冬凌草甲素诱导胶质瘤SHG44细胞凋亡及其分子机制的研究

发布时间：2018-02-10 14:21

本文关键词： 冬凌草甲素胶质瘤 SHG细胞细胞凋亡　出处：《中国医科大学学报》2017年07期 　论文类型：期刊论文

【摘要】：目的研究冬凌草甲素诱导胶质瘤SHG44细胞凋亡及其分子机制。方法采用CCK-8比色法绘制生长曲线,冬凌草甲素分别以0、1.25、2.5、5、10、20、40μmol/L观察对胶质瘤SHG44细胞生长活性的影响;Hoehst33258和TUNEL染色法观察细胞形态的变化,流式细胞仪检测细胞凋亡情况;通过Western blotting分析凋亡相关蛋白Caspase-3、cleaved Caspase-3、Bax和Bcl-2在胶质瘤SHG44细胞中蛋白的表达情况。结果冬凌草甲素作用胶质瘤SHG44细胞24 h和48 h后,细胞的增殖受到明显抑制,且24 h和48 h细胞半抑制率(IC50)的浓度分别是7.865和4.74μmol/L;Hoechst33258和TUNEL染色结果发现形态发生明显改变,出现了典型的细胞凋亡变化;Western blotting检测结果显示冬凌草甲素诱导后,下调凋亡抑制蛋白Bcl-2的表达,并激活了凋亡蛋白Caspase-3和Bax的表达。结论冬凌草甲素对胶质瘤SHG44细胞具有抑制增殖及诱导凋亡的作用,同时调控凋亡相关蛋白Caspase-3、Bcl-2和Bax的表达。
[Abstract]:Objective to study the apoptosis of glioma SHG44 cells induced by oridonin and its molecular mechanism. Methods CCK-8 colorimetric method was used to draw the growth curve. The effect of oridonin on the growth activity of glioma SHG44 cells was observed by 0 ~ (1.25) ~ 2.5A ~ (2. 5) ~ (10) ~ (20) ~ (20) 渭 mol/L. The morphologic changes of glioma SHG44 cells were observed by Hoehst33258 and TUNEL staining, and the apoptosis of glioma cells was detected by flow cytometry (FCM). The expression of apoptosis-related protein Caspase-3 cleaved caspase-3mBax and Bcl-2 in glioma SHG44 cells was analyzed by Western blotting. Results the proliferation of glioma SHG44 cells was significantly inhibited by oridonin for 24 h and 48 h. At 24 h and 48 h, the concentration of IC50 was 7.865,4.74 渭 mol / L Hoechst33258 and TUNEL, respectively. The morphological changes were observed. The typical changes of apoptosis were observed. Western blotting analysis showed that oridonin was induced by oridonin. Conclusion oridonin can inhibit the proliferation and induce apoptosis of glioma SHG44 cells, and regulate the expression of apoptosis related proteins Caspase-3, Bcl-2 and Bax.
【作者单位】：西安市第三医院神经外科;西安市中心医院神经外科;咸阳市中心医院神经外科;西安交通大学第一附属医院神经外科;
【基金】：国家自然科学基金(31301216)
【分类号】：R739.41

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