蛛网膜下腔内注射TSA对RTX诱导神经病理性疼痛的影响及机制

发布时间：2018-02-04 05:04

本文关键词： 神经病理性疼痛曲古菌素A 脑源性神经营养因子树酯毒素大鼠　出处：《山东医药》2017年29期 　论文类型：期刊论文

【摘要】：目的探讨蛛网膜下腔内注射古菌素A(TSA)对树酯毒素(RTX)诱导的神经病理性疼痛的影响及机制。方法雄性健康SD大鼠24只,用随机数字表法均分成Vehicle组、RTX组、RTX+DMSO组、RTX+TSA组。除Vehicle组外均进行神经病理性疼痛造模处理,并于建模前1周对RTX+DMSO组及RTX+TSA组进行蛛网膜下腔置管。Vehicle组单次腹腔注射RTX的溶媒1.5 m L;RTX组单次腹腔注射RTX 210μg/kg;RTX+DMSO组建模前60 min及建模后每天蛛网膜下腔内注射5%的DMSO 10μL,连续注射7 d;RTX+TSA组建模前60 min及建模后每天蛛网膜下腔内注射TSA 0.5μg/kg,其溶媒为5%的DMSO。建模前各组大鼠采用von Frey丝检测后肢足底基础痛阈值,建模后第1、3、5、7天测定机械痛阈值。于最后1次蛛网膜下腔内注射后2 h取材,采用qRT-PCR方法测定脊髓及脊神经背根节脑源性神经营养因子(BDNF)mRNA的表达。结果与Vehicle组相比,RTX组、RTX+DMSO组、RTX+TSA组在建模后第3、5、7天,机械痛阈值降低(P均0.05);与RTX组和RTX+DMSO组相比,RTX+TSA组第5、7天建模机械痛阈值升高(P均0.05);RTX组与RTX+DMSO组各时点相比,P均0.05。与Vehicle组相比,RTX组和RTX+DMSO组脊髓及脊神经背根节BDNF mRNA表达下调(P均0.05);与RTX组和RTX+DMSO组比较,RTX+TSA组脊髓及脊神经背根节BDNF mRNA表达上调(P均0.05);RTX组与RTX+DMSO组相比,P均0.05。结论蛛网膜下腔内注射TSA可通过上调BDNF的表达缓解RTX诱导的大鼠神经病理性疼痛。
[Abstract]:Objective to investigate the effect and mechanism of subarachnoid injection of actinomycin A (TSA) on neuropathic pain induced by dendritic toxin (RTX). Methods 24 male healthy SD rats were enrolled in this study. All patients were randomly divided into Vehicle group (Vehicle group), DMSO group (DMSO group) and RTX TSA group. All patients were treated with neuropathic pain modeling except Vehicle group. 1 week before modeling, the RTX DMSO group and the RTX TSA group were given a single intraperitoneal injection of 1.5 mL of RTX solute in the subarachnoid catheterization .Vehicle group. In RTX group, RTX 210 渭 g / kg was injected intraperitoneally. In RTX DMSO group, 5% DMSO (10 渭 L) was injected into subarachnoid space at 60 min before modeling and 7 days after modeling. TSA 0.5 渭 g / kg was injected into the subarachnoid space of RTX TSA group 60 min before modeling and 60 min after modeling. Before modeling, von Frey wire was used to detect the threshold of basal pain in hind limbs. The mechanical pain threshold was measured at 7 days. The samples were obtained 2 hours after the last subarachnoid injection. The expression of brain-derived neurotrophic factor (BDNF) in spinal cord and dorsal root ganglion of spinal cord was determined by qRT-PCR. In the RTX DMSO group, the mechanical pain threshold decreased by 0.05U on the 7th day after modeling. Compared with RTX group and RTX DMSO group, the threshold of mechanical pain in the TSA group increased by 0.05g on the 7th day. There was significant difference between RTX group and RTX DMSO group at different time points (P < 0.05), and compared with Vehicle group (P < 0.05). In RTX group and RTX DMSO group, the expression of BDNF mRNA in spinal cord and dorsal root ganglion was down-regulated (P 0.05). Compared with RTX group and RTX DMSO group, the expression of BDNF mRNA in spinal cord and dorsal root ganglion of spinal nerve was up-regulated in TSA group (P < 0.05). Conclusion Intraarachnoid injection of TSA can alleviate the neuropathic pain induced by RTX in rats by up-regulating the expression of BDNF.
【作者单位】：苏州大学附属第一医院;
【基金】：国家自然科学基金资助项目(81471136) 江苏省青年医学人才基金资助项目(QNRC2016740)
【分类号】：R741
【正文快照】： 带状疱疹后遗神经痛(PHN)继发于水痘带状疱疹病毒感染,是最常见和最严重的并发症,严重影响患者的生活质量,且其发病率近年随人口老龄化增加而升高[1]。脑源性神经营养因子(BDNF)作为疼痛研究的靶点越来越得到关注。BDNF与表观遗传学的关系近年也得到验证。研究表明,曲古菌素A(

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