齐墩果酸对小鼠雄激素性脱发治疗作用的研究
发布时间：2018-12-11 18:41
【摘要】：目的：以雄激素性脱发小鼠模型为研究目标,通过在小鼠背部外用中药成分齐墩果酸观察小鼠毛发生长情况并对其血清T、E2含量、T/E2比例进行检测,探讨齐墩果酸对雄激素性脱发的治疗作用及其相关机制研究。方法：将56只B6CBAF1/J雄性小鼠按体重编号,按照随机数字表法选出13只小鼠作为空白组,余下小鼠分笼喂养。除空白组外未分组小鼠均按照5mg/Kg·d剂量给予背部皮下注射丙酸睾酮,连续给药4周,造成雄激素性脱发动物模型,空白组注射等量生理盐水。实验第4周小鼠造模成功后,在空白组及未分组小鼠中分别随机选出3只小鼠颈部脱臼处死,观察其毛发脱落情况及脱发区皮肤组织病理变化。将剩余的40只未分组小鼠分为模型组、基质组、阳性对照组、齐墩果酸组,每组10只小鼠,继续给予丙酸睾酮注射,同时齐墩果酸组外用1%齐墩果酸溶液、阳性对照组外用5%米诺地尔溶液、基质组外用未加用齐墩果酸的空白溶液,模型组不给药。第8周实验结束时,取小鼠股动脉血液,提取血清。取血后将小鼠颈部脱臼处死,平行脊柱取小鼠背部脱毛区皮肤组织做HE染色,计算终毛/毳毛比例。应用化学发光微粒子免疫分析系统及配套的人睾酮、雌二醇试剂盒测定小鼠血清内睾酮T、雌二醇E2的含量及T/E2比例,整个测定过程由仪器按预定程序自动完成。结果：1、小鼠造模2周后,背部毛发逐渐开始失去光泽,造模4周、8周的小鼠,不同程度地出现了背部毛发脱落,逐渐从头部向尾部发展,且脱落加剧；皮肤组织病理提示毛囊微型化,终毛/毳毛数目比例下降,表明雄激素性脱发小鼠模型造模成功。2、实验第8周,小鼠背部皮肤组织HE染色,计数终毛与毳毛的数目,进行终毛/毳毛比例比较,齐墩果酸组与模型组、基质组比较,比例升高(P0.01)：与空白组比较,比例下降(P0.05)；齐墩果酸组与阳性对照组之间无明显差异。3、实验第8周,比较各组小鼠之间血清T、E2含量及T/E2比例。与空白组比较,其余4组小鼠血清内睾酮T、雌二醇E2含量、T/E2比例均显著升高(P0.01),进行组间比较,各组T、E2、T/E2无明显差异(P0.05)。结论：1、B6CBAF1/J小鼠背部皮下注射丙酸睾酮建立雄激素性脱发小鼠模型的方法确实有效,造模效果及模型稳定性都较好。2、齐墩果酸治疗雄激素性脱发是有效的,其与阳性对照米诺地尔比较,促进毛发生长的疗效相当。齐墩果酸在治疗雄激素性脱发的同时还可逆转终毛与毳毛的比例、改善毛囊微型化。3、齐墩果酸外用治疗雄激素性脱发小鼠4周,不能改变小鼠体内雄激素T、雌激素E2含量、T/E2比例。
[Abstract]:Objective: to observe the hair growth of mice with androgen induced alopecia by using oleanolic acid (Oleanolic acid) on the back of mice. To investigate the therapeutic effect of oleanolic acid on androgen induced alopecia and its related mechanism. Methods: Fifty-six male B6CBAF1/J mice were selected as blank group according to their body weight number and random number table method. The remaining mice were fed in cage. All the mice except the blank group were subcutaneously injected with testosterone propionate according to the dose of 5mg/Kg d for 4 weeks to make androgen-induced alopecia animal model. The blank group was injected with the same amount of normal saline. At the 4th week of the experiment, 3 mice were randomly selected for neck dislocations and killed in blank group and undivided group. The hair loss and the pathological changes of skin tissue in the alopecia region were observed. The remaining 40 undivided mice were divided into three groups: model group, matrix group, positive control group and oleanolic acid group. 10 mice in each group were given testosterone propionate injection and oleanolic acid group was treated with 1% oleanolic acid solution. The positive control group was treated with 5% minoxidil solution, the matrix group was treated with blank solution without oleanolic acid, and the model group was not treated. At the end of the 8th week, the blood of the femoral artery of mice was taken and the serum was extracted. After blood was taken, the cervical dislocations of the mice were killed, and the skin tissues of the dorsal hair removal area of the mice were taken from the parallel spine for HE staining, and the final hair / vellus hair ratio was calculated. The contents of testosterone, estradiol E _ 2 and T/E2 ratio in serum of mice were determined by chemiluminescence microparticle immunoassay system and matched human testosterone and estradiol kit. Results: 1. After 2 weeks of modeling, the hair on the back began to lose its luster gradually. After 4 weeks and 8 weeks of modeling, the hair loss of the back appeared in varying degrees, and gradually developed from the head to the tail, and the shedding became worse. The skin histopathology indicated that the hair follicles were miniaturized and the number ratio of terminal hair to vellus hair decreased, which indicated that the model of androgen-induced alopecia was successful. 2. At the 8th week of the experiment, HE staining was used to count the number of terminal hair and vellus hair in the skin of mice. The ratio of terminal hair to vellus hair in oleanolic acid group was higher than that in model group and matrix group (P0.01); compared with blank group, the proportion of oleanolic acid group decreased (P0.05). There was no significant difference between the oleanolic acid group and the positive control group. Compared with the blank group, the levels of testosterone, estradiol E _ 2 and T/E2 in the serum of the other four groups were significantly increased (P0.01). There was no significant difference in T _ 2E _ 2 / T / E _ 2 between the four groups (P0.05). Conclusion: 1 the method of subcutaneously injecting testosterone propionate into the back of B6CBAF1 / J mice to establish androgen-induced alopecia mouse model is effective, and the model effect and model stability are good. 2, oleanolic acid is effective in the treatment of androgen-induced alopecia. Compared with the positive control minodil, the effect of promoting hair growth is comparable. Oleanolic acid can also reverse the ratio of terminal hair to vellus hair and improve the miniaturization of hair follicles. Estrogen E 2 content, T/E2 ratio.
【学位授予单位】：泸州医学院
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R758.71
本文编号：2373037
[Abstract]:Objective: to observe the hair growth of mice with androgen induced alopecia by using oleanolic acid (Oleanolic acid) on the back of mice. To investigate the therapeutic effect of oleanolic acid on androgen induced alopecia and its related mechanism. Methods: Fifty-six male B6CBAF1/J mice were selected as blank group according to their body weight number and random number table method. The remaining mice were fed in cage. All the mice except the blank group were subcutaneously injected with testosterone propionate according to the dose of 5mg/Kg d for 4 weeks to make androgen-induced alopecia animal model. The blank group was injected with the same amount of normal saline. At the 4th week of the experiment, 3 mice were randomly selected for neck dislocations and killed in blank group and undivided group. The hair loss and the pathological changes of skin tissue in the alopecia region were observed. The remaining 40 undivided mice were divided into three groups: model group, matrix group, positive control group and oleanolic acid group. 10 mice in each group were given testosterone propionate injection and oleanolic acid group was treated with 1% oleanolic acid solution. The positive control group was treated with 5% minoxidil solution, the matrix group was treated with blank solution without oleanolic acid, and the model group was not treated. At the end of the 8th week, the blood of the femoral artery of mice was taken and the serum was extracted. After blood was taken, the cervical dislocations of the mice were killed, and the skin tissues of the dorsal hair removal area of the mice were taken from the parallel spine for HE staining, and the final hair / vellus hair ratio was calculated. The contents of testosterone, estradiol E _ 2 and T/E2 ratio in serum of mice were determined by chemiluminescence microparticle immunoassay system and matched human testosterone and estradiol kit. Results: 1. After 2 weeks of modeling, the hair on the back began to lose its luster gradually. After 4 weeks and 8 weeks of modeling, the hair loss of the back appeared in varying degrees, and gradually developed from the head to the tail, and the shedding became worse. The skin histopathology indicated that the hair follicles were miniaturized and the number ratio of terminal hair to vellus hair decreased, which indicated that the model of androgen-induced alopecia was successful. 2. At the 8th week of the experiment, HE staining was used to count the number of terminal hair and vellus hair in the skin of mice. The ratio of terminal hair to vellus hair in oleanolic acid group was higher than that in model group and matrix group (P0.01); compared with blank group, the proportion of oleanolic acid group decreased (P0.05). There was no significant difference between the oleanolic acid group and the positive control group. Compared with the blank group, the levels of testosterone, estradiol E _ 2 and T/E2 in the serum of the other four groups were significantly increased (P0.01). There was no significant difference in T _ 2E _ 2 / T / E _ 2 between the four groups (P0.05). Conclusion: 1 the method of subcutaneously injecting testosterone propionate into the back of B6CBAF1 / J mice to establish androgen-induced alopecia mouse model is effective, and the model effect and model stability are good. 2, oleanolic acid is effective in the treatment of androgen-induced alopecia. Compared with the positive control minodil, the effect of promoting hair growth is comparable. Oleanolic acid can also reverse the ratio of terminal hair to vellus hair and improve the miniaturization of hair follicles. Estrogen E 2 content, T/E2 ratio.
【学位授予单位】：泸州医学院
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R758.71
【参考文献】
相关期刊论文 前9条
1 胡华杰;;齐墩果酸药理作用与临床应用研究进展[J];海峡药学;2012年03期
2 范卫新,朱文元;55种中药对小鼠触须毛囊体外培养生物学特性的研究[J];临床皮肤科杂志;2001年02期
3 杨蕊;苏乐群;黄欣;;齐墩果酸自微乳的制备及质量评价[J];食品与药品;2008年01期
4 张兴洪,刘彦群,魏志平,田美华,马海军;中药煎剂对体外培养的猪毛囊毛发生长及毛囊内VEGF表达的影响[J];徐州医学院学报;2004年06期
5 任骏,王振纲;齐墩果酸对前列腺素及环核苷酸的影响[J];中国药理学通报;1991年03期
6 岂红娇;王友信;赵俊英;王嵬;;雄激素源性脱发患者雄激素受体基因多态性分析[J];中国皮肤性病学杂志;2011年03期
7 范卫新,朱文元;女贞子等中药对小鼠毛囊生长周期中生长因子mRNA表达的影响[J];中华皮肤科杂志;2000年04期
8 张东方,黄炜,黄济群,张瑞玲,廖兆全;齐墩果酸抗人肺癌细胞增殖、侵袭和诱导细胞凋亡的研究[J];肿瘤防治研究;2003年03期
9 吴勃岩;高明;徐绍娜;;女贞子有效成分齐墩果酸对S180荷瘤小鼠抑瘤作用及存活时间的影响[J];中医药信息;2010年01期
，本文编号：2373037
本文链接：https://www.wllwen.com/yixuelunwen/pifb/2373037.html
最近更新
教材专著
