“养脑方”对永久性局灶性脑缺血大鼠恢复早期神经功能的影响及其机制的研究

发布时间：2018-03-13 17:49

本文选题：pMCAO　切入点：脑缺血　出处：《中南大学》2014年硕士论文　论文类型：学位论文

【摘要】：目的：观察“养脑方”对永久性局灶性脑缺血大鼠恢复早期行为学的改变,探讨其促进神经功能恢复的作用机制；为脑梗死患者早期的临床治疗提供一种新的中药干预方法。方法：采用线栓法制作永久性大脑中动脉栓塞(pMCAO)模型。术后24小时采用Longa评分法评估大鼠肢体损伤程度。本研究将实验动物完全随机分为：假手术组、pMCAO模型组、西药组和中药组。以“养脑方”作为干预手段,术后3天至14天定时灌胃给药,1次/天。分别于脑缺血3天、7天、10天和14天再次行Longa评分及横木行走试验评分。14天末次给药后处死所有大鼠。采用HE染色观察脑组织形态结构；运用逆转录-聚合酶链反应(RT-PCR)法检测皮质缺血区低氧诱导因子-1α(HIF-1α)和血管内皮生长因子(VEGF)的转录水平；运用免疫组织化学染色法观察皮质缺血区HIF-1α、 VEGF的表达。采用完全随机设计的多样本方差分析和SNK-q检验对实验数据进行统计学比较。结果：1、与pMCAO模型组相比,“养脑方”对恢复早期脑缺血大鼠左侧姿势反射有显著的改善作用；能明显提高大鼠运动协调整合的能力(P0.01)；2、中药组和西药组皮质缺血区脑组织中HIF-1α、 VEGF的表达明显高于假手术组和模型组(P=0.000,P0.01)；与西药组相比,中药组皮质缺血区脑组织VEGF的表达高(P=0.009,P0.01)；3、皮质缺血区脑组织HIF-1α、VEGF的转录水平在中药组与西药组中无统计学差异(p0.05)。结论：“养脑方”能改善pMCAO脑缺血大鼠恢复早期的神经功能,其机制可能与HIF-1α上调VEGF的表达有关。
[Abstract]:Objective: to observe the effect of "Nourishing Nao Fang" on the early behavioral changes in rats with permanent focal cerebral ischemia, to explore the mechanism of promoting the recovery of nerve function, and to provide a new Chinese medicine intervention method for the early clinical treatment of patients with cerebral infarction. Methods: the model of permanent middle cerebral artery embolization (pMCAO) was established by thread embolization. The limb injury was evaluated by Longa score 24 hours after operation. The experimental animals were randomly divided into sham operation group and pMCAO model group. Western medicine group and traditional Chinese medicine group. "Yangnao prescription" as intervention means, From 3 to 14 days after operation, the rats were given intragastric administration once a day. The Longa score and the trabecular walking test score were again performed on the 3rd day, 7th day, 10th day, and 14th day, respectively. All the rats were killed after the last administration of the drug on the 14th day. The morphology of brain tissue was observed by HE staining. The transcription levels of hypoxia inducible factor-1 伪 (HIF-1 伪) and vascular endothelial growth factor (VEGF) were detected by reverse transcription-polymerase chain reaction (RT-PCR). The expression of HIF-1 伪 and VEGF in ischemic cortex was observed by immunohistochemical staining, and the experimental data were compared statistically by means of multisample ANOVA and SNK-q test. Results compared with pMCAO model group, "Yangnao Fang" could significantly improve the left postural reflex in early cerebral ischemia rats. The expression of HIF-1 伪 and VEGF in cerebral tissue of cortex ischemia area in Chinese medicine group and western medicine group was significantly higher than that in sham operation group and model group, compared with western medicine group, the expression of HIF-1 伪 and VEGF in cerebral tissue of cerebral ischemia area in Chinese medicine group and western medicine group was significantly higher than that in sham operation group and model group. The expression of VEGF in cerebral tissue of cerebral ischemia area in Chinese medicine group was higher than that in control group. There was no significant difference in the transcription level of HIF-1 伪 -VEGF between traditional Chinese medicine group and western medicine group. Conclusion: "Nourishing Nao Fang" can improve the early recovery of neural function in rats with pMCAO cerebral ischemia, and its mechanism may be related to the up-regulation of VEGF expression by HIF-1 伪.
【学位授予单位】：中南大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R743.3

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