扁平苔藓皮损中IL-17、FoxP3的表达及意义

发布时间：2019-06-03 14:09

【摘要】：目的：检测扁平苔藓(Lichen Planus,LP)皮损中白介素17(IL-17)和叉头转录因子3(FoxP3)的表达,探讨Th17细胞和Treg细胞的相关炎症因子失衡及细胞凋亡与扁平苔藓的关系。方法：采用免疫组化法检测30例扁平苔藓患者皮损和30例健康人皮肤组织中IL-17、FoxP3的表达情况,用原位末端标记技术(TUNEL)检测皮损处细胞凋亡情况,并进行水平相关性的分析。结果：扁平苔藓组IL-17和FoxP3的积分光密度值分别为14670±1790和14520±2560,均明显高于健康对照组(分别为7210±1210、7090±1050),两组比较,t值分别为18.88、14.69,P值均小于0.01;扁平苔藓组角质形成细胞凋亡指数(70.55±5.78),明显高于健康对照组(30.01±3.97),两组比较,t=31.67,P0.01。扁平苔藓组IL-17、FoxP3呈正相关,IL-17、FoxP3与角质形成细胞凋亡程度呈正相关。结论：(1)扁平苔藓皮损中的IL-17、FoxP3高表达,提示其参与了扁平苔藓的发病。(2)扁平苔藓皮损中存在角质形成细胞的凋亡亢进。(3)扁平苔藓皮损中IL-17、FoxP3的表达呈正相关,且两者在皮损中的表达均与角质形成细胞凋亡指数呈正相关,提示在扁平苔藓皮损中IL-17、FoxP3介导的炎症反应与细胞凋亡程度具有一定的相关性。
[Abstract]:Objective: To investigate the expression of interleukin-17 (IL-17) and transcription factor 3 (FoxP3) in the skin of Lichen Planus (LP), and to explore the relationship between the imbalance of the relevant inflammatory factors and the cell apoptosis and the lichen planus of Th17 cells and Treg cells. Methods: The expression of IL-17 and FoxP3 in 30 patients with lichen planus and 30 healthy controls were detected by immunohistochemistry, and the cell apoptosis was detected by TUNEL in situ. Results: The integral optical density of the levels of IL-17 and FoxP3 in the lichen planus was 14670,1790 and 14520-2560, respectively, which was significantly higher than that in the healthy control group (7210-1210,7090-1050). The t-values were 18.88, 14.69 and P <0.01, respectively. Compared with the healthy control group (30.01-3.97), the two groups were compared, t = 31.67, P0.01. There was a positive correlation between the levels of IL-17 and FoxP3 in the lichen planus, and the levels of IL-17 and FoxP3 were positively correlated with the degree of apoptosis. Conclusion: (1) The expression of IL-17 and FoxP3 in the skin of the lichen planus indicates that it is involved in the development of the lichen planus. (2) In the skin of the lichen planus, there is a hyperfunction of the keratinocytes in the skin of the lichen planus. (3) The expression of IL-17 and FoxP3 in the skin of the lichen planus was positively correlated, and the expression of the two in the lesions was positively correlated with the apoptosis index of the keratinocytes, suggesting that the inflammatory response mediated by IL-17 and FoxP3 in the skin of the lichen planus has a certain correlation with the degree of apoptosis.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R758.65

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