超声微泡对碱性成纤维生长因子转染大鼠损伤面神经的修复作用研究

发布时间：2018-03-02 18:33

本文选题：超声微泡　切入点：碱性成纤维生长因子　出处：《重庆医学》2017年13期 　论文类型：期刊论文

【摘要】：目的应用超声微泡介导碱性成纤维生长因子(bFGF)基因进行受损面神经(大鼠模型)修复,研究其可行性及有效性。方法将40只SD大鼠制作面神经损伤模型,并分成4组,每组10只;A组:bFGF+超声+微泡组;B组:bFGF+微泡组;C组:bFGF+超声组;D组:单纯手术组(PBS)。于bFGF基因转染后第1、10、20、28天,观察各组大鼠一般状况,检测损伤面神经传导速度、潜伏期及动作电位波幅。提取损伤处面神经组织后采用RT-PCR检测mRNA的表达,Western blot检测bFGF蛋白表达。结果转染后第20天,A组大鼠术侧有少量胡须可观察到细微摆动;转染后第28天,A组大鼠一般状况较B、C、D 3组恢复得更好。A组面神经损伤后修复的神经电生理表现优于B、C、D 3组;A组bFGF mRNA和蛋白表达量明显高于B、C、D 3组(P0.05)。结论超声微泡介导bFGF有助于面神经损伤的修复。
[Abstract]:Objective to study the feasibility and effectiveness of the repair of injured facial nerve by ultrasound microbubble mediated basic fibroblast growth factor (bFGF) gene in rats. Methods 40 SD rats were divided into 4 groups. 10 rats in each group were divided into two groups: group A (n = 10), group B (n = 10), group B (n = 10), group C (n = 10): group C (n = 10): group D: simple operation group (n = 10). After transfection of bFGF gene, the general status of each group was observed and the conduction velocity of injured facial nerve was measured. The expression of mRNA was detected by RT-PCR and the expression of bFGF protein was detected by Western blot. Results on the 20th day after transfection, a small amount of whiskers could be observed in a small amount of whiskers of rats in group A. On the 28th day after transfection, the general condition of the rats in group A was better than that in group A. The electrophysiological expression of bFGF mRNA and protein in group A was better than that in group A after facial nerve injury. Conclusion the expression of bFGF mRNA and protein in group A is significantly higher than that in group B, C, D 3. Conclusion Ultrasonic microbubbles are better than those in group A. Mediating bFGF is helpful to repair facial nerve injury.
【作者单位】：重庆医科大学附属第二医院耳鼻咽喉头颈外科;
【基金】：重庆市卫生局自然科学基金重点项目(2012-1-041) 重庆市教委科技项目(KJ1600219)
【分类号】：R745

【相似文献】