比较额颞叶及小脑皮层电惊厥阈值与神经损害的实验研究

发布时间：2018-02-06 02:22

本文关键词： 癫痫皮层惊厥阈值神经元损害电点燃模型大鼠　出处：《山西医科大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的:通过建立大鼠的皮层惊厥阈值模型测定并比较额叶、颞叶及小脑皮层的惊厥阈值;比较额颞叶及小脑皮层惊厥阈值稳定后的海马CA3区神经元变化。方法:选择健康雌性SD大鼠,体重180-220g之间,分别在额叶皮层、颞叶皮层、小脑皮层安放电极造模,作为刺激组,对照组仅安放电极不予刺激,使用直流电每日2次刺激大鼠额叶、颞叶、小脑皮层,当刺激组惊厥阈值稳定后,处死各组大鼠,用Nissl染色法观察海马神经元损害情况。结果:1、实验开始时,大鼠三个刺激组皮层的惊厥阈值均很高,额叶组阈值波动在1100μA上下,颞叶组阈值波动在1200μA上下,小脑组阈值波动在2100μA上下,随着刺激时间的延长,各组大鼠的皮层阈值不断降低,并最终稳定在一个基线水平,但其基线各不相同,额叶组阈值波动于520μA左右,颞叶组稳定在440μA左右,小脑组稳定在1300μA左右,通过单因素方差分析,比较各组稳定后的惊厥阈值,额颞叶组的P0.05,小脑与额颞叶组的P0.05;2、随着刺激时间的延长,刺激组大鼠脑海马CA3区神经元呈现不同程度的缺失,且排列紊乱,对照组高倍镜下观察神经元数目约(36.33±6.13)个,额叶组、颞叶组、小脑组神经元数目分别为:(22.79±1.01)个、(17.78±1.40)个、(26.00±1.70)个,与对照组比较,额叶、颞叶、小脑组神经元个数减少,三组间比较具有统计学差异(P0.05);3、电刺激小脑皮层时大鼠出现的症状与额、颞叶皮层的表现不尽相同,额颞叶组均表现出典型的痫样抽搐,如点头、动须、四肢及全身强直-阵挛行为,而小脑组表现为全身的强直痉挛以及奔跑、跳跃等。结论:1、颞叶与额叶皮层对电刺激比小脑皮层更敏感,更容易形成癫痫病灶,而颞叶与额叶之间对刺激的易感性大致相同;2、海马神经元损害严重程度:颞叶额叶小脑。
[Abstract]:Objective: to determine and compare the threshold of convulsion in frontal lobe temporal lobe and cerebellar cortex by establishing a rat model of cortical convulsion threshold. To compare the changes of hippocampal CA3 neurons in frontal temporal lobe and cerebellar cortex after the convulsion threshold was stabilized. Methods: healthy female SD rats weighing 180-220 g were selected respectively in frontal cortex and temporal cortex. The cerebellar cortex was set up as a model, and the control group was treated only with electrodes without stimulation. The frontal lobe, temporal lobe and cerebellar cortex were stimulated by DC twice a day, when the threshold of convulsion was stable in the stimulation group. The damage of hippocampal neurons was observed by Nissl staining. Results: at the beginning of the experiment, the cortical convulsion thresholds of the three stimulation groups were all very high. The threshold of frontal lobe group fluctuated around 1100 渭 A, temporal lobe group about 1200 渭 A, cerebellar group about 2100 渭 A, with the extension of stimulation time. The cortical threshold of each group decreased continuously, and finally stabilized at a baseline level, but the baseline was different. The threshold of frontal lobe group fluctuated around 520 渭 A, and that of temporal lobe group was about 440 渭 A. The cerebellar group was stable at about 1300 渭 A. the threshold of convulsion was compared between the frontotemporal lobe group and the cerebellar and frontotemporal lobe group by univariate ANOVA. 2, with the prolongation of the stimulation time, the neurons in the CA3 area of the brain of the rats in the stimulation group showed different degrees of deletion and disordered arrangement. The number of neurons was about 36.33 卤6.13 in the control group, 22.79 卤1.01 in the frontal lobe group, temporal lobe group and cerebellar group, respectively. Compared with the control group, the number of neurons in the frontal lobe, temporal lobe and cerebellum decreased. There was statistical difference among the three groups (P 0.05). 3. The symptoms of cerebellar cortex were different from those of frontotemporal cortex. In frontotemporal lobe group, typical epileptiform convulsions were observed, such as nodding, motor whiskers, limbs and whole body tonic-clonic behavior. The cerebellar group showed systemic ankylosis spasm, running and jumping. Conclusion 1, temporal lobe and frontal cortex are more sensitive to electrical stimulation than cerebellar cortex, and epileptic foci are more easily formed. The susceptibility to stimulation was similar between temporal lobe and frontal lobe. 2, the severity of hippocampal neuron damage: temporal frontal lobe cerebellum.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R742.1

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