骨髓单个核细胞对脑梗死大鼠炎症反应的影响

发布时间：2018-03-06 17:50

本文选题：骨髓单个核细胞　切入点：脑梗死　出处：《中风与神经疾病杂志》2017年04期 　论文类型：期刊论文

【摘要】：目的探讨骨髓单个核细胞(BMMNCs)是否通过抑制脑梗死大鼠脑内的炎症反应来发挥神经保护作用。方法选择健康雄性SD大鼠,体质量为250~300 g,线栓法制备永久性大脑中动脉闭塞模型(p MCAO),造模成功后,随机分为4组:假手术组(Sham组)、模型组(Model组)、溶剂组(Vehicle组)、骨髓单个核细胞组(BMMNCs组)。其中BMMNCs组于造模24 h后经尾静脉注射含1×107BMMNCs的PBS细胞悬液200μl;溶剂组注射等量的PBS溶液,分别于24 h、72 h、7 d采用Zea-Longa法进行神经功能评分;干湿重法检测脑组织含水量;TTC法测定脑梗死体积;免疫组化、Western blot技术检测炎性介质环氧化酶-2(COX-2)、白介素-1β(IL-1β)的表达情况。结果 (1)各时间点模型组Zea-Longa评分、脑组织含水量和脑梗死体积均明显高于假手术组(P0.05),BMMNCs组低于模型组,高于假手术组(P0.05),模型组与溶剂组相比无差异(P0.05);(2)各时间点模型组COX-2和IL-1β水平明显均高于假手术组(P0.05),BMMNCs组低于模型组,高于假手术组(P0.05),模型组与溶剂组相比无差异(P0.05)。结论骨髓单个核细胞移植可通过抑制梗死后脑内免疫炎症反应,显著改善脑梗死大鼠的神经功能,这一治疗作用可能与下调COX-2、IL-1β的表达有关。
[Abstract]:Objective to investigate whether bone marrow mononuclear cells (BMMNCs) play a neuroprotective role by inhibiting inflammatory response in the brain of rats with cerebral infarction. Methods healthy male SD rats were selected. The permanent middle cerebral artery occlusion (MCAO) model was established by the method of thread embolization. The rats were randomly divided into four groups: sham group, model group, vehicle group, bone marrow mononuclear cell group and bone marrow mononuclear cell group. The BMMNCs group was injected with PBS cell suspension containing 1 脳 107 BMMNCs through tail vein for 24 hours, and the solvent group was injected with the same amount of PBS solution. The neurological function was evaluated by Zea-Longa method at 24 hours and 72 hours after 7 days, and the volume of cerebral infarction was measured by dry and wet weight method. Immunohistochemistry Western blot technique was used to detect the expression of inflammatory mediator cyclooxygenase-2 (COX-2) and interleukin-1 尾 (IL-1 尾). Results: the Zea-Longa score, brain water content and cerebral infarction volume in model group were significantly higher than those in sham operation group (P 0.05) and the level of BMMNCs in model group was significantly higher than that in model group. The levels of COX-2 and IL-1 尾 in the model group were significantly higher than those in the sham operation group and the model group were significantly higher than those in the model group. There was no difference between the model group and the solvent group. Conclusion Bone marrow mononuclear cell transplantation can significantly improve the neural function of rats with cerebral infarction by inhibiting the immune inflammation after infarction. This therapeutic effect may be related to the down-regulation of the expression of COX-2 and IL-1 尾.
【作者单位】：郑州大学第五附属医院神经内科;
【基金】：国家自然科学基金面上项目(81271284)
【分类号】：R743.33

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