P53抑制剂和微管抑制剂对银屑病表皮角质形成细胞糖皮质激素受体核转运的影响

发布时间：2019-06-18 10:40

【摘要】：目的:探讨P53抑制剂和微管抑制剂对银屑病表皮角质形成细胞糖皮质激素受体(GR)核转运的影响。方法:分离和培养银屑病患者和正常人表皮角质形成细胞,分别与P53抑制剂和微管蛋白抑制剂共培养,加或不加血管内皮细胞生长因子(VEGF),间接免疫荧光法检测上述因素对GR核转位的影响。结果:VEGF可诱导正常角质形成细胞发生核转位;P53抑制剂抑制了VEGF诱导的GR核转位。与VEGF共培养的角质形成细胞的核转位评分显著低于单纯角质形成细胞的核转运评分,差异有统计学意义(P0.05);微管抑制剂可完全将正常表皮角质形成细胞的GR滞留在胞浆中,在加入VEGF后,与单纯微管抑制剂组比较,胞浆中GR并没有明显增多;而微管抑制剂和P53抑制剂共培养,会有少量GR进入角质形成细胞的胞核中。结论:微管介导银屑病角质形成细胞GR的核摄取,P53参与了GR的核输出。
[Abstract]:Objective: To study the effect of P53 inhibitor and microtubule inhibitor on the expression of glucocorticoid receptor (GR) in the epidermal keratinocytes of psoriasis. Methods: The effects of the above factors on the nuclear translocation of GR were detected by co-culture with P53 inhibitor and microtubule protein inhibitor, with or without vascular endothelial growth factor (VEGF) and indirect immunofluorescence. Results: VEGF can induce the nuclear translocation of normal keratinocytes; the inhibitor of P53 inhibits the VEGF-induced GR-nuclear translocation. The nuclear translocation score of the keratinocytes co-cultured with VEGF was significantly lower than that of the pure cutin-forming cells, and the difference was significant (P0.05); the microtubule inhibitor could completely hold the GR of the normal epidermal keratinocytes in the cytoplasm, and after the addition of VEGF, In comparison with the simple microtubule inhibitor group, there was no significant increase in GR in the cytoplasm, while the microtubule inhibitor and the P53 inhibitor co-cultured with a small amount of GR into the cytoplasm of the keratinocytes. Conclusion: Microtubules mediate the nuclear uptake of GR, and P53 is involved in the nuclear output of GR.
【作者单位】：台州市肿瘤医院皮肤科;台州中西医结合医院皮肤科;台州市肿瘤医院药剂科;
【分类号】：R758.63

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