BIGLYCAN在光老化中的作用机制研究

发布时间：2021-02-25 13:17

　　研究背景：二聚糖（Biglycan, BGN）是一种属于糖蛋白类的蛋白质,由42kDa的核心蛋白（core protein）和两个硫酸软骨素／硫酸皮肤素（chondroitin sulfate, CS/dermatan sulfate, DS）糖胺聚糖（glycosaminoglycan, GAG）链组成,在皮肤组织结构合成、空间填充和皮肤生理功能中起到重要的作用。虽然许多组织表达丰富的二聚糖,但其生物功能仍然知之甚少。二聚糖核心蛋白的合成起始于内质网（endoplasmic reticulum, ER）,并继续转移至高尔基体,通过各种糖基转移酶（glycosyltransferases, GTs）的作用下合成CS/DS-GAG链。二聚糖具有组建细胞外基质（extracellular matrix, ECM）的作用,靶向敲除二聚糖基因可以观察到异常胶原纤维形态和骨质疏松症样表型。分泌的二聚糖通过其核心蛋白和GAG侧链的共同作用下参与合成ECM,特别是对Ⅰ、 Ⅱ、Ⅲ、Ⅵ型胶原蛋白和弹力蛋白的装配起到重要的作用。最近研究发现二聚糖与炎症密切相关,在脂多糖（lipopolysaccharid... 

【文章来源】：延边大学吉林省 211工程院校

【文章页数】：89 页

【学位级别】：博士

【文章目录】：
中文摘要
ABSTRACT
ABBREVIATIONS
PART ONE
    1. Introduction
    2. Materials and methods
        2.1 Cell culture and UV irradiation
        2.2 Quantitative real-time reverse transcription-polymerase chain reaction（RT-PCR）
        2.3 Gene silencing with siRNA
        2.4 Overexpression of wild-type and mutant BGN proteins in primary human dermalfibroblasts
        2.5 Western blot analysis
        2.6 Statistics
    3. Results
        3.1 UV irradiation increased defectively-glycosylated BGN expression dose-dependently incultured human dermal fibroblasts
        3.2 UV-induced mRNA expression changes of xylosyltransferases（XYLTs） in cultured humandermal fibroblasts
        3.3 Downregulation of XYLT1 by siRNA transfection impairs BGN GAG synthesis incultured human dermal fibroblasts
        3.4 Another DS proteoglycan,DCN,showed a little or no decrease of its molecular weight byUV irradiation and XYLT1 siRNA transfection in cultured human dermal fibroblasts
        3.5 XYLT1 can add xylose on S42 and S47 on the core protein in cultured human dermalfibroblasts,while XYLT2 can add xylose only on S42,but not S47
    4. Discussion
    5. Conclusions
PART TWO
    1. Introduction
    2. Materials and Methods
        2.1 Cell culture and UV irradiation
        2.2 UV and LPS stimulation
        2.3 Quantitative real-time reverse transcription-polymerase chain reaction（RT-PCR）
        2.4 Gene silencing with siRNA
        2.5 Statistics
    3. Results
        3.1 Down-Regulation of BGN prevents UV-induced IL-6,IL-8 gene expression in humanfibroblasts
        3.2 Down-Regulation of TLR4 prevents UV-induced IL-6,IL-8 gene expression in humanfibroblasts
        3.3 Down-Regulation of BGN and TLR4 prevents LPS-induced IL-6,IL-8 gene expression inhuman fibroblasts
    4. Discussion
    5. Conclusions
References
综述
    参考文献
致谢



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