低氧预处理降低大鼠癫痫易感性及其脑保护作用的机制初探

发布时间：2018-01-25 08:37

本文关键词： 低氧预处理癫痫脑保护大鼠　出处：《中国比较医学杂志》2016年12期 　论文类型：期刊论文

【摘要】：目的初步探讨间断低氧预处理(intermittent hypoxia preconditioning,IHP)对氯化锂-匹鲁卡品(lithium-pilocarpine,Li-pilo)致痫大鼠痫性发作的影响及其脑保护作用机制。方法 96只清洁级SD大鼠,随机均分为对照组,癫痫组和4个间断低氧预处理+癫痫组。癫痫组及4个间断低氧预处理+癫痫组(分别在5 d IHP预处理后1,3,7,14日注射)大鼠通过腹腔注射氯化锂-匹鲁卡品建立癫痫模型。随后进行240 min的发作行为学,全身性发作潜伏期及百分比量化分析并通过水迷宫实验测试大鼠认知功能。接着利用TUNEL标记和免疫印迹方法进行大鼠海马神经元凋亡及相关蛋白(BCL-2,Bax和cleaved-caspase-3)的检测。结果氯化锂-匹鲁卡品注射后50~150 min之间,癫痫发作的改良Racine评分达到峰值。间断低氧预处理后3日诱发癫痫组大鼠其平均改良Racine评分明显低于其余各组,该组全身性发作潜伏期及百分比与癫痫组相比差异亦有统计学意义(P0.05)。相比癫痫组和其余3个间断低氧预处理+癫痫组大鼠,间断低氧预处理后3日诱发癫痫组大鼠逃避潜伏期(escape latency)较短,神经元凋亡计数较低,而目标象限停留时间百分比较高(P0.05)。间断低氧预处理后3日诱发癫痫组的水迷宫以及凋亡检测参数与对照组相比较无明显差异(P0.05)。结论 IHP预处理通过抑制异常凋亡降低大鼠癫痫易感性,并具有脑保护作用。
[Abstract]:Objective to investigate the intermittent hypoxic preconditioning hypoxia preconditioning. The lithium-pilocarpine of lithium-pilocarpine was determined by IHP. Methods 96 SD rats of clean grade were randomly divided into control group. Epilepsy group and 4 intermittent hypoxic preconditioning epilepsy group, epilepsy group and 4 intermittent hypoxic preconditioning epilepsy group (5 days after IHP preconditioning 1 3) 7. In 14th, rats were injected with lithium-pilocarpine intraperitoneally to establish an epileptic model, and then the seizure behavior was performed for 240 min. The latency and percentage of systemic seizures were quantitatively analyzed and the cognitive function of rats was tested by water maze test. Then the apoptosis and related proteins of hippocampal neurons were detected by TUNEL labeling and Western blotting. BCL-2. Bax and cleaved-caspase-3). Results Lithium-Pilucapine was injected for 50 ~ 150 min. The mean modified Racine score of epileptic seizures group was significantly lower than that of other groups after intermittent hypoxic preconditioning on 3rd. The latency and percentage of systemic seizures in this group were significantly different from those in the epileptic group (P 0.05), compared with the epileptic group and the other 3 intermittent hypoxic preconditioning groups. After intermittent hypoxia preconditioning on 3rd rats induced epilepsy group escape latencies were shorter and the number of neuronal apoptosis was lower. However, the percentage of the target quadrant residence time was higher than that of the control group (P 0.05). There was no significant difference in the water maze and apoptotic parameters in the epileptic group induced by intermittent hypoxia preconditioning on 3rd compared with the control group (P 0.05). Conclusion IHP preconditioning can inhibit abnormal apoptosis and decrease the susceptibility to epilepsy in rats. And has the brain protection function.
【作者单位】：兰州军区兰州总医院安宁分院;兰州军区兰州总医院;
【分类号】：R742.1
【正文快照】： induced seizure peaked on an average between 50-150 min after Li-pilo administration,scored using a modified Racinescale.The average scores of modified Racine scale in the IHP-3d seizure group was significantly lower than that in the othergroups.The late

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