湿疹患者外周血白介素17和白介素23水平及白芍总苷治疗前后的变化

发布时间：2018-11-11 11:00

【摘要】：目的:探讨细胞因子白介素(IL)17和IL-23在湿疹发病机制中的可能作用和临床意义。方法:运用实时荧光定量反转录聚合酶链式反应(RT-PCR),检测80例湿疹患者和40例正常人外周血IL-17和IL-23 mRNA表达的差异以及与临床、实验室指标的相关性。采用固相夹心法酶联免疫吸附实验(ELISA)研究在45例湿疹患者和30名正常人外周血中IL-17和IL-23蛋白的表达及其差异,初步探讨IL-17和IL-23在湿疹发病机制中的作用和临床意义。以及不同中医分型湿疹患者和白芍总苷治疗湿疹前后IL-17和IL-23表达的差异。结果:湿疹患者外周血中IL-17和IL-23 mRNA水平明显高于正常组(P0.05),且与湿疹面积及严重度指数(EASI)评分呈正相关。急性期和亚急性期湿疹患者血清中IL-17和IL-23水平明显高于慢性期患者和正常组(P0.05),湿疹患者血清中IL-17与IL-23表达水平呈正直线相关(r=0.53,P0.05)关系。白芍组患者治疗后IL-17和IL-23 mRNA水平,EASI及皮肤病生活质量指数量表(DLQI)下降程度较非白芍组大,且差异有统计学意义。结论:IL-17和IL-23可能参与了湿疹发病的病理过程。白芍总苷对于参与湿疹发病的细胞因子具有调节作用,可以用于湿疹的治疗。
[Abstract]:Objective: to investigate the possible role and clinical significance of cytokines (IL) 17 and IL-23 in the pathogenesis of eczema. Methods: real time quantitative reverse transcriptase chain reaction (RT-PCR) was used to detect the expression of IL-17 and IL-23 mRNA in peripheral blood of 80 eczema patients and 40 normal controls. The expression and difference of IL-17 and IL-23 proteins in peripheral blood of 45 eczema patients and 30 normal controls were studied by solid phase sandwich enzyme-linked immunosorbent assay (ELISA). To explore the role and clinical significance of IL-17 and IL-23 in the pathogenesis of eczema. The expression of IL-17 and IL-23 in patients with eczema of different TCM types and before and after treatment with total glucoside of paeony. Results: the levels of IL-17 and IL-23 mRNA in the peripheral blood of patients with eczema were significantly higher than those in the normal group (P0.05) and were positively correlated with the area of eczema and the (EASI) score of severity index. The serum levels of IL-17 and IL-23 in patients with eczema at acute and subacute stage were significantly higher than those in patients with chronic eczema and normal controls (P0.05). There was a positive linear correlation between the expression of IL-17 and IL-23 in patients with eczema (r = 0.53, P < 0.05). P0.05) relationship. After treatment, the levels of IL-17 and IL-23 mRNA, EASI and (DLQI) of skin disease quality of life in paeoniae Alba group were significantly lower than those in non-paeony group, and the difference was statistically significant. Conclusion: IL-17 and IL-23 may be involved in the pathogenesis of eczema. Total glucosides of paeony can regulate cytokines involved in eczema and can be used in the treatment of eczema.
【作者单位】：天津市中医药研究院附属医院皮肤科;天津市人民医院皮肤科;天津医科大学总医院检验科;
【基金】：中华医学会皮肤科学会科研基金天津市卫生行业重点攻关项目基金(12KG131)资助
【分类号】：R758.23

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