结核性脑膜炎脑脊液细胞因子筛选研究

发布时间：2018-03-12 21:44

本文选题：结核性脑膜炎　切入点：细胞因子　出处：《宁夏医科大学》2014年硕士论文　论文类型：学位论文

【摘要】：背景结核性脑膜炎是常见的中枢神经系统感染性疾病之一，，具有较高的发病率和死亡率，预后差，但临床工作中仍缺乏有效的诊断手段。脑脊液中细胞因子测定在结核性脑膜炎方面的研究相对较少，利用蛋白芯片对脑脊液中细胞因子进行系统筛选国内外无相关报道。目的筛选结核性脑膜炎脑脊液中可能具有诊断意义的细胞因子。方法利用120种细胞因子蛋白芯片检测2例结核性脑膜炎、2例病毒性脑膜炎和2例非感染性神经系统疾病患者脑脊液中细胞因子，并筛选出差异性细胞因子；通过ELISA方法对30例结核性脑膜炎、16例病毒性脑膜炎和16例非感染性神经系统疾病患者脑脊液中差异性细胞因子进行检测，以进一步验证其可能诊断价值。结果1、蛋白芯片检出83种细胞因子,选取了差异显著的6种因子：单核细胞趋化蛋白-2(MCP-2)、巨噬细胞炎症蛋白-1β（MIP-1β）、巨噬细胞炎症蛋白-1α（MIP-1α）、干扰素γ（IFN-γ）、单核细胞趋化蛋白-3（MCP-3）和白细胞介素-8（IL-8）。2、ELISA检测结果：在结核性脑膜炎和病毒性脑膜炎患者脑脊液中6种细胞因子含量显著高于非感染性神经系统疾病患者，其中MCP-2（6.5±6.3pg/ml和1.5±0.5pg/ml）、IFN-γ（253.3±182.5pg/ml和8.5±2.9pg/ml）、IL-8（37134.8±27192.7pg/ml和16805.8±20108.6pg/ml）在结核性脑膜炎患者脑脊液中较病毒性脑膜炎患者脑脊液中含量显著升高(均P0.05)，而两组中MIP-1β、MIP-1α、MCP-3差异无统计学意义。结论1.蛋白芯片具有初步筛选结核性脑膜炎脑脊液中差异性细胞因子的作用； 2.ELISA方法对进一步筛选差异性细胞因子具有一定作用； 3.脑脊液中MCP-2、IFN-γ、IL-8的含量与结核性脑膜炎的诊断密切相关。
[Abstract]:Background tuberculous meningitis is one of the common infectious diseases of the central nervous system with high morbidity and mortality and poor prognosis. However, there is still a lack of effective diagnostic methods in clinical work. There are relatively few studies on the determination of cytokines in cerebrospinal fluid in tuberculous meningitis, and there are no reports about the systematic screening of cytokines in cerebrospinal fluid by using protein chips at home and abroad. Objective to screen the cytokines in cerebrospinal fluid of tuberculous meningitis. Methods the cytokines in cerebrospinal fluid (CSF) of 2 cases of tuberculous meningitis and 2 cases of viral meningitis and 2 cases of non-infectious nervous system diseases were detected by 120 kinds of cytokine protein chips and the differential cytokines were screened out. The differential cytokines in cerebrospinal fluid (CSF) of 30 cases of tuberculous meningitis and 16 cases of viral meningitis and 16 cases of non-infectious nervous system diseases were detected by ELISA method. Results 1. 83 cytokines were detected by protein chip. Six factors with significant difference were selected: monocyte chemoattractant protein (MCP-2N), macrophage inflammatory protein 1 尾 (MIP-1 尾), macrophage inflammatory protein 1 伪 (MIP-1 伪), interferon 纬 (IFN- 纬), monocyte chemoattractant protein (MCP-3) and interleukin-8IL-8. 2 Elisa: in tuberculous brain. The levels of 6 cytokines in cerebrospinal fluid of patients with viral meningitis and viral meningitis were significantly higher than those of patients with non-infectious nervous system diseases. The levels of MCP-2(6.5 卤6.3 PG / ml and 1.5 卤0.5 PG / ml IFN- 纬 in CSF of tuberculous meningitis patients were significantly higher than those of viral meningitis patients (all P 0.05g / ml and 8.5 卤2.9 PG / ml vs 8.5 卤2.9 PG / ml and 16805.8 卤20108.6pg / ml, respectively). Conclusion 1. Protein microarray has the function of screening differential cytokines in cerebrospinal fluid of tuberculous meningitis. 2. Elisa has certain effect on further screening of different cytokines. 3. The level of MCP-2 IFN- 纬 -IL-8 in cerebrospinal fluid was closely related to the diagnosis of tuberculous meningitis.
【学位授予单位】：宁夏医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R529.3

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