外周血白细胞端粒长度与大动脉粥样硬化性脑梗死及颈动脉粥样硬化斑块稳定性的相关研究

发布时间：2018-02-24 12:49

本文关键词： 大动脉粥样硬化性脑梗死颈动脉斑块稳定性端粒外周血白细胞端粒长度　出处：《安徽医科大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的:探讨外周血白细胞端粒长度与大动脉粥样硬化性脑梗死是否具有相关性,以及更进一步探讨其是否与动脉粥样硬化斑块稳定性相关。方法:依据我国高山教授等提出的脑梗死病因分型(Chinese ischemic stroke subclassification,CISS),选取2015年12月至2016年9月省立医院神经内科病区收住的起病72小时内前循环大动脉粥样硬化型急性脑梗死患者70例作为病例组,并同期从安徽省立医院体检中心选取性别年龄与之相匹配的健康体检者68例作为对照组。详细记录入组对象的一般资料及血液学资料。所有入组对象均于入组次晨空腹抽取静脉血2ml,通过实时荧光定量PCR法测定病例组及对照组的外周血白细胞端粒长度。所有患者均使用二维彩色多普勒超声检测仪对颈动脉粥样硬化斑块的类型进行详尽的检查和分析,依据超声结果将70例前循环大动脉粥样硬化型急性脑梗死患者分为不稳定颈动脉粥样硬化斑块组(40例)和稳定颈动脉粥样硬化斑块组(30例)。比较病例组及对照组间外周血白细胞端粒长度是否有差异同时更进一步比较稳定斑块组及不稳定斑块组是否存在差异。结果:1.病例组与对照组的外周血白细胞端粒长度统计学上存在明显差异,病例组外周血长度明显短于对照组。2.不稳定斑块组与稳定斑块组外周血白细胞端粒长度存在统计学差异,不稳定斑块组的外周血白细胞端粒长度短于稳定斑块组。结论:1.外周血白细胞端粒端粒长度与大动脉粥样硬化性脑梗死存在相关性,且外周血白细胞端粒长度可能成为预测急性动脉粥样硬化性脑梗死的生物指标。2.进一步发现外周血白细胞端粒长度可能与斑块的稳定性存在相关性,这可能是其促进大动脉粥样硬化性脑梗死发生的机制。
[Abstract]:Objective: to investigate the relationship between telomere length and atherosclerotic cerebral infarction in peripheral blood. Methods: according to the classification of the etiology of cerebral infarction put forward by Professor Alpine et al, Chinese ischemic stroke subclassification was selected from December 2015 to September 2016 in the nerves of the provincial hospital, and whether it was related to the stability of atherosclerotic plaques or not. Methods: according to the classification of the etiology of cerebral infarction proposed by Professor Alpine et al. A total of 70 patients with acute cerebral infarction with anterior circulation arteriosclerosis within 72 hours from the onset of the disease were selected as the case group. At the same time, 68 healthy persons matched with sex and age were selected from the center of Anhui Provincial Hospital as control group. The general data and hematological data of the subjects were recorded in detail. All the subjects were included in the group. The telomere length of peripheral blood leucocytes was measured by real-time fluorescence quantitative PCR in all patients with carotid atherosclerotic plaque by two-dimensional color Doppler ultrasound. Type for detailed examination and analysis, According to the results of ultrasound, 70 patients with acute cerebral infarction were divided into unstable carotid atherosclerotic plaque group (40 cases) and stable carotid atherosclerotic plaque group (30 cases). There were significant differences in telomere length between peripheral blood leukocytes and between stable plaque group and unstable plaque group. Results there were significant differences in telomere length between case group and control group. The length of peripheral blood in the case group was significantly shorter than that in the control group. 2. There was significant difference in telomere length between the unstable plaque group and the stable plaque group. The telomere length of peripheral blood leukocytes in unstable plaque group was shorter than that in stable plaque group. Conclusion 1. There is a correlation between telomere length and atherosclerotic cerebral infarction. The telomere length of peripheral blood leukocytes may be a biological index to predict acute atherosclerotic cerebral infarction. Further, it was found that telomere length in peripheral blood leukocytes may be related to plaque stability. This may be the mechanism of promoting atherosclerotic cerebral infarction.
【学位授予单位】：安徽医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R743.3

【相似文献】