缺血性卒中患者血清Klotho蛋白表达水平对NO的调控作用与Klotho基因多态性研究

发布时间：2018-03-09 13:45

本文选题：缺血性卒中　切入点：Klotho　出处：《吉林大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的:寻找新的缺血性卒中的危险因素,明确缺血性卒中患者klotho蛋白对NO水平的影响。方法:收集2016年12月-2017年3月于吉林大学第二医院就诊的人群,分为两组,缺血性卒中组158例,对照组104例。采集研究对象的临床资料,采用等位基因特异性引物PCR检测Klotho基因多态性位点的基因型,采用ELISA法测定血清Klotho蛋白和NO的浓度。结果:1.缺血性卒中组在年龄、性别、冠心病史、甘油三酯、总胆固醇、低密度脂蛋白、F352V和C370S方面与对照组相比无显著差异(p0.05);在高血压病病史、糖尿病病史、吸烟史、饮酒史、空腹血糖、同型半胱氨酸方面高于对照组(p0.05);在HDL、klotho蛋白、NO水平方面低于对照组(p0.05);缺血性卒中组G-395A、C1818T的基因型分布与对照组相比更加趋向于杂合型和突变型(p0.05)。2.发生缺血性卒中的风险,高血压病患者较非高血压病患者增加1.8倍,吸烟者较非吸烟者增加2.4倍,饮酒者较非饮酒者增加2.6倍,同型半胱氨酸每升高1个单位风险升高3.5%,NO浓度每升高1个单位风险降低0.7%。3.Klotho蛋白与NO呈正相关,相关系数r=0.3294(p0.0001)。回归方程为Y^(NO)=78.3089+0.068*X(Klotho),决定系数R2=0.0427。结论:1.Klotho基因G-395A、C1818T等位基因多态性与缺血性卒中可能具有相关性。2.Klotho基因F352V、C370S等位基因多态性与缺血性卒中不具有相关性。3.血清NO是缺血性卒中的保护因素。4.缺血性卒中患者klotho蛋白对NO水平具有正向调控作用,klotho蛋白和NO浓度的降低可能与缺血性卒中的发生发展有关。5.高血压病、糖尿病、烟酒史、高同型半胱氨酸是缺血性卒中的危险因素。
[Abstract]:Objective: to find out the new risk factors of ischemic stroke and to determine the effect of klotho protein on no level in ischemic stroke patients. Methods: from December 2016 to March 2017, the patients in the second Hospital of Jilin University were divided into two groups. 158 cases of ischemic stroke group and 104 cases of control group. The clinical data were collected and allele-specific primer PCR was used to detect the genotype of Klotho gene polymorphism locus. The concentration of serum Klotho protein and no were determined by ELISA method. Results: 1. Age, sex, history of coronary heart disease, triglyceride, total cholesterol in ischemic stroke group, There was no significant difference between low density lipoprotein F352V and C370S compared with the control group (p 0.05), and the history of hypertension, diabetes, smoking, drinking, fasting blood glucose, The homocysteine level was higher than that in the control group (p 0.05); the level of no in the HDL klotho protein was lower than that in the control group (P 0.05); the genotype distribution of G-395An C1818T in the ischemic stroke group was more likely to be heterozygous and mutant than that in the control group, and the risk of ischemic stroke. The number of patients with hypertension increased 1.8 times, the number of smokers increased 2.4 times than that of non-smokers, and the number of drinkers increased 2.6 times than that of non-drinkers. There was a positive correlation between Klotho protein and no in every increase of homocysteine by 3. 5%, 0. 7%, 0. 7%, 3. 3. Klotho protein. The correlation coefficient is 0.3294p0.00010.The regression equation is 78.3089 0.068XKlothotrochus, R20.0427.Conclusion the G-395Agna C1818T allele polymorphism of Klotho gene may be associated with ischemic stroke. 2. The polymorphism of Klotho gene F352VC370S allele is not correlated with ischemic stroke. Serum no is not correlated with the polymorphism of C370S allele of Klotho gene F352VC370S in ischemic stroke. The relationship between the polymorphism of G-395An C1818T allele of Klotho gene and ischemic stroke is not significant. Klotho protein has a positive effect on the level of no in ischemic stroke patients. The decrease of klotho protein and no concentration may be related to the occurrence and development of ischemic stroke .5.Hypertension. Diabetes, alcohol and tobacco history, and homocysteine are risk factors for ischemic stroke.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R743.3

【相似文献】