DC-SIGN in primary CA lesions and recurrent CA lesions. Spearman grade correlation was used to analyze the correlation between TLR4,TLR9 and DC-SIGN in primary CA lesions and recurrent CA lesions. Results the positive rates of TLR4,TLR9 and DC-SIGN in the primary CA lesions and recurrent CA lesions were 100, and in the normal prepuce tissues, the positive rates were 51010 and 555, respectively. The expression levels of TLR4,TLR9 and DC-SIGN in primary CA lesions and recurrent CA lesions were significantly higher than those in normal prepuce tissues (P0.001), and the positive cells were concentrated in the basal layer and spinous layer. The expression of TLR4,TLR9 and DC-SIGN in the primary CA lesions and recurrent CA lesions was poor. The expression of TLR4,TLR9 and DC-SIGN in primary CA lesions and recurrent CA lesions were positively correlated (rs1=0.489,P0.05;rs2=0.475,P0.05). Conclusion the increased expression of TLR4,TLR9 and DC-SIGN in primary CA lesions and recurrent CA lesions may play a role in the pathogenesis and recurrence of CA.
【学位授予单位】：广州医学院
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R752.53

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