FHIT、hTERT蛋白在皮肤鳞状细胞癌中的表达及意义

发布时间：2019-05-01 07:33

【摘要】：目的：通过检测脆性组氨酸三联体(FHIT)及人类端粒酶逆转录酶(hTERT)蛋白在皮肤鳞状细胞癌(SCC)、鲍温病(BD)、日光角化病(SK)及正常对照皮肤组织中的表达情况,探讨FHIT及hTERT蛋白与SCC发生及演进的关系。方法：①收集经病理确诊的50例SCC、20例BD及15例SK的蜡块,10例对照皮肤的蜡块,均进行连续切片。②采用免疫组化PV-6000法检测上述组织中FHIT和hTERT蛋白的表达。比较SCC、SK、BD及对照皮肤之间hTERT及FHIT的表达情况。结果：①FHIT在对照组、SK组、BD组及SCC组的表达阳性率分别为100.0%、73.3%、55.0%和44.0%,FHIT蛋白的表达在对照组和BD组、SCC组之间(X2=6.429、10.500),SK组和SCC组之间(X2=3.972),高分化组和中低分化组之间(X2=4.020),无淋巴结转移组和有淋巴结转移组之间(X2=4.788),差异均有显著性(p均＜0.05)。FHIT在对照组皮肤均为阴性,在SK组、BD组及SCC组的表达阳性率分别为40.0%、70.0%和80.0%,hTERT蛋白的表达在对照组和SK组、BD组和SCC组之间均有显著性差异(p均＜0.05),在SK组和SCC组之间(X2=8.924),高分化组和中低分化组之间(X2=4.861),无淋巴结转移组和有淋巴结转移组之间(X2=3.947),差异也均有显著性(p均＜0.05),在SK组与BD组和SCC组之间,差异均无显著性(p均＞0.05)。③在SCC组中FHIT阳性率、阳性强度越高,其hTERT的表达阳性率和阳性强度越低。相关性分析结果也表明,SCC组中FHIT蛋白的阳性表达与hTERT蛋白的阳性表达呈负相关(r=-0.363,p=0.010)。结论： FHIT蛋白的表达减少与皮肤鳞状细胞癌的发生及演进有关；hTERT蛋白的表达增多与皮肤鳞状细胞癌的发生及演进有关；FHIT和hTERT在皮肤鳞状细胞癌发生、演进过程中发挥拮抗作用。
[Abstract]:Objective: to detect the expression of fragile histidine triad (FHIT) and human telomerase reverse transcriptase (hTERT) protein in skin squamous cell carcinoma (SCC), (SCC), Bowen's disease (BD), daylight keratosis (SK) and normal skin tissues. To investigate the relationship between FHIT and hTERT proteins and the genesis and progression of SCC. Methods: 1Wax blocks were collected from 50 cases of SCC,20, 15 cases of SK and 50 cases of BD, and 10 cases of normal skin were sectioned continuously. 2 Immunohistochemistry PV-6000 method was used to detect the expression of FHIT and hTERT protein in the above tissues. To compare the expression of hTERT and FHIT between SCC,SK,BD and control skin. Results: the positive rates of 1FHIT expression in control group, SK group, BD group and SCC group were 100. 0%, 73. 3%, 55. 0% and 44. 0%, respectively. The expression of Fhit protein was between control group and BD group and SCC group (X 2, 6. 429, 10. 500). Between the SK and SCC groups (X2, 3.972), between the well-differentiated and low-differentiated groups (X2, 4.020), between the non-lymph node metastasis group and the lymph node metastasis group (X2, 4.788), and between the high-differentiated group and the low-and middle-differentiated group (X2, 4.020). The positive rates of Fhit in SK group, BD group and SCC group were 40.0%, 70.0% and 80.0%, respectively, and the expression of hTERT protein was in control group and SK group, and the expression of Fhit protein was negative in the control group and SK group, and the positive rate was 40.0%, 70.0% and 80.0% in the control group, BD group and SCC group, respectively. There was a significant difference between BD group and SCC group (P < 0 05), between SK group and SCC group (X2, 8. 924), between well-differentiated group and low-and middle-differentiated group (X2, 4.861), and there was no significant difference between the two groups (P < 0. 05). There was also a significant difference between the group without lymph node metastasis and the group with lymph node metastasis (P < 0 05). Between SK group, BD group and SCC group, there was significant difference between the two groups (P < 0 05), and the difference was significant between the two groups (P < 0 05). 3 in SCC group, the higher the positive rate and intensity of FHIT, the lower the positive rate and intensity of hTERT. The results of correlation analysis also showed that the positive expression of FHIT protein was negatively correlated with the positive expression of hTERT protein in SCC group (r = 0.363, p = 0.010). Conclusion: the decreased expression of FHIT protein is related to the carcinogenesis and progression of cutaneous squamous cell carcinoma, and the increased expression of hTERT protein is related to the carcinogenesis and progression of cutaneous squamous cell carcinoma. FHIT and hTERT play an antagonistic role in the development and progression of cutaneous squamous cell carcinoma.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R739.5

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