TRAIL及其受体DR5、DcR1在初发和复发尖锐湿疣患者皮损中的表达及意义的研究

发布时间：2018-12-11 05:15

【摘要】：目的检测初发和复发尖锐湿疣(CA)患者皮损组织中TRAIL蛋白及其受体DR5、DcRl的表达情况,探讨TRAIL介导的细胞凋亡在尖锐湿疣发病及复发过程中可能的作用及意义。材料和方法采用S-P法,即免疫组化过氧化物酶标记链酶卵白素法,检测30例初发尖锐湿疣患者皮损组织、30例复发尖锐湿疣患者皮损组织中TRAIL蛋白及其受体DR5、DcRl的表达情况,30例正常皮肤组织作对照组。免疫组化染色结果采用半定量法判定,所得数据运用SPSS17.0统计分析软件进行统计分析。结果1、TRAIL蛋白的免疫阳性产物定位于胞膜和胞浆,多呈棕黄色颗粒状沉着,阳性细胞主要分布在棘细胞层。TRAIL蛋白在对照组、尖锐湿疣初发组、尖锐湿疣复发组的阳性表达率分别为91.67%、83.33%和80.00%,呈递减趋势；其表达强度在对照组与尖锐湿疣组间比较,对照组高于尖锐湿疣初发组和尖锐湿疣复发组,差异有统计学意义(p0.05),而在尖锐湿疣初发组和尖锐湿疣复发组,二者比较无统计学意义(p0.05)。 2、DR5蛋白的免疫阳性产物主要定位于细胞浆,多为浅黄色至棕黄色片状或弥漫性着色。染色阳性细胞主要分布在基底细胞层和棘细胞层。DR5蛋白在对照组、尖锐湿疣初发组、尖锐湿疣复发组的阳性表达率分别是53.33%、83.33%和93.33%,呈递增趋势；其表达强度在对照组与尖锐湿疣组间比较,对照组低于尖锐湿疣初发组和尖锐湿疣复发组,差异有统计学意义(P0.05)；而在尖锐湿疣初发组和尖锐湿疣复发组,二者比较无统计学意义(P0.05)。 3、DcRl蛋白的免疫阳性产物定位于胞浆和胞膜,多为浅黄色至棕黄色片状或弥漫性着色。染色阳性细胞主要分布在棘细胞层。DcR1蛋白在对照组、尖锐湿疣初发组、尖锐湿疣复发组的阳性表达率分别100%、57.50%、40.00%。呈递减趋势。其表达强度在任两组间比较差异均有统计学意义(P0.05)。结论 1、TRAIL在尖锐湿疣皮损组织中的异常低表达,可能与尖锐湿疣的发病有关。 2、TRAIL在初发组和复发组表达率无差异,提示TRAIL可能不是参与尖锐湿疣复发的主要原因。 3、死亡受体DR5在尖锐湿疣组织中表达上调,可能为外源性TRAIL应用于临床治疗提供依据。 4、诱骗受体DcRl在CA复发组表达下调,低于CA初发组,提示DcRl可能是HPV感染细胞逃避机体免疫监视,导致尖锐湿疣复发的一个原因。 5、诱骗受体DeRl在尖锐湿疣组织中表达下调,结合死亡受体DR5的表达上调,可能解释外源性TRAIL靶向性杀伤感染细胞的机制。
[Abstract]:Objective to detect the expression of TRAIL protein and its receptor DR5,DcRl in the lesions of patients with primary and recurrent condyloma acuminatum (CA) and to explore the possible role and significance of TRAIL mediated apoptosis in the pathogenesis and recurrence of condyloma acuminatum. Materials and methods S-P method was used to detect TRAIL protein and its receptor DR5, in 30 cases of primary condyloma acuminatum and 30 cases of recurrent condyloma acuminatum. The expression of DcRl in 30 normal skin tissues as control group. The results of immunohistochemical staining were determined by semi-quantitative method, and the data were analyzed by SPSS17.0 software. Results 1the immunoreactive products of trail protein were located in the cell membrane and cytoplasm. The positive cells were mainly distributed in the layer of spinous cells. TRAIL protein was found in the control group and in the primary condyloma acuminatum group. The positive expression rates of the patients with recurrent condyloma acuminatum were 91.67% and 83.33%, respectively, showing a decreasing trend. Compared with condyloma acuminatum group, the expression intensity of condyloma acuminatum in control group was higher than that in primary condyloma acuminatum group and recurrent condyloma acuminatum group (p0.05), but it was higher in primary condyloma acuminatum group and condyloma acuminatum recurrence group. There was no significant difference between the two groups (p 0.05). 2 the immunopositive products of DR5 protein were mainly located in the cytoplasm, most of them were yellowish to brownish flaky or diffuse staining. The positive expression rates of DR5 protein in control group, primary condyloma acuminatum group and recurrent condyloma acuminatum group were 53.333.33% and 93.33%, respectively. Compared with condyloma acuminatum group, the expression intensity of condyloma acuminatum in control group was lower than that in primary condyloma acuminatum group and recurrent condyloma acuminatum group (P0.05). However, there was no significant difference between the two groups in the primary condyloma acuminatum group and the recurrent condyloma acuminatum group (P0.05). 3The immunoreactive products of DcRl protein were located in the cytoplasm and membrane, and most of them were light yellow to brownish yellow flakes or diffuse staining. The positive expression rates of DcR1 protein in the control group, the primary condyloma acuminatum group and the recurrent condyloma acuminatum group were 100% and 57.50% respectively. Show a decreasing trend. There were significant differences in expression intensity between any two groups (P0.05). Conclusion 1 the abnormal low expression of trail in the lesions of condyloma acuminatum may be related to the pathogenesis of condyloma acuminatum. 2There was no difference in the expression rate of trail between the primary group and the recurrent group, suggesting that TRAIL may not be the main reason for the recurrence of condyloma acuminatum. 3. The expression of death receptor DR5 was up-regulated in condyloma acuminatum, which may provide evidence for the application of exogenous TRAIL in clinical treatment. 4. The expression of decoy receptor DcRl was down-regulated in CA recurrence group, which was lower than that in CA group, suggesting that DcRl might be a reason for HPV infection cells to evade immune surveillance and lead to recurrence of condyloma acuminatum. 5. The expression of decoy receptor DeRl was down-regulated in condyloma acuminatum, and the expression of death receptor DR5 was up-regulated, which might explain the mechanism of exogenous TRAIL targeting killing infected cells.
【学位授予单位】：山东大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R752.53

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