ADAMTS4、ADAMTS5蛋白酶在人脑胶质瘤中的表达及其临床意义

发布时间：2018-03-09 14:33

本文选题：人脑胶质瘤　切入点：ADAMTS4蛋白　出处：《桂林医学院》2017年硕士论文　论文类型：学位论文

【摘要】：目的:研究人脑胶质瘤中含血小板结合蛋白基序的解聚素金属4(A Disintegrin and Metalloprotease with Thrombospondin motifs,ADAMTS)蛋白酶与ADAMTS5蛋白酶的表达及其与胶质瘤恶性程度的相互关系及临床意义。方法:采用免疫组织化学方法检测ADAMTS4蛋白及ADAMTS5蛋白在44例不同级别脑胶质瘤、11例瘤周脑组织中的表达,计算阳性细胞百分率,并通过Image Pro Plusversion 6.0(IPP 6.0)图像分析软件测量计算其平均光密度(mean optical density,MOD)值。采用SPSS 18.0统计软件进行统计分析,对ADAMTS4及ADAMTS5蛋白在瘤周脑组织和各级别胶质瘤中的阳性表达率用χ2检验;ADAMTS4和ADAMTS5蛋白表达相关性分析采用χ2检验及关联性分析;MOD值采用独立样本t检验,均以P0.05为差异有统计学意义。结果:ADAMTS4和ADAMTS5蛋白酶在瘤周脑组织中不表达或极低表达,而在胶质瘤组织中高表达,主要表达定位于细胞外基质及细胞质中。进一步研究显示,其蛋白表达量随着肿瘤级别的升高而升高。在瘤周脑组织组、低级别胶质瘤组、高级别胶质瘤组中ADAMTS4阳性表达率分别为18.18%(2/11)、61.90%(13/21)及91.30%(21/23),其MOD值在低级别胶质瘤组和高级别胶质瘤组为0.1784±0.02153、0.2723±0.02725,差异有统计学意义(P0.05);而ADAMTS5阳性表达率分别为27.27%(3/8)、71.43%(15/21)及95.65%(22/23),其MOD值在低级别胶质瘤组和高级别胶质瘤组为0.1920±0.02654、0.2852±0.03098,差异也有统计学意义(P0.05)。ADAMTS4及ADAMTS5蛋白的共表达率为70.45%(31/44),并呈正相关(r=0.349,P0.05)。结论:(1)ADAMTS4及ADAMTS5蛋白的表达可作为判断人脑胶质瘤生物学行为的重要指标,其与胶质瘤的发展、侵袭密切相关。(2)ADAMTS4及ADAMTS5蛋白的表达量随着患者脑胶质瘤的恶性程度升高而升高,对预测胶质瘤恶性程度有一定的指导意义。(3)ADAMTS4及ADAMTS5蛋白关系密切,两者的过表达可能共同促进了胶质瘤的侵袭及迁移。
[Abstract]:Objective: to study the expression of depolymerin 4A Disintegrin and Metalloprotease with Thrombospondin motifs Thrombospondin adamtas protease and ADAMTS5 protease in human glioma and their relationship with malignant degree of glioma and its clinical significance. Immunohistochemical method was used to detect the expression of ADAMTS4 protein and ADAMTS5 protein in the surrounding brain tissues of 44 patients with different grade gliomas. The percentage of positive cells was calculated, and the mean optical density was calculated by Image Pro Plusversion 6.0) image analysis software. SPSS 18.0 statistical software was used for statistical analysis. The positive expression rate of ADAMTS4 and ADAMTS5 protein in peripheral brain tissues and gliomas was analyzed by 蠂 2 test. The correlation between ADAMTS4 and ADAMTS5 protein expression was analyzed by 蠂 2 test and correlation analysis. Results there was no or very low expression of ADAMTS5 protease in the surrounding brain tissue, but high expression in glioma tissue, mainly located in extracellular matrix and cytoplasm. The protein expression increased with the increase of tumor grade. The positive expression rate of ADAMTS4 in the high grade glioma group was 18.18 / 111.90 (13 / 21) and 91.30% 21 / 23 respectively. The MOD value in the low grade glioma group and the high grade glioma group was 0.1784 卤0.02153 卤0.2723 卤0.02725, the difference was statistically significant (P 0.05), while the positive expression rate of ADAMTS5 was 27.2727 / 32 / 38 / 71.43 / 15 / 21 and 95.6565 / 22 / 21, respectively. In glioma group and high grade glioma group, 0.1920 卤0.02654 + 0.2852 卤0.03098, the difference was also statistically significant. The coexpression rate of P0.05, ADAMTS4 and ADAMTS5 protein was 70.455.The expression of ADAMTS4 and ADAMTS5 protein could be used as an important index to judge the biological behavior of human glioma. The expression of ADAMTS4 and ADAMTS5 protein is closely related to the development and invasion of glioma, and the expression of ADAMTS4 and ADAMTS5 protein increases with the malignant degree of glioma. It has a certain guiding significance in predicting the malignant degree of glioma. The expression of ADAMTS4 and ADAMTS5 protein is closely related to the prognosis of glioma malignancy. The overexpression of both may promote the invasion and migration of gliomas.
【学位授予单位】：桂林医学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R739.41

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