Survivin和PI3K、AKT在寻常型银屑病皮损角质形成细胞中的表达及其相关性

发布时间：2018-11-27 19:50

【摘要】：目的探讨凋亡抑制蛋白Survivin和PI3K、AKT在寻常型银屑病(PV)进行期斑块状皮损角质形成细胞(KCs)中的表达水平及作用。方法临床上收集22例PV患者进行期斑块状皮损以及18例正常皮肤组织。采用免疫组织化学、Western blot和real-time quantitative PCR等方法检测22例PV患者进行期斑块状皮损和18例正常皮肤组织中KCs的Survivin、PI3K和AKT表达,并分析它们在PV皮损KCs中的相关性。使用小干扰RNA(siRNA)在HaCaT细胞中沉默AKT,并通过Western blot实验检测Survivin的表达水平。结果 PV皮损KCs中Survivin、PI3K和AKT的蛋白表达水平均高于正常皮肤组织。PV皮损KCs中Survivin和PI3K mRNA表达成正相关(r=0.4510;P=0.0351);PV皮损KCs中Survivin和AKT mRNA表达成正相关(r=0.4423;P=0.0393);在HaCaT细胞中,沉默AKT后可引起Survivin的表达下调。结论凋亡抑制蛋白Survivin与PI3K/AKT信号通路可能参与了PV的发生与发展。
[Abstract]:Objective to investigate the expression of apoptosis inhibitor protein (Survivin) and PI3K,AKT in plaque keratinocytes (KCs) of psoriasis vulgaris (PV). Methods 22 patients with PV underwent plaque lesions and 18 normal skin tissues. Immunohistochemical, Western blot and real-time quantitative PCR were used to detect the expression of KCs Survivin,PI3K and AKT in 22 patients with PV and 18 normal skin tissues, and the correlation between them in PV KCs was analyzed. AKT, was silenced by small interfering RNA (siRNA) in HaCaT cells and the expression of Survivin was detected by Western blot assay. Results the expression levels of Survivin,PI3K and AKT in KCs of PV lesions were higher than those in normal skin tissues. There was a positive correlation between Survivin and PI3K mRNA expression in KCs of PV lesions (r 0.4510 0. 351); PV) and Survivin and AKT mRNA in KCs lesions were positively correlated (r = 0.4423). In HaCaT cells, silencing of AKT could induce the down-regulation of Survivin expression. Conclusion Survivin and PI3K/AKT signaling pathway may be involved in the pathogenesis and development of PV.
【作者单位】：西安交通大学第二附属医院皮肤科;首都医科大学附属北京朝阳医院西区皮肤科;西安交通大学第一附属医院泌尿外科;
【基金】：国家自然科学基金(30960349)~~
【分类号】：R758.63

【相似文献】