基于新型光学成像技术的小鼠皮肤光老化及修复机制的实验研究
发布时间：2018-12-17 21:47
【摘要】：本文利用多光子显微成像技术的先进手段,以亚微米尺度观察在紫外灯辐照下的老化皮肤在不同光源的作用下皮肤胶原重构的情况,揭示光不同光源与生物组织相互作用产生的效果,进一步地理解老化皮肤在激光作用下的机制。同时利用OCT的高分辨率和非侵入式测量的特点,对不同光源的嫩肤效果进行初步比较,得到光老化皮肤在嫩肤过程中的结构改变和散射系数的变化情况。 首先,介绍了光美容技术发展的历史和现状,论述了光美容治疗过程中的疗效评价手段以及OCT技术、多光子显微技术在光美容诊断上的应用及发展,阐述了本课题的研究意义。同时,对皮肤的结构进行简要的介绍,讨论了光子嫩肤的作用机理、剂量选择的基本原理。并结合本文实际使用的仪器,探讨仪器应用的可行性和使用方式。 其次,利用多光子显微成像技术,从亚微米数量级观察不同光源对皮肤的作用效果,分析真皮层胶原纤维的变化情况,并结合HE染色切片进行观察。 最后,采用了OCT系统比较非消融性的IPL和消融性的Er：YAG激光的作用效果。观察不同光源及不同能量密度作用下的生物组织体现的散射光强以及散射系数之间存在着明显变化和区别。
[Abstract]:In this paper, we use the advanced technique of multiphoton microscopic imaging to observe the collagen remodeling of aging skin irradiated by ultraviolet lamp under different light sources at sub-micron scale. The effects of interaction between different light sources and biological tissues were revealed, and the mechanism of aging skin under laser irradiation was further understood. At the same time, the characteristics of high resolution and non-invasive measurement of OCT are used to compare the skin effect of different light sources, and the changes of structure and scattering coefficient of photoaged skin in the process of skin rejuvenation are obtained. Firstly, this paper introduces the history and present situation of the development of light beauty technology, discusses the evaluation method of the curative effect in the process of light beauty therapy, the application and development of OCT technology and multiphoton microscopy technology in the diagnosis of light beauty, and expounds the research significance of this subject. At the same time, the structure of skin is briefly introduced, and the mechanism of photon tender skin and the basic principle of dose selection are discussed. Combined with the instrument used in this paper, the feasibility and application of the instrument are discussed. Secondly, the effects of different light sources on the skin were observed by using multi-photon microscopic imaging technique, and the changes of collagen fibers in the dermis were analyzed, and the changes of collagen fibers in the dermis were observed by HE staining. Finally, OCT system is used to compare the effect of nonablative IPL and ablative Er:YAG laser. The variation and difference of scattering intensity and scattering coefficient of biological tissue under different light sources and different energy density were observed.
【学位授予单位】：福建师范大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R758.1
本文编号：2384839
[Abstract]:In this paper, we use the advanced technique of multiphoton microscopic imaging to observe the collagen remodeling of aging skin irradiated by ultraviolet lamp under different light sources at sub-micron scale. The effects of interaction between different light sources and biological tissues were revealed, and the mechanism of aging skin under laser irradiation was further understood. At the same time, the characteristics of high resolution and non-invasive measurement of OCT are used to compare the skin effect of different light sources, and the changes of structure and scattering coefficient of photoaged skin in the process of skin rejuvenation are obtained. Firstly, this paper introduces the history and present situation of the development of light beauty technology, discusses the evaluation method of the curative effect in the process of light beauty therapy, the application and development of OCT technology and multiphoton microscopy technology in the diagnosis of light beauty, and expounds the research significance of this subject. At the same time, the structure of skin is briefly introduced, and the mechanism of photon tender skin and the basic principle of dose selection are discussed. Combined with the instrument used in this paper, the feasibility and application of the instrument are discussed. Secondly, the effects of different light sources on the skin were observed by using multi-photon microscopic imaging technique, and the changes of collagen fibers in the dermis were analyzed, and the changes of collagen fibers in the dermis were observed by HE staining. Finally, OCT system is used to compare the effect of nonablative IPL and ablative Er:YAG laser. The variation and difference of scattering intensity and scattering coefficient of biological tissue under different light sources and different energy density were observed.
【学位授予单位】：福建师范大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R758.1
【引证文献】
相关硕士学位论文 前2条
1 徐小晖;小鼠皮肤深Ⅱ度烫伤及治疗的光学观察与分析[D];福建师范大学;2012年
2 陈新光;检测神经纤维膜电位动力学的光学二次谐波编码及其数值模拟[D];福建师范大学;2012年
，本文编号：2384839
本文链接：https://www.wllwen.com/yixuelunwen/pifb/2384839.html
最近更新
教材专著
