维格列汀通过上调胰高血糖素样肽1发挥抗癫痫作用

发布时间：2018-03-10 04:04

本文选题：维格列汀　切入点：颞叶癫痫　出处：《南方医科大学学报》2017年01期 　论文类型：期刊论文

【摘要】：目的探讨维格列汀对戊四氮(PTZ)诱导的癫痫模型中胰高血糖素样肽1(GLP-1)及胰高血糖素样肽1受体(GLP-1R)的影响而发挥抗癫痫作用。方法脑外伤患者切除的皮质为对照组(control;n=14),颞叶癫痫(TLE)患者行癫痫灶切除的皮质为颞叶癫痫组(n=23)。90只SD雄性大鼠随机分为对照组(A组,control)、PTZ单纯致痫组(B组,PTZ-induced epilepsy)、生理盐水2 m L/kg干预组(C组,PTZ+2 m L/kg saline)、维格列汀2.5 mg/kg干预组(D组,PTZ+2.5 mg/kg vildagliptin)、维格列汀5 mg/kg干预组(E组,PTZ+5 mg/kg vildagliptin)、维格列汀10 mg/kg干预组(F组,PTZ+10 mg/kg vildagliptin)。连续腹腔注射PTZ(35 mg/kg)3周建立慢性癫痫模型,观察大鼠行为学。运用Western blotting、免疫组织化学染色检测各组GLP-1R的表达变化,运用酶联免疫吸附检测各组血清中GLP-1的表达变化,采用免疫荧光染色和ELISA对GLP-1R进行定位。结果免疫荧光染色结果显示,GLP-1R主要表达于人体脑组织以及大鼠脑组织的神经元;免疫组织化学染色和Western blotting结果显示GLP-1R在癫痫患者以及癫痫模型中表达降低(P0.05);在大鼠中,维格列汀可以有效的延长癫痫发作的潜伏期和降低癫痫发作次数;维格列汀干预后,GLP-1R的表达升高(P0.05)。ELISA结果显示GLP-1与GLP-1R的表达变化一致(P0.05)。结论维格列汀通过上调GLP-1和GLP-1R发挥抗癫痫作用。
[Abstract]:Objective to investigate the antiepileptic effects of viglutin on glucagon like peptide (GLP-1) and glucagon like peptide 1 receptor (GLP-1R) in pentylenetetrazol (PTZ) induced epilepsy model. 90 Sprague-Dawley male Sprague-Dawley rats were randomly divided into two groups: control group (control group), control group (control group), PTZ-induced epilepsyn group (PTZ-induced epilepsyn), saline 2 mL / kg intervention group, group C (PTZ 2 mL / kg salineanum), viglitine group (2.5 mg / kg). Mg/kg intervention group (group D) was treated with PTZ2.5 mg/kg Vildagliptinin, group E with viglitine 5 mg/kg, group E with PTZ5 mg/kg Vildagliptinine, group F with Veglertine 10 mg/kg, group F with PTZ10 mg/kg Vildagliptin.Conditionally, PTZ(35 mg/kg)3 Zhou Jianli chronic epilepsy model was injected intraperitoneally. Western blotting, immunohistochemical staining and enzyme linked immunosorbent assay (Elisa) were used to detect the expression of GLP-1R, and the expression of GLP-1 in serum of each group was detected by enzyme-linked immunosorbent assay (Elisa). Results Immunofluorescence staining showed that GLP-1R was mainly expressed in the neurons of human brain and rat brain. The results of immunohistochemical staining and Western blotting showed that the expression of GLP-1R in epileptic patients and epileptic models decreased P0.05.Weglertine could effectively prolong the latent period and reduce the number of epileptic seizures in rats. The results of Elisa showed that the expression of GLP-1 and GLP-1R were the same as that of P0.050.Conclusion Viaglline may play an antiepileptic effect by upregulating GLP-1 and GLP-1R.
【作者单位】：重庆医科大学附属第一医院神经外科;
【基金】：重庆市卫生局重点资助项目(2013-1-005)
【分类号】：R742.1

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