强制性运动疗法对老年脑卒中大鼠神经功能和碱性成纤维生长因子表达的影响

发布时间：2018-01-27 12:57

本文关键词： 强制性运动疗法脑卒中神经功能碱性成纤维生长因子　出处：《中国老年学杂志》2017年14期 　论文类型：期刊论文

【摘要】：目的探讨强制性运动疗法(CIMT)对老年脑卒中大鼠神经功能和碱性成纤维生长因子(b FGF)表达的影响。方法将90只SD大鼠按照随机数字表法分为CMIT组、常规康复组及对照组,每组30只,采用Zea Longa线栓法制备脑卒中模型,给予CMIT组以固定健侧上肢,强迫患侧肢体进行滚筒、平衡木、网屏训练;常规康复组以不限制健侧上肢进行滚筒、平衡木、网屏训练,训练时间与CIMT相同,每天训练时间上下午各15 min;对照组无任何训练,21 d后训练停止,各组大鼠饲养条件相同,实验干预7、14、21 d对大鼠进行行为功能(滚筒训练评分、平衡木测试评分、网屏抓握测试评分)及神经功能评定;采用real-time PCR方法检测b FGF、表皮生长因子(EGF)的mRNA表达,Western印迹法检测b FGF、EGF的蛋白水平。结果 CMIT组、常规康复组康复训练后行为功能及神经功能评分均明显降低(P0.05),且CMIT组21 d后行为功能评分,14、21 d神经功能评分均低于常规康复组(P0.05);CMIT组和常规康复组在康复训练7、14、21 d,b FGF和EGF蛋白及mRNA表达均高于对照组(P0.05),CMIT组在康复训练14、21 d,b FGF和EGF蛋白水平及mRNA表达均高于常规康复组(P0.05)。结论强制性运动疗法与康复训练相比,可以更好地促进出血性脑卒中大鼠肢体功能的恢复,其机制可能与促进b FGF和EGF表达有关。
[Abstract]:Objective to investigate the effects of compulsory exercise therapy (CIMT) on neurologic function and basic fibroblast growth factor (bFGFs) in aged rats with stroke. Methods 90 SD rats were divided into CMIT group according to random digital table method. The normal rehabilitation group and control group, 30 rats in each group, were treated with Zea Longa method to make stroke model. The normal upper limb was fixed in CMIT group, and the affected limb was forced to carry out roller and balance beam. Screen training; In the routine rehabilitation group, the roller, balance beam and screen training were performed on the upper limb without restriction. The training time was the same as that of CIMT, and the training time was 15 mins and 15 mins each day. In the control group, the training stopped after 21 days without any training, and the feeding conditions of each group were the same. The rats were given behavioral function (roller training score, balance beam test score). Screen grip test score) and neurological function evaluation; Real-time PCR was used to detect the expression of bFGFand EGF to detect the expression of mRNA. Western blot was used to detect the expression of b FGF. Results after rehabilitation training, the scores of behavioral function and neurological function in CMIT group and routine rehabilitation group were significantly decreased (P 0.05), and the scores of behavioral function in CMIT group were 21 days later. The neurological function scores were lower than those in the routine rehabilitation group on the 21st day (P 0.05). The expression of FGF, EGF protein and mRNA in CMIT group and routine rehabilitation group were higher than those in control group (P 0.05). The patients in CMIT group were treated with rehabilitation training for 14 days and 21 days. The protein levels of b FGF and EGF and the expression of mRNA were higher than those of routine rehabilitation group (P 0. 05). Conclusion compulsory exercise therapy is compared with rehabilitation training. It can promote the recovery of limb function in hemorrhagic stroke rats, and its mechanism may be related to the promotion of b FGF and EGF expression.
【作者单位】：湖南省人民医院老年医学研究所;湖南省人民医院康复医学科;
【基金】：湖南省2013年第二批省级科技计划项目(No.2013FJ3117) 2012年湖南省自然科学常德联合基金计划项目(No.12JJ9029)
【分类号】：R743.3
【正文快照】： 偏瘫是脑卒中最为严重的后遗症,主要表现为患者运动障碍[1]。对于脑卒中治疗不仅需要挽救患者生命,而且更加需要改善患者生活自理能力和生活质量[2]。强制性运动疗法(CI-MT)是主要用于脑卒中恢复期上肢功能训练康复的治疗技术,效果显著[3]。临床研究显示经过早期CIMT可能通过

【参考文献】