甲真菌病患者分离真菌多样性分析

发布时间：2019-01-05 20:54

【摘要】：为了探寻甲真菌病患者病甲部位的真菌多样性,以期对临床治疗提供参考,结合菌种培养的形态学研究和rDNA ITS序列分析的方法,对临床拟诊为甲真菌病患者的病甲分离培养菌株进行物种鉴定,并以丰富性指数(Ma)、多样性指数(H′)、均匀度指数(J)等指标分析指甲和趾甲分离真菌的多样性。2010年2月至9月在北京大学第三医院皮肤科的1 568例拟诊甲真菌病患者中,641例培养阳性,包括趾甲561例和指甲80例。检测到的皮肤癣菌占59.13%(其中红色毛癣菌为96.04%),酵母菌17.00%,其他真菌23.87%。分离自趾甲的Candida galli、Lewia infectoria和Paraconiothyrium hawaiiense,以及分离自指甲的Cyberlindnera jadinii确定为中国新记录种。趾甲分离菌的物种多样性指数H′、丰富度指数Ma和均匀度指数J均高于指甲分离菌。研究结果表明,该医院就诊甲真菌病患者可分离培养的真菌以皮肤癣菌的红色毛癣菌为主要物种,且趾甲分离菌的菌种多样性较指甲的更为明显。
[Abstract]:In order to explore the diversity of fungi in nail parts of patients with onychomycosis, and to provide reference for clinical treatment, the methods of morphological study and rDNA ITS sequence analysis were used to study the culture of onychomycosis. The isolated and cultured strains of onychomycosis were identified, and the richness index (Ma), diversity index (H') was used. Evenness index (J) was used to analyze the diversity of fungi isolated from fingernails and toenails. From February to September 2010, 641 of 1 568 patients with onychomycosis in Department of Dermatology, Peking University third Hospital were cultured positive. There were 561 toenails and 80 fingernails. The percentage of dermatophytes detected was 59.13% (96.04% for Trichophyton rubrum), 17.00% for yeast and 23.87% for other fungi. Candida galli,Lewia infectoria and Paraconiothyrium hawaiiense, isolated from toenail and Cyberlindnera jadinii isolated from fingernail were identified as new records in China. The species diversity index (H), richness index (Ma) and evenness index (J) of toenail isolates were higher than those of fingernail isolates. The results showed that the main species of fungi isolated from patients with onychomycosis were Trichophyton rubrum of dermatophytes, and the diversity of toenail isolates was more obvious than that of fingernails.
【作者单位】：中国科学院微生物研究所真菌学国家重点实验室;北京大学第三医院皮肤科真菌实验室;
【基金】：教育部创新团队(No.IRT1003)
【分类号】：R756

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