皮肤鳞状细胞癌组织Syndecan-1和乙酰肝素酶表达及临床意义
发布时间：2019-04-10 19:16
【摘要】：目的近期研究显示,Syndecan-1和乙酰肝素酶(heparanase,HPA-1)可能参与多种恶性肿瘤的侵袭和转移,但关于两者与皮肤鳞状细胞癌(cutaneous squamous cell carcinoma,CSCC)的关系较少报道。本研究旨在探讨Syndecan-1和HPA-1在CSCC组织中的表达及其与临床病理特征的关系。方法应用免疫组化方法、蛋白质印迹法和实时荧光定量PCR技术检测2009-09-01-2014-03-31潍坊市人民医院皮肤科93例CSCC及30名正常表皮中Syndecan-1和HPA-1蛋白及mRNA的表达水平。结果正常表皮、原位鳞癌、高分化鳞癌、中分化鳞癌和低分化鳞癌中Syndecan-1强阳性率分别为70.00%(21/30)、52.28%(11/21)、37.04%(10/27)、23.33%(7/30)和6.67%(1/15),CSCC Syndecan-1表达强度显著低于正常表皮对照组,χ2=13.17,P0.01;在不同肿瘤厚度和分化程度的鳞癌中,SDC1表达强度随肿瘤厚度的增加(χ2=11.66,P0.01)和分化程度的降低(χ2=12.51,P0.01)而有下降趋势;CSCC患者中伴淋巴结转移组Syndecan-1表达强度显著低于无淋巴结转移组,χ2=5.78,P0.05。CSCC组织Syndecan-1mRNA和蛋白表达水平显著低于正常对照组,且随着组织学分级的降低而依次降低(rs值分别为0.829和0.644,均P0.001)。正常表皮、原位鳞癌、高分化鳞癌、中分化鳞癌和低分化鳞癌中HPA-1阳性率分别为3.33%(1/30)、38.10%(8/21)、62.96%(15/27)、66.67%(20/30)和86.67%(13/15),CSCC中HPA-1表达强度显著高于对照组,χ2=29.52,P0.01。在不同肿瘤厚度和分化程度的鳞癌中,HPA-1表达强度随肿瘤厚度的增加(χ2=4.70,P0.05)和分化程度的降低(χ2=9.15,P0.05)而有增加的趋势,CSCC患者中伴淋巴转移组HPA-1表达强度(80.95%)显著高于无淋巴结转移组(54.17%),χ2=4.87,P0.05。CSCC组织HPA-1mRNA和蛋白表达水平显著高于正常对照组,且随着组织学分级的降低而依次升高(rs值分别为-0.793和-0.718,均P0.001)。CSCC中Syndecan-1与HPA-1表达呈负相关,rs=-0.473,P0.01。结论 Syndecan-1表达降低与HPA-1表达增强可能促进CSCC的侵袭和转移。
[Abstract]:Aim recent studies have shown that Syndecan-1 and heparanase (heparanase,HPA-1) may be involved in the invasion and metastasis of various malignant tumors, but there are few reports about their relationship with (cutaneous squamous cell carcinoma,CSCC in cutaneous squamous cell carcinoma (CSCC). The purpose of this study was to investigate the expression of Syndecan-1 and HPA-1 in CSCC and its relationship with clinicopathological features. Methods Immunohistochemistry was used. Protein blot and real-time fluorescence quantitative PCR were used to detect the expression of Syndecan-1, HPA-1 and mRNA in 93 cases of CSCC and 30 normal epidermis of Weifang people's Hospital. Results the strong positive rates of Syndecan-1 in normal epidermis, squamous cell carcinoma in situ, well differentiated squamous cell carcinoma, moderately differentiated squamous cell carcinoma and poorly differentiated squamous cell carcinoma were 70.00% (21 / 30), 52.28% (11 / 21), 37.04% (10 / 27), respectively. The expression intensity of 23. 33% (7? 30) and 6. 67% (1? 15), CSCC Syndecan-1) was significantly lower than that of the normal epidermis control group (蠂 2, 13. 17, P 0. 01). In squamous cell carcinoma with different tumor thickness and differentiation degree, the expression intensity of SDC1 decreased with the increase of tumor thickness (蠂 ~ 2 / 11.66, P0.01) and the degree of differentiation (蠂 ~ 2 / 12.51, P 0.01). The expression of Syndecan-1 in CSCC patients with lymph node metastasis was significantly lower than that in patients without lymph node metastasis, and the expression level of Syndecan-1mRNA and protein in CSCC tissues was significantly lower than that in normal controls (蠂 2 = 5.78, P0.05.CSCC, P < 0.05). And with the decrease of histologic grade (RS = 0.829 and 0.644, all P 0.001), the histological grade decreased with the decrease of the grade of histology (RS = 0.829 and 0.644, P 0.001). The positive rates of HPA-1 in normal epidermis, squamous cell carcinoma in situ, well differentiated squamous cell carcinoma, moderately differentiated squamous cell carcinoma and poorly differentiated squamous cell carcinoma were 3.33% (1 / 30), 38.10% (8 / 21), 62.96% (15 / 27), respectively. The expression intensity of HPA-1 in 66.67% (20 / 30) and 86.67% (13 / 15), CSCC) was significantly higher than that in the control group (蠂 2, 29.52, P 0.01). In squamous cell carcinoma with different tumor thickness and differentiation degree, the expression intensity of HPA-1 increased with the increase of tumor thickness (蠂 ~ 2 / 4.70, P 0.05) and the degree of differentiation (蠂 ~ 2 / 9.15, P 0.05), and the expression intensity increased with the increase of tumor thickness (蠂 ~ 2 / 4.70, P < 0.05). The expression of HPA-1 in CSCC patients with lymph node metastasis (80.95%) was significantly higher than that in patients without lymph node metastasis (54.17%). The expression of HPA-1mRNA and protein in P0.05.CSCC tissue was significantly higher than that in normal controls (蠂 2 = 4.87, P 0.05.CSCC). The expression of Syndecan-1 was negatively correlated with the expression of HPA-1, and the expression of rs=-0.473,P0.01. was negatively correlated with the decrease of histological grade (s =-0.793 and-0.718, P 0.001). Conclusion the decreased expression of Syndecan-1 and increased expression of HPA-1 may promote the invasion and metastasis of CSCC.
【作者单位】： 潍坊市人民医院皮肤科;潍坊市人民医院病理科;
【基金】：潍坊市科技发展计划(201302005)
【分类号】：R739.5
[Abstract]:Aim recent studies have shown that Syndecan-1 and heparanase (heparanase,HPA-1) may be involved in the invasion and metastasis of various malignant tumors, but there are few reports about their relationship with (cutaneous squamous cell carcinoma,CSCC in cutaneous squamous cell carcinoma (CSCC). The purpose of this study was to investigate the expression of Syndecan-1 and HPA-1 in CSCC and its relationship with clinicopathological features. Methods Immunohistochemistry was used. Protein blot and real-time fluorescence quantitative PCR were used to detect the expression of Syndecan-1, HPA-1 and mRNA in 93 cases of CSCC and 30 normal epidermis of Weifang people's Hospital. Results the strong positive rates of Syndecan-1 in normal epidermis, squamous cell carcinoma in situ, well differentiated squamous cell carcinoma, moderately differentiated squamous cell carcinoma and poorly differentiated squamous cell carcinoma were 70.00% (21 / 30), 52.28% (11 / 21), 37.04% (10 / 27), respectively. The expression intensity of 23. 33% (7? 30) and 6. 67% (1? 15), CSCC Syndecan-1) was significantly lower than that of the normal epidermis control group (蠂 2, 13. 17, P 0. 01). In squamous cell carcinoma with different tumor thickness and differentiation degree, the expression intensity of SDC1 decreased with the increase of tumor thickness (蠂 ~ 2 / 11.66, P0.01) and the degree of differentiation (蠂 ~ 2 / 12.51, P 0.01). The expression of Syndecan-1 in CSCC patients with lymph node metastasis was significantly lower than that in patients without lymph node metastasis, and the expression level of Syndecan-1mRNA and protein in CSCC tissues was significantly lower than that in normal controls (蠂 2 = 5.78, P0.05.CSCC, P < 0.05). And with the decrease of histologic grade (RS = 0.829 and 0.644, all P 0.001), the histological grade decreased with the decrease of the grade of histology (RS = 0.829 and 0.644, P 0.001). The positive rates of HPA-1 in normal epidermis, squamous cell carcinoma in situ, well differentiated squamous cell carcinoma, moderately differentiated squamous cell carcinoma and poorly differentiated squamous cell carcinoma were 3.33% (1 / 30), 38.10% (8 / 21), 62.96% (15 / 27), respectively. The expression intensity of HPA-1 in 66.67% (20 / 30) and 86.67% (13 / 15), CSCC) was significantly higher than that in the control group (蠂 2, 29.52, P 0.01). In squamous cell carcinoma with different tumor thickness and differentiation degree, the expression intensity of HPA-1 increased with the increase of tumor thickness (蠂 ~ 2 / 4.70, P 0.05) and the degree of differentiation (蠂 ~ 2 / 9.15, P 0.05), and the expression intensity increased with the increase of tumor thickness (蠂 ~ 2 / 4.70, P < 0.05). The expression of HPA-1 in CSCC patients with lymph node metastasis (80.95%) was significantly higher than that in patients without lymph node metastasis (54.17%). The expression of HPA-1mRNA and protein in P0.05.CSCC tissue was significantly higher than that in normal controls (蠂 2 = 4.87, P 0.05.CSCC). The expression of Syndecan-1 was negatively correlated with the expression of HPA-1, and the expression of rs=-0.473,P0.01. was negatively correlated with the decrease of histological grade (s =-0.793 and-0.718, P 0.001). Conclusion the decreased expression of Syndecan-1 and increased expression of HPA-1 may promote the invasion and metastasis of CSCC.
【作者单位】： 潍坊市人民医院皮肤科;潍坊市人民医院病理科;
【基金】：潍坊市科技发展计划(201302005)
【分类号】：R739.5
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