莪术提取物对非黑色素性皮肤癌小鼠TIMP-2、TIMP-1、nm23及p53表达的影响

发布时间：2019-01-22 20:40

【摘要】：目的探讨莪术提取物对非黑色素性皮肤癌小鼠TIMP-2、TIMP-1、nm23及p53表达的影响。方法将45只雄性KM小鼠随机分为模型组、对照组及莪术组,每组15只。模型组和莪术组小鼠皮肤上涂含60%二甲基苯蒽的矿物油建立非黑色素性皮肤癌模型,对照组小鼠皮肤上涂不含二甲苯崽的矿物油,位置及剂量同模型组,1次/d,连续10 d,从造模后第2天开始,莪术组在造模处皮肤上涂抹莪术水提取物乳液,约0.8 g。模型组和对照组均以等体积的生理盐水涂抹,1次/d,连续4周。结果 (1)与模型组相比,莪术组TIMP-1、TIMP-2、nm23、p53阳性率及表达强度明显升高,差异有统计学意义(均P0.05);(2)病理切片可见模型组恶性程度高,毛细血管增生明显,细胞核固缩,细胞异型性明显,局部可见坏死,肿瘤细胞发生转移。莪术组可见肿瘤周边的浸润较局限,部分细胞异型性明显,与模型组相比,差异显著。结论莪术提取物对非黑色素性皮肤癌小鼠TIMP-2、TIMP-1、nm23与p53表达有改善作用,对临床有指导意义。
[Abstract]:Objective to investigate the effect of curcuma curcumae extract on the expression of TIMP-2,TIMP-1,nm23 and p53 in mice with non melanoma skin cancer. Methods 45 male KM mice were randomly divided into three groups: model group, control group and curcuma group with 15 mice in each group. The model group and zedoary group mice were coated with 60% dimethyl benzene anthracene mineral oil on the skin to establish the model of non-melanin skin cancer. The control group mice skin was coated with mineral oil without xylene cub, the position and dosage were the same as the model group once a day. For 10 consecutive days, from the second day after modeling, the curcuma group was smeared with water extract emulsion of Curcuma curcumae on the skin of the model, about 0.8 g. The model group and control group were smeared with the same volume of saline once a day for 4 weeks. Results (1) compared with the model group, the positive rate and the expression intensity of TIMP-1,TIMP-2,nm23,p53 in the zedoary group were significantly higher than those in the model group (P0.05). (2) the pathological section showed that the model group had a high degree of malignancy, obvious capillary hyperplasia, nuclear pyknosis, obvious cellular dysplasia, local necrosis and metastasis of tumor cells. The infiltration around the tumor was limited in the zedoary group, and the heterogeneity of some cells was obvious, compared with the model group, the difference was significant. Conclusion the Rhizoma Curcumae extract can improve the expression of TIMP-2,TIMP-1,nm23 and p53 in mice with non melanoma skin cancer.
【作者单位】：河北省唐山市工人医院皮肤科;
【分类号】：R739.5

【参考文献】