转录因子FOXC1促进胶质瘤细胞系U87的侵袭能力

发布时间：2018-01-27 16:15

本文关键词： 胶质瘤 FOXC 侵袭　出处：《南京医科大学学报(自然科学版)》2017年05期 　论文类型：期刊论文

【摘要】：目的:探讨转录因子FOXC1对人脑胶质瘤细胞系U87侵袭的促进作用。方法:qPCR、Western blot检测胶质瘤细胞系U87、U251及正常星型胶质细胞中FOXC1 mRNA和蛋白表达水平。将FOXC1-siRNA转染至U87细胞中,分别用qPCR、Western blot检测FOXC1-siRNA的转染效率。细胞划痕实验和Transwell实验检测U87细胞转染FOXC1-siRNA后对细胞侵袭能力的影响,利用Western blot检测U87细胞上皮间质化相关通路蛋白(Snail1、β-catenin、Twist1)、标记蛋白(N-cadherin、Vimentin、E-cadherin)表达水平。结果 :U87细胞下调FOXC1表达后侵袭能力明显减弱,上皮间质化相关通路蛋白(Snail1、β-catenin、Twist1)表达水平下降,上皮细胞标记蛋白表达水平E-cadherin表达水平升高、间质细胞标记蛋白N-cadherin、Vimentin表达水平降低。结论:转录因子FOXC1通过上皮间质化促进胶质瘤细胞的侵袭。
[Abstract]:Objective: to investigate the effect of transcription factor FOXC1 on the invasion of human glioma cell line U87. The expression levels of FOXC1 mRNA and protein in U251 and normal astrocytes were detected. FOXC1-siRNA was transfected into U87 cells with qPCR. Western. Blot was used to detect the transfection efficiency of FOXC1-siRNA. Cell scratch assay and Transwell assay were used to detect the invasion ability of U87 cells transfected with FOXC1-siRNA. Influence. Western blot was used to detect the epithelial interstitial pathway proteins (Snail1, 尾 -cateninine Twist1) in U87 cells. The expression level of N-cadherin in Vimentinnus E-cadherin. Results after down-regulation of FOXC1 expression, the invasion ability of U87 cells was significantly decreased. The expression level of Snail1, 尾 -catenin Twist1 was decreased. The expression level of E-cadherin increased in epithelial cells, and N-cadherin in interstitial cells. Conclusion: transcriptional factor FOXC1 promotes the invasion of glioma cells through epithelialization.
【作者单位】：南京医科大学第一附属医院神经外科;
【基金】：国家自然科学基金(81201978) 江苏省科技厅基础研究计划(BK2012483)
【分类号】：R739.41
【正文快照】： 胶质瘤是成人中枢神经系统中最常见的恶性肿瘤,肿瘤细胞快速侵袭临近的正常脑组织是其治疗失败的一个主要原因。尽管经过手术治疗,术后辅以放疗化疗,胶质瘤患者的预后依然不佳,其平均生存期不足1年,复发率及致死率极高[1]。因此急需寻找新的治疗方法来改善胶质瘤患者的预后。

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