失肌皮神经与屈肘重建后供受体肌肌肉型AChR亚型表达变化

发布时间：2018-03-10 14:28

本文选题：肌皮神经损伤　切入点：屈肘重建　出处：《遵义医学院》2014年硕士论文　论文类型：学位论文

【摘要】：目的：探索失肌皮神经损伤和屈肘重建术后，肱二头肌（受体肌）和胸大肌斜部（供体肌）内肌肉型AChR亚型（ε-AChR和γ-AChR）的表达变化规律，为防治供受体肌的萎缩提供科学指导。方法：1.实验动物及分组：42只成年SD大鼠，体重200±50g，不拘雌雄，分A、B、C、D4组，，其中B、C、D组又分别分为1、2组；它们分别是A组（正常组），B1、B2组（肌皮神经切断2、4w组），C1、C2组（假移植术2、4w组），D1、D2组（重建屈肘功能术2、4w组），每组6只。2.大体解剖。3.建立失肌皮神经损伤后胸大肌斜部移位重建屈肘功能动物模型。4.肌形态学观察和称量肌湿重。5. RT-PCR检测ε-AChR和γ-AChR mRNA表达。6. Western blot检测ε-AChR和γ-AChR蛋白表达。结果：1.大体观察：胸大肌斜部移位缝合至肱二头肌腱止端时，不会伤及血管神经，能满足无张力缝合。失肌皮神经后肱二头肌进行性萎缩、色泽变淡、觅食受限；重建屈肘术后，上述变化逐渐改善，胸大肌斜部手术前后变化不大。2.肌湿重：肱二头肌A组湿重为0.38±0.01g，肌皮神经切断后，B组和C组与A组比较，进行性减轻；屈肘重建后，D组与A组比较，逐渐回升，p＜0.05。胸大肌斜部A组肌重0.37±0.01g，屈肘重建后，D1组与A组比较，下降2.70%，p＜0.05；D2组已回升，与A组比较，p＞0.05。3. mRNA表达水平：肱二头肌和胸大肌斜部A组内ε-AChR表达水平分别为0.66±0.03和0.82±0.02，γ-AChR无表达。肱二头肌内ε-AChR的表达，B、C组与A组比较，逐渐下降，γ-AChR重新表达并逐渐上调；重建屈肘术后，D组与B2组比较，ε-AChR表达明显上调，γ-AChR下调，C组与B2组比较，结果相反，p＜0.05。胸大肌斜部内ε-AChR表达，D1和D2组与A组比较，分别下降了14.63%、1.22%，D1组与A组比较，p＜0.05，但D2组与A组比较，p＞0.05；γ-AChR在D1组重新表达为0.18±0.01，D2组几乎消退，仅为0.01±0.01，D2组和D1组比较，下调明显，p＜0.05。4.蛋白表达：各组各时间点蛋白表达变化规律与mRNA表达相同，除A组外，各组各时间点的蛋白表达变化幅度均低于mRNA的表达。结论：1. SD大鼠胸大肌斜部作为供体肌，建立屈肘重建术动物模型可行，能改善肱二头肌（受体肌）萎缩；2.失肌皮神经后，肱二头肌内ε-AChR mRNA和蛋白表达逐渐下调，而γ-AChR重新表达并逐渐上调；屈肘重建术后，受体肌内这两个受体亚型的表达变化相反；3.该屈肘重建术，供体肌内γ和ε-AChR仅一过性异常表达。
[Abstract]:Objective: to investigate the expression of AChR subtypes (蔚 -AChR and 纬 -AChR) in the biceps brachii (recipient muscle) and the oblique pectoralis major (donor muscle) after denervated cutaneous nerve injury and elbow flexion reconstruction, so as to provide scientific guidance for the prevention and treatment of donor muscle atrophy. Methods: 1. The experimental animals and 42 adult SD rats were divided into two groups. The weight was 200 卤50 g. The rats were divided into two groups. They were group A (normal group), group B _ (1) B _ (B _ (2)) (group C _ (1) C _ (1) C _ (2) C _ (2) C _ (2) C _ (2) C _ (2) C _ (2) C _ (2) C _ (2) C _ (2) C _ (2) C _ (2) C _ (2) C _ (2) C _ (2) C _ (2) C _ (2) C _ (2) C _ (2) C _ (2) C _ (2) C _. Observation of muscle morphology and weighing of wet muscle weight. RT-PCR was used to detect the expression of 蔚 -AChR and 纬 -AChR mRNA. 6. Western blot was used to detect the expression of 蔚 -AChR and 纬 -AChR protein. Results 1. General observation: when the oblique part of pectoralis major muscle was sutured to the end of biceps brachii tendon, it would not injure the blood vessel and nerve, and could satisfy the tension-free suture. The biceps biceps after the loss of musculocutaneous nerve gradually atrophied, the color became light, and the foraging was limited. After elbow flexion reconstruction, the above changes were gradually improved. The changes of muscle wet weight were not significant before and after operation. The wet weight of biceps brachii muscle group A was 0.38 卤0.01g, and that of group B and C decreased progressively compared with group A after transection of musculocutaneous nerve. The muscle weight of group A was 0.37 卤0.01 g in oblique part of pectoralis major, and decreased 2.70% (P < 0.05) in group D after elbow flexion reconstruction compared with group A. Compared with group A, the expression level of 蔚 -AChR in biceps brachii muscle and oblique part of pectoralis major muscle was 0.66 卤0.03 and 0.82 卤0.02respectively, but the expression of 蔚 -AChR in biceps brachii muscle was not expressed. The expression of 蔚 -AChR in biceps brachii muscle was decreased gradually compared with group A, but the expression of 纬 -AChR was gradually up-regulated. Compared with group B2, the expression of 蔚 -AChR in group D was significantly up-regulated, and the expression of 蔚 -AChR in group D was significantly higher than that in group B2 (P < 0.05). The expression of 蔚 -AChR in the oblique part of pectoralis major was significantly higher in group D than that in group B 2, and the expression of 蔚 -AChR in group D was significantly higher than that in group A (P < 0.05). The expression of 纬 -AChR in D1 group was almost disappeared to 0.18 卤0.01D 2 group, only 0.01 卤0.01 渭 g D 2 group, but P > 0.05 in D 2 group compared with A group, and the expression of 纬 -AChR in D 1 group was less than that in A group (P < 0.05), and the expression of 纬 -AChR in D 1 group was almost disappeared, only 0.01 卤0.01 渭 g D 2 group and D 1 group, P < 0.05, P < 0.05, P < 0.05, respectively. The expression of protein was decreased significantly (P < 0.05.4). The change of protein expression at each time point in each group was the same as that in mRNA, except for group A, the change range of protein expression at each time point in each group was lower than that of mRNA expression. Conclusion 1. The oblique part of the pectoralis major muscle of SD rats is feasible to establish the animal model of elbow flexion reconstruction, which can improve the atrophy of the biceps brachii (receptor muscle). 2. The expression of 蔚 -AChR mRNA and protein in the biceps brachii is gradually down-regulated after the loss of the cutaneous nerve of the biceps brachii. After elbow flexion reconstruction, the expression of these two receptor subtypes was reversed. 3. In this elbow flexion reconstruction, the expression of 纬 and 蔚 -AChR in donor muscle was only transient abnormal.
【学位授予单位】：遵义医学院
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R746

【参考文献】