缺血后适应对大鼠局灶性脑缺血再灌注所致自噬的抑制作用

发布时间：2018-01-25 01:19

  本文关键词： 脑 缺血再灌注损伤 缺血后适应 自噬 哺乳动物雷帕霉素靶蛋白　出处：《中国病理生理杂志》2017年10期 　论文类型：期刊论文

【摘要】：目的:探讨缺血后适应对大鼠局灶性脑缺血再灌注所致自噬的影响。方法:取健康雄性SD大鼠30只,随机分为假手术(sham)组、缺血再灌注(I/R)组、缺血后适应(IPC)组,每组各10只。Sham组仅单纯暴露右侧颈总、颈内和颈外动脉;I/R组采用Longa改良线栓法制备大鼠右侧大脑中动脉缺血2 h、再灌注24 h模型;IPC组大鼠缺血2 h后,同侧颈总动脉再通10 s/闭塞10 s,循环5次,然后全面恢复血流再灌注24 h。采用透射电镜观察脑细胞自噬情况;Western blot法测定各组大鼠脑组织中哺乳动物雷帕霉素靶蛋白(m TOR)、磷酸化m TOR(pm TOR)和微管相关蛋白轻链3(LC3)-Ⅱ的蛋白表达水平;TTC法检测脑梗死面积;干湿称重法测定脑组织含水量;HE染色观察脑组织病理学变化。结果:IPC组m TOR、p-m TOR均显著高于I/R组(P0.05),LC3-Ⅱ显著低于I/R组(P0.01)。IPC组脑梗死面积、脑组织含水量均显著低于I/R组(P0.01)。HE染色显示,IPC组神经元变性、坏死较I/R组明显减轻。透射电镜显示IPC组神经元损伤程度明显减轻,自噬泡明显减少。结论:IPC通过减少细胞内自噬而减轻脑缺血再灌注损伤,可能与增强m TOR作用有关。
[Abstract]:Objective: to investigate the effect of ischemic adaptation on autophagy induced by focal cerebral ischemia reperfusion in rats. Methods: thirty healthy male SD rats were randomly divided into sham group. 10 rats in each group were exposed to the right total carotid artery, internal carotid artery and external carotid artery. In the I / R group, the right middle cerebral artery was ischemia for 2 h and reperfusion for 24 h. After ischemia for 2 hours in IPC group, the ipsilateral common carotid artery was recanalized for 10 s / 10 s, followed by 5 circulations, and then the blood flow was recovered completely for 24 h. The autophagy of brain cells was observed by transmission electron microscope (TEM). The mammalian rapamycin target protein (rapamycin) was determined by Western blot method. The protein expression level of phosphorylated m TOR(pm word) and microtubule-associated protein light chain 3C3F- 鈪，

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