嗜酸性粒细胞增多性皮炎患者外周血中IgE水平和嗜酸性粒细胞计数相关性研究

发布时间：2019-04-18 12:37

【摘要】：目的:本课题旨在检测嗜酸性粒细胞增多性皮炎(hypereosinophilic dermatitis,HED)患者外周血中免疫球蛋白E(immunoglobulin E,Ig E)的水平和嗜酸性粒细胞(eosinophil cell,EOS)计数的相关性,为HED的临床诊断及治疗方案的制定提供实验数据支持。方法:本课题严格按照纳入标准和排除标准,挑选了2014年1月-2016年1月间就诊于山西医科大学第一临床医学院皮肤性病科进行住院治疗的HED患者(组成病例组)30例及在同时期于山西医科大学第一临床医学院常规进行体检的体检者(组成对照组)30例,应用血球计数仪测定外周血中嗜酸性粒细胞的计数水平,应用酶联免疫吸附测定法(ELISA)测定其外周血中Ig E的水平。结果以EOS计数水平以大于1.5×109/L为异常检出,计做(+),以小于1.5×109/L为阴性检出,计做(-);Ig E水平其结果以大于120KU/L为异常检出,计做(+),以小于120KU/L为阴性检出,计做(-)。应用SPSS22.0统计软件进行分析:计量资料采用均数数据±标准差(X±S)表示,两组间计量资料的比较采用t检验进行分析,数据间相关性采用相关性分析,以P0.05表示差异具有统计学意义。结果:(1)病例组外周血中EOS计数水平和Ig E水平的异常检出率依次为57%、93%;对照组外周血中Ig E水平的异常检出率为3%。病例组的EOS计数高于对照组EOS计数,其差异具有显著性(t=3.502,P0.01)。病例组的Ig E计数高于对照组,其差异具有显著性(t=7.922,P0.01)。即病例组外周血中EOS计数和Ig E水平均高于对照组。(2)通过统计学方法分析得出病例组及对照组外周血中EOS计数水平与Ig E水平经过相关性分析呈相关性。病例组通过Spearman秩相关分析求得rs值为0.603,对照组通过Pearson相关性分析求得r值为0.697,并且两组均为中度正相关关系,二者在检测水平上的差别具有统计学意义(P0.05)。结论:检测得Ig E在HED患者的体内均呈高水平状态,且在HED患者中Ig E水平与EOS计数水平是呈中度正相关性。测定其值有助于HED的临床观察与治疗。
[Abstract]:Objective: to detect the correlation between the level of immunoglobulin (E (immunoglobulin E, IgE) and eosinophil (eosinophil cell,EOS) count in peripheral blood of patients with eosinophilic dermatitis (hypereosinophilic dermatitis,HED). To provide experimental data support for the clinical diagnosis and treatment plan of HED. Methods: the subject is strictly in accordance with the inclusion criteria and exclusion criteria. From January 2014 to January 2016, 30 HED patients who were hospitalized in the Department of Dermatology, first Clinical Medical College of Shanxi Medical University and the first clinical doctor of Shanxi Medical University during the same period were selected. There were 30 physical examiners (control group) who underwent routine physical examination in the college. The levels of eosinophils in peripheral blood and Ig E in peripheral blood were measured by blood cell counter and enzyme linked immunosorbent assay (ELISA) respectively. Results the abnormal detection level of EOS was more than 1.5 脳 109 渭 L, the negative detection of (), was less than 1.5 脳 109 渭 L, and the count was (-). The results of Ig E level were greater than 120KU/L as abnormal detection, (), was less than 120KU/L as negative detection, and (-) was counted as (-). SPSS22.0 statistical software was used to analyze: the measurement data was expressed by mean data 卤standard deviation (XS), the comparison of measurement data between the two groups was analyzed by t-test, and the correlation between data was analyzed by correlation analysis. The difference was statistically significant (P0.05). Results: (1) the abnormal detection rates of EOS count and Ig E in peripheral blood were 57% and 93% in the case group and 3% in the control group, respectively. The EOS count in the case group was higher than that in the control group, and the difference was significant (t = 3.502, P0.01). The Ig E count in the case group was significantly higher than that in the control group (t = 7.922, P0.01). That is to say, the EOS count and Ig E level in the peripheral blood of the case group were higher than those of the control group. (2) the correlation between the EOS count level and the Ig E level in the peripheral blood of the patient group and the control group was analyzed by statistical analysis. The rs of the case group was 0.603 by Spearman rank correlation analysis, and that of the control group was 0.697 by Pearson correlation analysis, and both of the two groups were moderate positive correlation. The difference in detection level between the two was statistically significant (P0.05). Conclusion: the level of Ig E is high in patients with HED, and there is a moderate positive correlation between the level of Ig E and the level of EOS in patients with HED. The determination of its value is helpful to the clinical observation and treatment of HED.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R758.2

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