紫杉醇对实验性自身免疫性脑脊髓炎大鼠CD28、CTLA-4表达及BAFF的影响

发布时间：2018-02-12 08:20

本文关键词： 紫杉醇实验性自身免疫性脑脊髓炎 CD CTLA- B细胞刺激因子　出处：《中国比较医学杂志》2017年05期 　论文类型：期刊论文

【摘要】：目的探讨紫杉醇对实验性自身免疫性脑脊髓炎(experimental autoimmunencephalomyelitis,EAE)大鼠脑组织中CD28、CTLA-4表达及脾组织上清液中B细胞刺激因子(BAFF)含量及其意义。方法按随机数字法将50只Wistar大鼠分为5组:紫杉醇(PTX)小、中、大3个剂量组(PTX剂量分别为1 mg/kg,2 mg/kg,4 mg/kg)、正常对照组,EAE对照组,每组10只。用豚鼠脊髓制成GPSCH并与CFA等体积混合制成免疫抗原,注入大鼠双足侧足垫(2 m L/kg)进行EAE造模,自造模后,PTX各剂量组腹腔注射紫杉醇,连续10 d。正常对照组及EAE对照组均给予腹腔注射0.9%NaCl 2 m L。采用脑组织学评分评估实验大鼠脑组织炎症浸润情况,取大鼠脑组织采用流式细胞仪测定脑组织CD28、CTLA-4含量,取脾组织并制成上清液,采用ELISA测定脾组织中BAFF含量。结果 PTX各剂量组脑组织评分均低于EAE对照组,各组间差异有显著性(P0.01);PTX各剂量组大鼠脑组织中CD28表达均低于EAE对照组,各组间差异有显著性(P0.01);CTLA-4表达均高于EAE对照组,各组间差异有显著性(P0.01);脾组织上清液中BAFF含量均低于EAE对照组,各组间差异有显著性(P0.01)。结论 PTX可降低EAE大鼠神经功能障碍评分,其机制可能涉及调节大鼠脑组织中CD28、CTLA-4的表达及脾组织上清液中BAFF含量,从而发挥对EAE的防治作用。
[Abstract]:Objective to investigate the effect of paclitaxel on the expression of CD28CTLA-4 in the brain of experimental autoencephalomyelitis (EAE) rats and the content of B cell stimulating factor (BAFF) in the supernatant of splenic tissue. Methods 50 Wistar rats were randomly divided into two groups. Group 5: paclitaxel PTX was small, In the middle and high dose groups, the dose of PTX was 1 mg / kg ~ 2 mg / kg ~ (2) mg / kg ~ (-1) 路kg ~ (-1) respectively, while that of normal control group was 10 rats in each group. GPSCH was made from guinea pig spinal cord and mixed with CFA in the same volume to make immune antigen, and then injected into bilateral foot pad of rats (2 mL / kg) to make EAE model. Paclitaxel was injected intraperitoneally for 10 days in each dose of PTX group. The normal control group and EAE control group were given intraperitoneal injection of 0.9 NaCl 2 mL. The inflammatory infiltration of brain tissue was evaluated by brain histological score. The content of CD28 CTLA-4 in brain tissue was measured by flow cytometry, and the supernatant was obtained from spleen tissue. The content of BAFF in splenic tissue was determined by ELISA. Results the scores of brain tissue in each PTX group were lower than those in EAE control group. The expression of CD28 in brain tissue of rats in each dose group was lower than that in EAE control group, and the expression of CTLA-4 in each group was higher than that in EAE control group. The content of BAFF in the supernatant of splenic tissue was lower than that in the control group of EAE, and the difference was significant among the groups. Conclusion PTX can reduce the score of neurological dysfunction in EAE rats. The mechanism may involve regulating the expression of CD28 CTLA-4 in rat brain and the content of BAFF in the supernatant of splenic tissue, so as to play a role in the prevention and treatment of EAE.
【作者单位】：西南医科大学附属医院神经内科;
【基金】：泸州市科技创新苗子项目(2016-R-70 20/24) 西南医科大学附属医院院级课题
【分类号】：R744.51

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