内源性omega-3多元不饱和脂肪酸对小鼠银屑病样皮损以及Th17细胞的影响

发布时间：2019-01-05 20:28

【摘要】：目的观察内源性omega-3多元不饱和脂肪酸(polyunsaturated fatty acids,PUFAs)对小鼠银屑病样皮损以及脾脏Th17细胞因子的影响。方法本实验采用Fat-1转基因小鼠模型,将野生型(Wild-type)小鼠和Fat-1小鼠各分为2组,背部以及右耳背外涂咪喹莫特乳膏(Imiquimod,IMQ)和凡士林乳膏(cream),建立银屑病样皮损模型及对照组。观察4组小鼠给药部位皮肤变化,对表皮组织进行病理切片和HE染色,流式细胞术测定脾细胞内Th17细胞因子的百分比。结果 Fat-1 IMQ与WT IMQ组相比,表皮因IMQ作用而出现角质层、棘细胞层的增厚,大体表现的红斑、增厚、鳞屑反应均较后者轻微,同时IMQ所造成的脾细胞内Th17细胞以及炎症因子IL-17升高,也在Fat-1组小鼠中呈现出了较低的水平(P0.05)。而两组cream组间差异无统计学意义(P0.05)。结论内源性omega-3 PUFAs在银屑病中能够减少脾细胞内Th17细胞的水平,减少IL-17炎症因子的分泌以及改善银屑病样红斑、鳞屑、增厚等临床症状,从而发挥治疗银屑病的作用。
[Abstract]:Objective to observe the effects of endogenous omega-3 polyunsaturated fatty acid (polyunsaturated fatty acids,PUFAs) on psoriatic lesions and Th17 cytokines in spleen of mice. Methods Fat-1 transgenic mice were used in this experiment. Wild type (Wild-type) mice and Fat-1 mice were divided into two groups: the back and the right ear were coated with Imiquimod Cream (Imiquimod,IMQ) and Vaseline Cream (cream), respectively. To establish psoriasis-like skin lesion model and control group. The skin changes of the four groups were observed, the epidermal tissues were stained with HE and pathological sections, and the percentage of Th17 cytokines in spleen cells was determined by flow cytometry. Results compared with the WT IMQ group, the keratinocytes appeared in the epidermis due to the action of IMQ, the thickening of the spinous cell layer, the erythema, thickening and the scale reaction of the Fat-1 IMQ group were more slight than those of the latter group. At the same time, the increase of Th17 cells and inflammatory factor IL-17 in spleen cells caused by IMQ also showed a lower level in Fat-1 group (P0.05). There was no significant difference in cream between the two groups (P0.05). Conclusion Endogenous omega-3 PUFAs can reduce the level of Th17 cells in splenic cells, decrease the secretion of IL-17 inflammatory factors and improve the clinical symptoms of psoriasis like erythema, scales and thickening in psoriasis, so as to play a role in the treatment of psoriasis.
【作者单位】：南方医科大学附属南粤医院广东省第二人民医院皮肤性病科;南方医科大学第三临床医学院;
【分类号】：R758.63

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