人类疱疹病毒8型与非卡波氏肉瘤组织相关性的初步研究
发布时间：2018-12-26 07:22
【摘要】： 目的:检测人类疱疹病毒8型(HHV-8)在四种非Kaposi肉瘤皮损组织中的感染情况,以初步探讨HHV-8与皮肤鳞状细胞癌、蕈样肉芽肿、Castleman病和化脓性肉芽肿相关性研究。方法:使用酚-氯仿-异戊醇方法对新疆维吾尔自治区人民医院皮肤性病科、病理科从2004年到2009年收集的30例鳞状细胞癌、20例Castleman病(其中5例为多中性Castleman病)、13例蕈样肉芽肿、20例化脓性肉芽肿石蜡包埋组织进行HHV-8 DNA抽提;然后使用巢式PCR方法对抽提的DNA进行ORF26基因片段的扩增,扩增产物在1.5%的琼脂糖凝胶上电泳,并在凝胶成像系统下观察电泳结果,从而确定ORF26基因阳性标本;然后计算HHV-8在各种标本中的阳性率。结果:30例皮肤鳞状细胞癌皮损组织有5份为阳性(16.7%)。13例蕈样肉芽肿石蜡组织有1例为阳性(7.7%),20例化脓性肉芽肿和20例Castleman病石蜡组织中均未检出HHV-8。结论:①HHV-8可能是新疆皮肤鳞状细胞癌发病原因之一。②HHV-8与蕈样肉芽肿的发病可能不具有相关性,HHV-8可能是MF发生后偶发的一种附带感染。③Castleman病和化脓性肉芽肿的发病可能均与HHV-8无关。
[Abstract]:Objective: to detect the infection of human herpesvirus 8 (HHV-8) in four kinds of non Kaposi sarcomas and to explore the relationship between HHV-8 and cutaneous squamous cell carcinoma, mycosis fungoides, Castleman disease and suppurative granuloma. Methods: using phenol-chloroform-isoamyl alcohol method, 30 cases of squamous cell carcinoma (SCC) and 20 cases of Castleman disease (5 cases of polyneutral Castleman disease) were collected from 2004 to 2009 in the Department of Dermatology and venereal Diseases of the people's Hospital of Xinjiang Uygur Autonomous region. 13 cases of fungoid granuloma and 20 cases of suppurative granuloma paraffin embedded tissue were extracted by HHV-8 DNA. Then nested PCR method was used to amplify the ORF26 gene fragment of extracted DNA. The amplified product was electrophoresis on 1.5% agarose gel. The results of electrophoresis were observed under the gel imaging system to determine the positive samples of ORF26 gene. Then the positive rate of HHV-8 in various specimens was calculated. Results: 5 cases (16.7%) were positive in skin lesions of 30 cases of cutaneous squamous cell carcinoma and 1 case (7.7%) of paraffin tissues of 13 cases of mycosis fungoides. HHV-8. was not detected in 20 cases of suppurative granuloma and 20 cases of Castleman's disease in paraffin tissues. Conclusion: 1HHV-8 may be one of the causes of cutaneous squamous cell carcinoma in Xinjiang. 2HHV-8 may not be associated with the pathogenesis of mycosis fungoides. HHV-8 may be an incidental infection after MF. 3Castleman disease and purulent granuloma may not be associated with HHV-8.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R739.5
[Abstract]:Objective: to detect the infection of human herpesvirus 8 (HHV-8) in four kinds of non Kaposi sarcomas and to explore the relationship between HHV-8 and cutaneous squamous cell carcinoma, mycosis fungoides, Castleman disease and suppurative granuloma. Methods: using phenol-chloroform-isoamyl alcohol method, 30 cases of squamous cell carcinoma (SCC) and 20 cases of Castleman disease (5 cases of polyneutral Castleman disease) were collected from 2004 to 2009 in the Department of Dermatology and venereal Diseases of the people's Hospital of Xinjiang Uygur Autonomous region. 13 cases of fungoid granuloma and 20 cases of suppurative granuloma paraffin embedded tissue were extracted by HHV-8 DNA. Then nested PCR method was used to amplify the ORF26 gene fragment of extracted DNA. The amplified product was electrophoresis on 1.5% agarose gel. The results of electrophoresis were observed under the gel imaging system to determine the positive samples of ORF26 gene. Then the positive rate of HHV-8 in various specimens was calculated. Results: 5 cases (16.7%) were positive in skin lesions of 30 cases of cutaneous squamous cell carcinoma and 1 case (7.7%) of paraffin tissues of 13 cases of mycosis fungoides. HHV-8. was not detected in 20 cases of suppurative granuloma and 20 cases of Castleman's disease in paraffin tissues. Conclusion: 1HHV-8 may be one of the causes of cutaneous squamous cell carcinoma in Xinjiang. 2HHV-8 may not be associated with the pathogenesis of mycosis fungoides. HHV-8 may be an incidental infection after MF. 3Castleman disease and purulent granuloma may not be associated with HHV-8.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R739.5
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