铒激光点阵法嫩肤的动物实验观察
发布时间：2019-04-30 06:33
【摘要】：目的：1)观察Er:YAG点阵激光连续脉冲照射脱毛后的昆明种小鼠背部皮肤后,皮肤的即刻反应,及显微镜下小鼠真皮的厚度、胶原纤维及弹力纤维的变化。2)观察单位面积内脉冲数固定的情况下,提高激光照射能量后小鼠皮肤即刻反应及显微镜下真皮厚度、胶原纤维及弹力纤维的变化。 方法：随机取108只白色昆明种小鼠,雄性(清洁级)体重20g±3,8-10周龄平均分成三组(高能量照射组,低能量照射组,对照组),每组36只,在单位面积内脉冲数固定的情况下,利用点阵铒激光以两种不同能量(100J/cm2,25J/cm2连续脉冲照射脱毛后的昆明种小鼠背部皮肤,对照组不进行激光照射,按照射后1天,3天,7天,14天,28天,56天取实验组及对照组小鼠背部皮肤做病理切片测量真皮厚度的变化及特殊染色后观察胶原纤维和弹力纤维数量的改变。 结果：1)点阵铒激光照射昆明种小鼠皮肤后,皮肤即刻出现一系列紧密排列的局灶性点状汽化剥脱微损伤,能量高的照射组可见局灶性微损伤区轻度点状出血；照射后1-2天微损伤区皮肤发红,出现炎症反应,自3-4天开始炎症和坏死组织逐渐形成痂皮并脱落,6天内痂皮脱落完全；7天-56天显微镜下表皮层略有增厚,真皮层增厚明显,局部胶原纤维新生重塑,照射后14天开始真皮层增厚显著,且胶原纤维及弹力纤维一直持续处于增生状态,至56天时实验组真皮厚度,胶原纤维及弹力纤维含量仍明显高于对照组。2)采用不同能量激光照射小鼠背部皮肤后可见高能量实验组较低能量实验组真皮厚度增加更为明显,纤维组织增生较为明显,但愈合时间也相应略有延长,表明在一定能量范围内,随着点阵激光照射能量的不断增加,其产生的治疗效果也相应增加,但副作用也可能相继出现。 结论：点阵铒激光照射昆明种小鼠背部皮肤后可有效的刺激真皮胶原纤维及弹力纤维增生,增加真皮层厚度,从而增强皮肤弹性,达到嫩肤效果,且愈合较为迅速。在一定范围内,随激光照射能量的增加,真皮层厚度及胶原纤维、弹力纤维数量将增加。
[Abstract]:Objective: 1) to observe the immediate reaction of the back skin of Kunming mice and the thickness of mouse dermis under microscope after Er:YAG lattice laser continuous pulse irradiation on the back skin of Kunming mice after depilation. The changes of collagen fiber and elastic fiber were observed. 2) under the condition of fixed pulse number per unit area, the immediate reaction of skin and the changes of dermal thickness, collagen fiber and elastic fiber under microscope after laser irradiation were increased. Methods: 108 white Kunming mice were randomly divided into three groups (high energy irradiation group, low energy irradiation group, control group), with 36 mice in each group under the condition of constant pulse number per unit area, male (clean grade) weight 20g 卤3,8 weeks old. Two different energies (100J / cm ~ 2, 25J / cm ~ 2) were used to irradiate the back skin of Kunming mice with two different energies (100J / cm ~ 2, 25J / cm ~ 2). In the control group, the skin of Kunming mice was irradiated at 1, 3, 7, 14, 28 days after irradiation without laser irradiation. The thickness of the dermis and the number of collagen fibers and elastic fibers were measured by pathological sections of the skin of the experimental group and the control group on the 56th day. Results: 1) when Kunming mice skin was irradiated by dot-array erbium laser, a series of focal punctate vaporization and denudation injury occurred immediately, and slight punctate hemorrhage could be seen in the high energy irradiation group. 2 days after irradiation, the skin was red and appeared inflammatory reaction. From 3 to 4 days, the inflammation and necrosis tissue gradually formed and exfoliated, and the skin exfoliated completely within 6 days. From 7 days to 56 days after irradiation, the epidermis was slightly thickened and the epidermis was thickened obviously, and the local collagen fibers were reconstituted. On the 14th day after irradiation, the dermal cortex became thickened significantly, and the collagen fibers and elastic fibers continued to proliferate. At 56 days, the dermal thickness, collagen fiber and elastic fiber contents in the experimental group were still significantly higher than those in the control group. 2) after irradiated with different energy laser, the dermal thickness in the high energy group was more obvious than that in the low energy group. The fibrous tissue hyperplasia was obvious, but the healing time was slightly prolonged, indicating that in a certain energy range, with the increasing energy of lattice laser irradiation, the therapeutic effect also increased, but the side effects might also appear one after another. Conclusion: the lattice erbium laser irradiation on the back of Kunming mice can effectively stimulate the proliferation of dermal collagen fibers and elastic fibers and increase the thickness of dermis so as to enhance the elasticity of the skin and achieve the effect of skin rejuvenation, and the healing is more rapid. In a certain range, with the increase of laser irradiation energy, the thickness of dermis and the number of collagen fibers and elastic fibers will increase.
【学位授予单位】：大连医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R751.05
本文编号：2468560
[Abstract]:Objective: 1) to observe the immediate reaction of the back skin of Kunming mice and the thickness of mouse dermis under microscope after Er:YAG lattice laser continuous pulse irradiation on the back skin of Kunming mice after depilation. The changes of collagen fiber and elastic fiber were observed. 2) under the condition of fixed pulse number per unit area, the immediate reaction of skin and the changes of dermal thickness, collagen fiber and elastic fiber under microscope after laser irradiation were increased. Methods: 108 white Kunming mice were randomly divided into three groups (high energy irradiation group, low energy irradiation group, control group), with 36 mice in each group under the condition of constant pulse number per unit area, male (clean grade) weight 20g 卤3,8 weeks old. Two different energies (100J / cm ~ 2, 25J / cm ~ 2) were used to irradiate the back skin of Kunming mice with two different energies (100J / cm ~ 2, 25J / cm ~ 2). In the control group, the skin of Kunming mice was irradiated at 1, 3, 7, 14, 28 days after irradiation without laser irradiation. The thickness of the dermis and the number of collagen fibers and elastic fibers were measured by pathological sections of the skin of the experimental group and the control group on the 56th day. Results: 1) when Kunming mice skin was irradiated by dot-array erbium laser, a series of focal punctate vaporization and denudation injury occurred immediately, and slight punctate hemorrhage could be seen in the high energy irradiation group. 2 days after irradiation, the skin was red and appeared inflammatory reaction. From 3 to 4 days, the inflammation and necrosis tissue gradually formed and exfoliated, and the skin exfoliated completely within 6 days. From 7 days to 56 days after irradiation, the epidermis was slightly thickened and the epidermis was thickened obviously, and the local collagen fibers were reconstituted. On the 14th day after irradiation, the dermal cortex became thickened significantly, and the collagen fibers and elastic fibers continued to proliferate. At 56 days, the dermal thickness, collagen fiber and elastic fiber contents in the experimental group were still significantly higher than those in the control group. 2) after irradiated with different energy laser, the dermal thickness in the high energy group was more obvious than that in the low energy group. The fibrous tissue hyperplasia was obvious, but the healing time was slightly prolonged, indicating that in a certain energy range, with the increasing energy of lattice laser irradiation, the therapeutic effect also increased, but the side effects might also appear one after another. Conclusion: the lattice erbium laser irradiation on the back of Kunming mice can effectively stimulate the proliferation of dermal collagen fibers and elastic fibers and increase the thickness of dermis so as to enhance the elasticity of the skin and achieve the effect of skin rejuvenation, and the healing is more rapid. In a certain range, with the increase of laser irradiation energy, the thickness of dermis and the number of collagen fibers and elastic fibers will increase.
【学位授予单位】：大连医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R751.05
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