cFLIP在人皮肤鳞状细胞癌中的表达和意义
发布时间：2019-01-05 06:36
【摘要】：目的:观察皮肤鳞状细胞癌(SCC)和正常皮肤组织和细胞中抗凋亡蛋白cFLIP的表达情况,探讨其与SCC临床病理特征的关系及意义。 方法:采用免疫组织化学亲和素-生物素-过氧化物酶(Avidin-Biotin-Peroxidase Complex,SABC)法检测52例皮肤鳞状细胞癌皮损和13例正常对照皮肤组织中cFLIP表达;同时用逆转录PCR(RT-PCR)和免疫印迹(western-blot)方法检测人皮肤鳞癌细胞系A431和永生化角质形成细胞系Hacat中cFLIP各自的表达情况。 结果:皮肤鳞状细胞癌组织中cFLIP表达的阳性率为67.31%,而正常皮肤组织中的阳性率为15.38%,两者表达有显著性差异(P0.01);cFLIP表达与病理分级有无淋巴结转移相关,与性别年龄病程发病部位无关;cFLIP和细胞性增殖核抗原(PCNA)在皮肤鳞状细胞癌中的表达具有相关性(r=0.781,P0.01);人皮肤鳞癌细胞系A431中cFLIP的mRNA和蛋白表达水平均高于永生化角质形成细胞系Hacat。 结论:cFLIP在皮肤鳞状细胞癌中表达显著高于正常皮肤,cFLIP与肿瘤的病理学分级有无淋巴结转移相关,且和PCNA具有相关性;可能在皮肤鳞状细胞癌的发生发展中发挥重要作用。。
[Abstract]:Objective: to investigate the expression of antiapoptotic protein (cFLIP) in (SCC) and normal skin tissues and cells of squamous cell carcinoma of skin (SCC), and to explore the relationship between the expression and clinicopathological features of SCC. Methods: immunohistochemical avidin-biotin-peroxidase (Avidin-Biotin-Peroxidase Complex,SABC) method was used to detect the expression of cFLIP in 52 cases of cutaneous squamous cell carcinoma and 13 cases of normal control. The expression of cFLIP in human cutaneous squamous cell carcinoma cell line A431 and immortalized keratinocyte cell line Hacat was detected by reverse transcription PCR (RT-PCR) and Western blot (western-blot). Results: the positive rate of cFLIP expression was 67.31 in squamous cell carcinoma of skin and 15.38 in normal skin tissue. There was significant difference between them (P0.01); cFLIP expression and pathological grade). Is there a lymph node metastasis associated with sex? Age? Course? The expression of cFLIP and cellular proliferating nuclear antigen (PCNA) in squamous cell carcinoma of skin was correlated (r = 0.781P 0.01). The expression of cFLIP mRNA and protein in human cutaneous squamous cell carcinoma cell line A431 was higher than that in immortalized keratinocyte cell line Hacat.. Conclusion: the expression of cFLIP in cutaneous squamous cell carcinoma is significantly higher than that in normal skin. Lymph node metastasis is associated with PCNA, which may play an important role in the development of cutaneous squamous cell carcinoma.
【学位授予单位】：华中科技大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R739.5
本文编号：2401417
[Abstract]:Objective: to investigate the expression of antiapoptotic protein (cFLIP) in (SCC) and normal skin tissues and cells of squamous cell carcinoma of skin (SCC), and to explore the relationship between the expression and clinicopathological features of SCC. Methods: immunohistochemical avidin-biotin-peroxidase (Avidin-Biotin-Peroxidase Complex,SABC) method was used to detect the expression of cFLIP in 52 cases of cutaneous squamous cell carcinoma and 13 cases of normal control. The expression of cFLIP in human cutaneous squamous cell carcinoma cell line A431 and immortalized keratinocyte cell line Hacat was detected by reverse transcription PCR (RT-PCR) and Western blot (western-blot). Results: the positive rate of cFLIP expression was 67.31 in squamous cell carcinoma of skin and 15.38 in normal skin tissue. There was significant difference between them (P0.01); cFLIP expression and pathological grade). Is there a lymph node metastasis associated with sex? Age? Course? The expression of cFLIP and cellular proliferating nuclear antigen (PCNA) in squamous cell carcinoma of skin was correlated (r = 0.781P 0.01). The expression of cFLIP mRNA and protein in human cutaneous squamous cell carcinoma cell line A431 was higher than that in immortalized keratinocyte cell line Hacat.. Conclusion: the expression of cFLIP in cutaneous squamous cell carcinoma is significantly higher than that in normal skin. Lymph node metastasis is associated with PCNA, which may play an important role in the development of cutaneous squamous cell carcinoma.
【学位授予单位】：华中科技大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R739.5
【参考文献】
相关期刊论文 前2条
1 高思海,潘铁成,赵金平,李军;食管鳞癌FLIP表达与细胞凋亡相关[J];世界华人消化杂志;2004年06期
2 陶娟;董婧;李延;刘业强;杨井;涂亚庭;;凋亡抑制蛋白c-FLIP在带状疱疹患者细胞免疫中作用的研究[J];中国皮肤性病学杂志;2008年04期
，本文编号：2401417
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