小鼠皮肤移植细胞有效重建毛发

发布时间：2019-02-17 13:03

【摘要】：目的研究将新鲜分离的新生小鼠皮肤细胞移植到免疫缺陷鼠上有效重建毛囊的新技术。方法新生C57小鼠取其皮肤,将表皮和真皮细胞分别分离并混合,做成高浓度的细胞悬液,移植到免疫缺陷鼠NU/NU,CB-17 SCID,NOD SCID上,观察皮肤再生情况。结果细胞移植2周之后,伤口处肉眼可见黑色皮肤形成,3周后长出大量毛发,8周后观察重建的毛发维持良好状态。没有移植细胞的对照观察到伤口愈合但没有毛发形成。组织学分析显示再生的皮肤含有成熟的毛囊和毛干并连有皮脂腺及真皮毛乳头,并且新形成的皮肤不仅有表皮层和真皮层,还有皮下脂肪层,表明移植的细胞可重建完整的皮肤。细胞移植到三种不同的免疫小鼠NU/NU,CB-17 SCID和NOD SCID上,毛囊重建的结果相似。结论创建了一套分离和移植皮肤细胞的技术,可在免疫缺陷鼠上高效地重建毛发。该技术可以用于毛囊再生的研究,并具有潜在的临床应用价值。
[Abstract]:Objective to study a new technique for effectively reconstructing hair follicles by transplanting freshly isolated skin cells from newborn mice to immunodeficient mice. Methods the skin of newborn C57 mice was harvested, the epidermis and dermis cells were separated and mixed separately, and then high concentration of cell suspension was made. The skin regeneration was observed on NU/NU,CB-17 SCID,NOD SCID of immunodeficient mice. Results after 2 weeks of cell transplantation, black skin could be seen in the naked eye of the wound, and a large number of hair was grown after 3 weeks. After 8 weeks, the reconstructed hair was observed to be in good condition. No graft cells were found to heal but no hair formation was observed. Histological analysis showed that the regenerated skin contained mature hair follicles and dried hair with sebaceous glands and true fur nipples, and the newly formed skin had not only epidermis and dermis, but also a subcutaneous fat layer. This suggests that transplanted cells can reconstruct intact skin. The results of hair follicle reconstruction were similar when the cells were transplanted to NU/NU,CB-17 SCID and NOD SCID in three different immunized mice. Conclusion A set of techniques to isolate and transplant skin cells can be used to reconstruct hair efficiently in immunodeficient mice. This technique can be used in the study of hair follicle regeneration and has potential clinical application value.
【作者单位】：济南市妇幼保健院妇产科;济南磐升生物技术有限公司;山东大学口腔医学院;
【基金】：山东省泰山学者海外特聘教授基金
【分类号】：R758.71

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