麻风和HIV合并感染者IL-10、IL-12、CCR5、CXCR4表达研究
发布时间：2018-11-15 19:34
【摘要】：目的：初步探讨麻风病和HIV合并感染后机体免疫状态的变化,为进一步做好麻风病和艾滋病的防治工作提供科学的依据。方法：收集麻风和HIV合并感染者3例、HIV感染者9例、麻风感染者10例、健康对照9例,采用流式细胞术检测CD4+和CD8+T细胞表面趋化因子受体CCR5和CXCR4表达情况；双抗体夹心ELISA方法检测血浆细胞因子IL-10、IL-12的含量；实时荧光定量PCR(RT-PCR)方法检测HIV病毒载量。结果：(1)麻风和HIV合并感染组、HIV感染组CD4+T细胞绝对计数和CD4/CD8比值均显著降低(p0.05),与麻风和HIV合并感染组对比,HIV感染组CD4/CD8比值显著降低(p0.05),进一步对HIV感染组分层后分析无统计学意义(p0.05)。(2)麻风和HIV合并感染组、HIV感染组CD4+T细胞表面的CCR5表达轻度升高,CD8+T细胞表面的CCR5表达轻度降低,但均无统计学意义(p0.05)；HIV感染组CD4+T细胞表面CCR5表达与病毒载量无相关性(r=0.119,p0.05)。(3)麻风和HIV合并感染组、麻风感染组、HIV感染组、健康对照组CD4+T细胞表面趋化因子受体CXCR4的表达无显著性差异(p0.05)。(4)麻风和HIV合并感染组、HIV感染组、麻风感染组IL-10表达水平均升高,但是与健康对照组比较无统计学意义(p0.05)；HIV感染组血浆IL-10与CD4+T细胞CCR5表达呈正相关(r=0.762p=0.028)。(5)麻风和HIV合并感染组、HIV感染组血浆IL-12水平均呈降低趋势,但是无统计学意义(p0.05)。结论：(1)麻风和HIV合并感染者、HIV感染者机体免疫功能减弱,与HIV感染者对比,麻风和HIV合并感染者可能未出现更严重的免疫功能异常。(2)麻风和HIV合并感染者CD4+T细胞表面CCR5高表达,CXCR4低表达,提示HIV感染可能是影响麻风和HIV合并感染者CD4+T细胞表面CCR5和CXCR4表达的主要因素。(3)麻风和HIV合并感染者、HIV感染者血浆IL-10轻度升高,IL-12水平降低,尽管与健康者对照无统计学意义,仍可初步说明两组感染者Th0细胞向Th1细胞优势分化能力减弱,存在Th1/Th2平衡向Th2偏移的趋势。(4)HIV感染者血浆IL-10与CD4+T细胞表面CCR5表达呈正相关。(5)本实验研究麻风和HIV合并感染组样本量较小,结果有待扩大样本含量进一步探讨。
[Abstract]:Objective: to explore the changes of immune status after leprosy and HIV infection in order to provide scientific basis for further prevention and control of leprosy and AIDS. Methods: the expression of chemokine receptor CCR5 and CXCR4 on CD4 and CD8 T cells was detected by flow cytometry in 3 cases of leprosy and HIV infection, 9 cases of HIV infection, 10 cases of leprosy infection and 9 cases of healthy control. The content of plasma cytokine IL-10,IL-12 was detected by double antibody sandwich ELISA and the viral load of HIV was detected by real-time fluorescence quantitative PCR (RT-PCR). Results: (1) in leprosy and HIV co-infection group, CD4 T cell absolute count and CD4/CD8 ratio were significantly decreased in HIV infection group (p0.05). Compared with leprosy and HIV co-infection group, CD4/CD8 ratio in HIV infected group was significantly lower (p0.05). After stratification of HIV infection group, there was no significant difference between leprosy and HIV co-infection group (p0.05). (2). In HIV infected group, CCR5 expression on CD4 T cell surface was slightly increased, CCR5 expression on CD8 T cell surface was slightly decreased. But there was no statistical significance (p0.05). There was no correlation between the expression of CCR5 on the surface of CD4 T cells and viral load in HIV infected group (r = 0.119 p0.05). (3), among leprosy and HIV co-infection group, leprosy infection group and HIV infection group. There was no significant difference in the expression of chemokine receptor CXCR4 on the surface of CD4 T cells in healthy control group (p0.05). (4). The expression of IL-10 in leprosy and HIV co-infection group, HIV infection group and leprosy infection group were all increased. But there was no significant difference compared with the healthy control group (p0.05). There was a positive correlation between plasma IL-10 and CD4 T cell CCR5 expression in HIV infection group (r=0.762p=0.028). (5) and HIV co-infection group. The plasma IL-12 level in HIV infected group was decreased, but there was no significant difference (p0.05). Conclusion: (1) in patients with leprosy and HIV infection, the immune function of HIV infected patients was weakened, compared with that of HIV infected patients. Leprosy and HIV co-infected patients may not have more serious immune dysfunction. (2) the expression of CCR5 on CD4 T cells was high and CXCR4 was low in leprosy and HIV co-infected patients. The results suggest that HIV infection may be the main factor affecting the expression of CCR5 and CXCR4 on CD4 T cells in patients with leprosy and HIV co-infection. (3) in patients with leprosy and HIV co-infection, the plasma IL-10 level is slightly higher and IL-12 level is lower in HIV infected patients. Although there was no significant difference between the two groups in comparison with the healthy controls, the ability of Th0 cells in the two groups to differentiate into Th1 cells was decreased. There was a tendency of Th1/Th2 balance shifting to Th2. (4) there was a positive correlation between plasma IL-10 and CCR5 expression on CD4 T cells in HIV infected patients. (5) the sample size of leprosy and HIV co-infection group was small. Results the sample size should be expanded further.
【学位授予单位】：泸州医学院
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R755;R512.91
[Abstract]:Objective: to explore the changes of immune status after leprosy and HIV infection in order to provide scientific basis for further prevention and control of leprosy and AIDS. Methods: the expression of chemokine receptor CCR5 and CXCR4 on CD4 and CD8 T cells was detected by flow cytometry in 3 cases of leprosy and HIV infection, 9 cases of HIV infection, 10 cases of leprosy infection and 9 cases of healthy control. The content of plasma cytokine IL-10,IL-12 was detected by double antibody sandwich ELISA and the viral load of HIV was detected by real-time fluorescence quantitative PCR (RT-PCR). Results: (1) in leprosy and HIV co-infection group, CD4 T cell absolute count and CD4/CD8 ratio were significantly decreased in HIV infection group (p0.05). Compared with leprosy and HIV co-infection group, CD4/CD8 ratio in HIV infected group was significantly lower (p0.05). After stratification of HIV infection group, there was no significant difference between leprosy and HIV co-infection group (p0.05). (2). In HIV infected group, CCR5 expression on CD4 T cell surface was slightly increased, CCR5 expression on CD8 T cell surface was slightly decreased. But there was no statistical significance (p0.05). There was no correlation between the expression of CCR5 on the surface of CD4 T cells and viral load in HIV infected group (r = 0.119 p0.05). (3), among leprosy and HIV co-infection group, leprosy infection group and HIV infection group. There was no significant difference in the expression of chemokine receptor CXCR4 on the surface of CD4 T cells in healthy control group (p0.05). (4). The expression of IL-10 in leprosy and HIV co-infection group, HIV infection group and leprosy infection group were all increased. But there was no significant difference compared with the healthy control group (p0.05). There was a positive correlation between plasma IL-10 and CD4 T cell CCR5 expression in HIV infection group (r=0.762p=0.028). (5) and HIV co-infection group. The plasma IL-12 level in HIV infected group was decreased, but there was no significant difference (p0.05). Conclusion: (1) in patients with leprosy and HIV infection, the immune function of HIV infected patients was weakened, compared with that of HIV infected patients. Leprosy and HIV co-infected patients may not have more serious immune dysfunction. (2) the expression of CCR5 on CD4 T cells was high and CXCR4 was low in leprosy and HIV co-infected patients. The results suggest that HIV infection may be the main factor affecting the expression of CCR5 and CXCR4 on CD4 T cells in patients with leprosy and HIV co-infection. (3) in patients with leprosy and HIV co-infection, the plasma IL-10 level is slightly higher and IL-12 level is lower in HIV infected patients. Although there was no significant difference between the two groups in comparison with the healthy controls, the ability of Th0 cells in the two groups to differentiate into Th1 cells was decreased. There was a tendency of Th1/Th2 balance shifting to Th2. (4) there was a positive correlation between plasma IL-10 and CCR5 expression on CD4 T cells in HIV infected patients. (5) the sample size of leprosy and HIV co-infection group was small. Results the sample size should be expanded further.
【学位授予单位】：泸州医学院
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R755;R512.91
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相关期刊论文 前3条
1 李拯民;艾滋病和结核病[J];临床肺科杂志;2005年02期
2 吴伟伟;段争跃;杨先旭;钟娜;;瘤型麻风合并HIV感染1例[J];中国麻风皮肤病杂志;2008年08期
3 张子宁;尚红;姜拥军;王亚男;张e，
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