Cyr61上调人角质形成细胞系表达趋化因子与银屑病发生机制初探
发布时间：2018-11-03 16:03
【摘要】：银屑病是一种常见的慢性炎症性皮肤疾病,多种炎症细胞与炎症因子参与其发病。我们已有研究发现促炎因子Cyr61/CCN1通过活化角质细胞而加剧皮损,但是否还有其他促炎机制尚不清楚。本研究通过Cyr61介导角质细胞产生趋化因子的体外实验,探讨Cyr61参与银屑病发生新途径。采用Real-time PCR方法检测人角质形成细胞系(HaCat细胞)在外源性Cyr61作用下趋化因子的表达格局。实验结果显示Cyr61能够在体外促进人角质形成细胞系表达多种趋化因子(如CCL2,CCL17,CCL22,CXCL1,CXCL10,CXCL11(P0.05)),提示Cyr61可能通过促进角质形成细胞系表达多种趋化因子从而参与DC,Th17,Th1等细胞的趋化。提示Cyr61可能通过促进角质形成细胞系表达趋化因子介导炎性细胞局部浸润增多,加剧银屑病发病。
[Abstract]:Psoriasis is a common chronic inflammatory skin disease. Many inflammatory cells and inflammatory factors are involved in the pathogenesis of psoriasis. We have found that Cyr61/CCN1 exacerbates skin lesions by activating keratinocytes, but it is not clear whether there are other inflammatory mechanisms. In this study, Cyr61 mediated keratinocytes to produce chemokines in vitro to explore the role of Cyr61 in the pathogenesis of psoriasis. The expression pattern of chemokines in human keratinocytes (HaCat cells) induced by exogenous Cyr61 was detected by Real-time PCR method. The results show that Cyr61 can promote the expression of many chemokines (such as CCL2,CCL17,CCL22,CXCL1,CXCL10,CXCL11 (P0.05),) in human keratinocytes in vitro. These results suggest that Cyr61 may be involved in the chemotaxis of DC,Th17,Th1 and other cells by promoting the expression of many chemokines in keratinocytes. It is suggested that Cyr61 may increase the local infiltration of inflammatory cells by promoting the expression of chemokines in keratinocytes and aggravate the pathogenesis of psoriasis.
【作者单位】： 上海交通大学医学院附属第九人民医院皮肤科;上海交通大学医学院上海市免疫学研究所;
【基金】：上海市科技委员会重点项目(13JC1402300)
【分类号】：R758.63
[Abstract]:Psoriasis is a common chronic inflammatory skin disease. Many inflammatory cells and inflammatory factors are involved in the pathogenesis of psoriasis. We have found that Cyr61/CCN1 exacerbates skin lesions by activating keratinocytes, but it is not clear whether there are other inflammatory mechanisms. In this study, Cyr61 mediated keratinocytes to produce chemokines in vitro to explore the role of Cyr61 in the pathogenesis of psoriasis. The expression pattern of chemokines in human keratinocytes (HaCat cells) induced by exogenous Cyr61 was detected by Real-time PCR method. The results show that Cyr61 can promote the expression of many chemokines (such as CCL2,CCL17,CCL22,CXCL1,CXCL10,CXCL11 (P0.05),) in human keratinocytes in vitro. These results suggest that Cyr61 may be involved in the chemotaxis of DC,Th17,Th1 and other cells by promoting the expression of many chemokines in keratinocytes. It is suggested that Cyr61 may increase the local infiltration of inflammatory cells by promoting the expression of chemokines in keratinocytes and aggravate the pathogenesis of psoriasis.
【作者单位】： 上海交通大学医学院附属第九人民医院皮肤科;上海交通大学医学院上海市免疫学研究所;
【基金】：上海市科技委员会重点项目(13JC1402300)
【分类号】：R758.63
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