丘脑底核深部脑刺激改善帕金森病模型大鼠自发跑轮行为的研究

发布时间：2018-01-14 19:39

本文关键词：丘脑底核深部脑刺激改善帕金森病模型大鼠自发跑轮行为的研究　出处：《立体定向和功能性神经外科杂志》2016年04期 　论文类型：期刊论文

【摘要】：目的探讨丘脑底核(subthalamic nucleus,STN)深部脑刺激(deep brain stimulation,DBS)能否改善6-羟基多巴胺偏侧帕金森病模型大鼠自发跑轮行为。方法将35只Sprague-Dawley(SD)大鼠随机分为实验组24只和对照组11只。利用立体定向的方法将刺激电极埋入右侧丘脑底核。实验组大鼠右侧内侧前脑束内注射12μg 6-OHDA,对照组注射相同体积的生理盐水。对造模前、造模后和给予电刺激时的大鼠进行前肢使用不对称测试和自发跑轮行为测试。酪氨酸羟化酶免疫组化染色检测黑质多巴胺能神经元毁损程度。苏木素-伊红染色确定刺激电极尖端位置。结果从24只大鼠中获得了17只成功的PD大鼠模型。前肢使用不对称测试:与毁损后未给予STN-DBS时比较,电极尖端位于STN内的成功模型大鼠给予STN-DBS后,不对称指数显著下降(P=0.038),而与毁损前相比,差异不显著(P=0.07)。跑轮行为测试:与毁损后未给予STN-DBS时比较,电极尖端位于STN内的成功模型大鼠给予STN-DBS后,跑动次数(P=0.032)、跑动距离(P=0.03)和平均峰速度均(P=0.035)显著增加,而从跑动开始到峰速度的平均时间显著减小(P=0.001)。这些指标与毁损前比较,差异均无统计学意义。结论丘脑底核深部脑刺激能够改善6-羟基多巴胺偏侧帕金森病模型大鼠的自发跑轮行为,自发跑轮行为测试在一定程度上可用于筛选抗帕金森病药物或其他治疗方法。
[Abstract]:Objective to investigate the deep brain stimulation of the subthalamic nucleus subthalamic nucleus (STN). Methods 35 SD rats with Sprague-Dawley disease were treated with DBS to improve the spontaneous wheel running behavior of 6-hydroxydopamine hemiparkinsonism model rats. Rats were randomly divided into experimental group (n = 24) and control group (n = 11). The stimulation electrode was implanted into the right subthalamic nucleus by stereotactic method. In the experimental group, 12 渭 g 6-OHDA was injected into the right medial forebrain tract. The control group was injected with the same volume of normal saline. The antecedent limb was tested by asymmetrical test and spontaneous wheel running behavior test after modeling and electrical stimulation. Tyrosine hydroxylase immunohistochemical staining was used to detect the damage degree of substantia nigra dopaminergic neurons. Hematoxylin-eosin staining. Results 17 successful PD rat models were obtained from 24 rats. The results were compared with those when STN-DBS was not given after damage. After STN-DBS was given to the successful model rats with the tip of the electrode located in STN, the asymmetry index decreased significantly (P < 0.05), compared with that before STN-DBS. Test of running wheel behavior: compared with those without STN-DBS, the successful model rats with tip of electrode located in STN were given STN-DBS. The running times (P0. 032), the running distance (P0. 03) and the average peak velocity (P0. 035) increased significantly. However, the average time from the beginning of running to the peak velocity decreased significantly. These indexes were compared with those before damage. Conclusion the deep brain stimulation of subthalamic nucleus can improve the spontaneous wheel running behavior of 6-hydroxydopamine hemiparkinsonism model rats. Spontaneous wheel-running behavior testing can be used to screen antiParkinson disease drugs or other treatment methods to some extent.
【作者单位】：海南医学院附属医院神经外科;南方医科大学珠江医院神经外科;青海大学附属医院神经外科;北京中国科学院心理研究所;陆军总医院附属八一脑科医院;
【基金】：国家自然科学基金项目(编号:30872662) 海南医学院科研培育基金项目(编号:HY2015-19)
【分类号】：R-332;R742.5
【正文快照】： 作者单位:570102海口海南医学院附属医院神经外科(潘琪);南方医科大学珠江医院神经外科(张旺明),青海大学附属医院神经外科(肖宗宇),北京中国科学院心理研究所(罗非),陆军总医院附属八一脑科医院(徐如祥)深部脑刺激(deep brain stimulation,DBS)是借助立体定向手术,将刺激电极

【相似文献】