低频电刺激脚桥核对大鼠苍白球内侧部神经元自发放电的影响及其机制

发布时间：2018-02-27 15:48

本文关键词： 帕金森病电刺激脑桥核苍白球微电泳谷氨酸 γ氨基丁酸　出处：《辽宁医学院》2014年硕士论文　论文类型：学位论文

【摘要】：目的通过观察低频电刺激帕金森病（Parkinsons’s disease，PD）模型大鼠脚桥核（pedunculopontine nucleus，PPN）及微电泳神经递质对苍白球内侧部（GPi）神经元放电的影响，探讨低频电刺激PPN治疗PD的作用机制。为临床采用电刺激治疗帕金森病提供实验依据。方法将30只SD大鼠随机分为对照组（10只）和PD模型组（20只）。大鼠脑右侧炓质致密部注入6-羟基多巴胺建立PD模型；自行拉制7管玻璃微电极，采用细胞外单位记录方法观察记录低频电刺激PPN、微电泳谷氨酸（Glu）及其受体阻断剂MK-801、γ-氨基丁酸（GABA）及其受体阻断剂荷包牡丹碱（BIC）、乙酰胆碱（ACh）及其受体阻断剂阿托品（ATR）对PD大鼠GPi神经元自发放电的影响。结果 PD组大鼠GPi神经元基础放电频率较对照组增高（P＜0.05）；低频电刺激PPN，对照组及PD组大鼠GPi神经元反应均以抑制为主，且平均放电频率均较刺激前明显降低（P＜0.01）；微电泳Glu、BIC和ACh对神经元有兴奋作用，，而微电泳MK-801、GABA和阿托品对神经元有抑制作用。在微电泳BIC的兴奋作用的基础上低频电刺激PPN使GPi神经元放电频率增高，而在微电泳MK-801和ATR抑制作用的基础上低频刺激PPN使GPi神经元放电频率进一步降低。结论低频电刺激PPN可抑制大鼠GPi神经元的放电活动，可能是通过调节投射到GPi神经元的Glu、GABA和Ach能神经通路实现的，而且GABA能作用更加明显。
[Abstract]:Purpose. To observe the effects of low frequency electrical stimulation of parkinsonssus disease (PDD) on the discharge of GPiG neurons in the medial globus pallidus of the rat model of Podonus pedunculata (PPN) and microelectrophoretic neurotransmitters (microelectrophoretic neurotransmitters). To explore the mechanism of low frequency electrical stimulation (PPN) in the treatment of PD and to provide experimental evidence for clinical treatment of Parkinson's disease. Method. Thirty SD rats were randomly divided into control group (n = 10) and PD model group (n = 20). The low frequency electrical stimulation (PPN, microelectrophoresis glutamate Glu), its receptor blockers MK-801, 纬 -aminobutyric acid (GABA) and its receptor blockers bicarbonate, acetylcholine (ache) and its receptor blocker atropine (ATR) were observed by extracellular unit recording method. Effect on spontaneous discharges of GPi neurons in PD rats. Results. The basal discharge frequency of GPi neurons in PD group was higher than that in control group (P < 0.05), and the response of GPi neurons to low frequency electrical stimulation was mainly inhibited in control group and PD group. The average discharge frequency was significantly lower than that before stimulation (P < 0.01), and microelectrophoretic gel electrophoresis (GluBIC) and ACh could excite neurons. On the other hand, microelectrophoretic MK-801GABA-GABA and atropine inhibited neurons. On the basis of the excitatory effect of microelectrophoretic BIC, low frequency electrical stimulation of PPN increased the firing frequency of GPi neurons. On the basis of inhibition of MK-801 and ATR by microelectrophoretic, low frequency stimulation of PPN further decreased the firing frequency of GPi neurons. Conclusion. Low frequency electrical stimulation of PPN could inhibit the discharges of GPi neurons in rats, possibly by regulating the Glu-GABA and Ach energy pathways projected to GPi neurons, and the effect of GABA was more obvious.
【学位授予单位】：辽宁医学院
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R742.5

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